FastQCFastQC Report
Fri 10 Feb 2017
ERR1632270.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632270.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1553685
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7000.025.371431
ATTAGAC701.2195476E-418.53
TTGCGCG1054.799949E-717.61904718
CTTATAC13750.016.81818237
GTATCAA10350.016.8019312
TAGACGT957.0622285E-515.5789484
TATACTG1201.9364688E-615.4166665
GTATAGA1252.9601815E-614.8000011
TACGCAT750.004105270414.828
ACGGTTT750.004105270414.833
TCTTATA22550.014.76718437
TTAGACT1304.446094E-614.2307694
TTATACT1458.9149944E-714.0344834
AATACGG800.00629993513.8750015
ATACCGT800.00629993513.8750016
ATATTAG800.00629993513.8750011
GTCGGGA1601.7843558E-713.8750012
TATGCCG1203.301829E-513.87499933
TGTATAG950.001245418213.6315795
TATTAGA1102.457232E-413.4545452