FastQCFastQC Report
Fri 10 Feb 2017
ERR1632269.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632269.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182161
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT2130.11692952937236839TruSeq Adapter, Index 6 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA856.91216E-1126.1176451
CCTGGAT502.6900246E-422.1999993
ACCGTGG450.003813644920.5555558
TCAGGGC701.2120646E-418.53
TATACAC902.1320575E-618.537
GGTGTCC500.00701273218.4999988
GTATCAA1451.5097612E-1017.8620681
TCTTATA3050.017.59016437
CTCTTAT5550.016.33333437
TTATACA1059.256997E-615.85714436
GTGTCCA855.333443E-415.2352939
TCCTGAA750.004086814.82
CATCTGG750.004086814.81
AGAAGAG800.00627182813.8755
CAAACTG850.0093650613.05882331
GAGCAGC850.0093650613.0588236
CTTATAC1602.6596372E-612.7187537
TCTCTTA7500.012.08666636
TATCAAC2205.4073098E-811.7727272
ATCAACG2257.387098E-811.5111113
Produced by FastQC (version 0.11.5)