##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632269.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	182161
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.80728586250625	34.0	31.0	34.0	31.0	34.0
2	32.96664489105791	34.0	33.0	34.0	31.0	34.0
3	32.973880248790906	34.0	33.0	34.0	31.0	34.0
4	36.41779524706166	37.0	37.0	37.0	35.0	37.0
5	36.337937319184675	37.0	37.0	37.0	35.0	37.0
6	36.36873974121794	37.0	37.0	37.0	35.0	37.0
7	36.35727735354988	37.0	37.0	37.0	35.0	37.0
8	36.347203847146204	37.0	37.0	37.0	35.0	37.0
9	38.149527066715706	39.0	39.0	39.0	37.0	39.0
10	38.09059568184189	39.0	38.0	39.0	37.0	39.0
11	38.19324114382332	39.0	39.0	39.0	37.0	39.0
12	38.15579624617783	39.0	38.0	39.0	37.0	39.0
13	38.178375173610156	39.0	39.0	39.0	37.0	39.0
14	39.59272292093258	41.0	39.0	41.0	37.0	41.0
15	39.62041271183184	41.0	39.0	41.0	37.0	41.0
16	39.51972156498921	41.0	39.0	41.0	37.0	41.0
17	39.5633093801637	41.0	39.0	41.0	37.0	41.0
18	39.57952580409638	41.0	39.0	41.0	37.0	41.0
19	39.61919400969472	41.0	39.0	41.0	37.0	41.0
20	39.59458940168312	41.0	39.0	41.0	37.0	41.0
21	39.579201914789664	41.0	39.0	41.0	37.0	41.0
22	39.560130873238506	41.0	39.0	41.0	37.0	41.0
23	39.52051756413283	41.0	39.0	41.0	37.0	41.0
24	39.47838999566318	41.0	39.0	41.0	37.0	41.0
25	39.41986484483506	41.0	39.0	41.0	37.0	41.0
26	39.33918346956813	40.0	39.0	41.0	36.0	41.0
27	39.27589330317686	40.0	39.0	41.0	36.0	41.0
28	39.22739774155829	40.0	39.0	41.0	36.0	41.0
29	39.10073506403676	40.0	39.0	41.0	36.0	41.0
30	39.06223615373214	40.0	39.0	41.0	36.0	41.0
31	39.00949709323071	40.0	39.0	41.0	35.0	41.0
32	38.94857845532249	40.0	38.0	41.0	35.0	41.0
33	38.82577500123517	40.0	38.0	41.0	35.0	41.0
34	38.8303643480218	40.0	38.0	41.0	35.0	41.0
35	38.825829897727836	40.0	38.0	41.0	35.0	41.0
36	38.801576627269284	40.0	38.0	41.0	35.0	41.0
37	38.774430311647386	40.0	38.0	41.0	35.0	41.0
38	38.723634586986236	40.0	38.0	41.0	35.0	41.0
39	38.56159660959261	40.0	38.0	41.0	35.0	41.0
40	38.57272412865542	40.0	38.0	41.0	35.0	41.0
41	38.57618809734246	40.0	38.0	41.0	35.0	41.0
42	38.468903881730995	40.0	38.0	41.0	35.0	41.0
43	37.65269184951774	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	2.0
20	8.0
21	16.0
22	23.0
23	40.0
24	85.0
25	135.0
26	189.0
27	291.0
28	402.0
29	631.0
30	922.0
31	1267.0
32	1712.0
33	2481.0
34	3953.0
35	5903.0
36	10087.0
37	18835.0
38	45773.0
39	89402.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.03116473888483	17.427989525749197	12.166160703992622	27.374685031373346
2	19.67654986522911	19.39493085786749	33.40945646982615	27.519062807077255
3	20.803574859602218	20.505486904441675	28.68286845153463	30.00806978442147
4	15.939196644726369	14.935688758845197	32.22698601786332	36.898128578565114
5	17.178759449058802	33.364441345842415	32.78967506765993	16.66712413743886
6	37.28514885183986	32.29725352847206	14.618935996179205	15.798661623508876
7	31.38267796070509	28.351293635849608	19.75395391988406	20.512074483561246
8	28.433638374844232	31.94097529108865	19.07433534071508	20.551050993352035
9	27.317043714077105	13.663188058914916	18.551720730562522	40.46804749644545
10	18.09662880638556	25.447269173972476	31.775187883246144	24.680914136395828
11	38.03283908191106	20.118466631166935	20.026240523492955	21.822453763429053
12	23.32771559225081	23.474289227661245	27.768841848694287	25.429153331393657
13	32.4882933229396	18.343114058442804	22.318169092176703	26.8504235264409
14	24.235154615971584	19.00736161966612	23.14930199109579	33.6081817732665
15	26.811995981576736	25.878755606304317	20.433572499053035	26.875675913065916
16	27.23360104522922	24.15775056131664	22.19904370309781	26.409604690356332
17	25.58176558099703	24.63205625792568	23.67960211022118	26.106576050856113
18	25.833191517393956	23.826175745631613	24.195080176327536	26.1455525606469
19	26.547943851867306	23.675759355734762	24.092423735047568	25.683873057350365
20	26.661030626753256	23.673563496028237	23.663682127348885	26.00172374986962
21	27.09141912923183	23.614824248878737	23.20090469419909	26.09285192769034
22	27.174312833153092	23.59396358166677	23.325519732544286	25.906203852635855
23	26.8718331585795	23.592865651813504	23.13118614851697	26.404115041090026
24	26.616015502769528	23.70705035655272	23.278308748854034	26.398625391823714
25	26.716476084342972	23.204198483758873	23.443547191769916	26.63577824012824
26	26.09340089261697	23.27995564363393	23.853623991963154	26.773019471785947
27	26.07144229555174	23.41170722602533	23.814098517245732	26.702751961177203
28	26.304752389369845	23.43915547235687	23.735047567810895	26.521044570462394
29	26.134573262114287	23.814098517245732	23.90193290550667	26.14939531513332
30	26.268520704212207	23.651604898963004	23.783905446281036	26.295968950543752
31	26.221858685448584	23.503933333699308	23.59725737122655	26.67695060962555
32	25.698695110369396	23.479229912000925	24.023254154292083	26.7988208233376
33	25.469227771037705	23.344733504976368	24.59088388842837	26.595154835557555
34	25.848013570412988	22.84243059710915	24.574414940629445	26.73514089184842
35	25.481853964350215	22.63217703020954	25.36602236483111	26.51994664060913
36	25.52796701818721	22.406003480437633	25.151926043445084	26.91410345793007
37	24.980648986336263	21.614945021162598	26.067599541065324	27.33680645143582
38	24.21703877339277	21.64129533764088	26.993154407364912	27.14851148160144
39	24.092423735047568	21.501858246276644	27.869851395194363	26.535866623481425
40	23.72022551479186	20.985831215243657	28.631265748431332	26.66267752153315
41	23.16357507918819	21.081351112477424	28.84755792952388	26.9075158788105
42	22.410944164777312	21.27348883679822	29.486553104122176	26.829013894302296
43	21.93334467860848	20.97430295178441	29.707785969554408	27.3845664000527
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	4.5
2	6.0
3	9.5
4	13.0
5	13.0
6	20.5
7	28.0
8	25.0
9	22.0
10	42.0
11	62.0
12	62.0
13	106.0
14	150.0
15	241.0
16	332.0
17	351.5
18	371.0
19	371.0
20	483.0
21	595.0
22	638.0
23	681.0
24	884.0
25	1087.0
26	1087.0
27	1395.0
28	1703.0
29	2103.5
30	2504.0
31	2951.5
32	3399.0
33	3399.0
34	4024.5
35	4650.0
36	5293.0
37	5936.0
38	6612.5
39	7289.0
40	7289.0
41	8159.5
42	9030.0
43	9520.0
44	10010.0
45	10800.5
46	11591.0
47	11591.0
48	12119.5
49	12648.0
50	13192.5
51	13737.0
52	13983.0
53	14229.0
54	14229.0
55	14552.5
56	14876.0
57	14562.0
58	14248.0
59	13569.5
60	12891.0
61	12891.0
62	12014.0
63	11137.0
64	9897.5
65	8658.0
66	7736.0
67	6814.0
68	6814.0
69	5808.5
70	4803.0
71	4012.0
72	3221.0
73	2700.5
74	2180.0
75	2180.0
76	1788.5
77	1397.0
78	1104.0
79	811.0
80	661.0
81	511.0
82	511.0
83	405.0
84	299.0
85	220.0
86	141.0
87	102.5
88	64.0
89	64.0
90	42.5
91	21.0
92	14.0
93	7.0
94	6.0
95	5.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	182161.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.88339536075699
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.51597297518401	75.08903752180198
2	7.46342620778319	12.521150626666252
3	1.5288564906227344	3.8473702035829884
4	0.5867792778171484	1.9688415260254128
5	0.28522489069485585	1.1962816136442644
6	0.19124868256273278	0.9625553310980176
7	0.11465246836259986	0.6732206832922653
8	0.07997633452338906	0.5366949190663683
9	0.05329535979800272	0.4023536163146691
>10	0.17267392291530353	2.0700189162725064
>50	0.004933368585019785	0.3219579564175362
>100	0.002960021151011871	0.4105170858177325
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT	213	0.11692952937236839	TruSeq Adapter, Index 6 (95% over 21bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	5.489649266308376E-4
4	0.0	0.0	0.0	0.0	5.489649266308376E-4
5	0.0	0.0	0.0	0.0	5.489649266308376E-4
6	0.0	0.0	0.0	0.0	5.489649266308376E-4
7	0.0	0.0	0.0	0.0	5.489649266308376E-4
8	0.0	0.0	0.0	0.0	5.489649266308376E-4
9	0.0	0.0	0.0	0.0	5.489649266308376E-4
10	0.0	0.0	0.0	0.0	5.489649266308376E-4
11	0.0	0.0	0.0	0.0	5.489649266308376E-4
12	0.0	0.0	0.0	0.0	5.489649266308376E-4
13	0.0	0.0	0.0	0.0	5.489649266308376E-4
14	0.0	0.0	0.0	0.0	5.489649266308376E-4
15	0.0	0.0	0.0	0.0	5.489649266308376E-4
16	0.0	0.0	0.0	0.0	5.489649266308376E-4
17	0.0	0.0	0.0	0.0	5.489649266308376E-4
18	0.0	0.0	0.0	0.0	5.489649266308376E-4
19	0.0	0.0	0.0	0.0	5.489649266308376E-4
20	0.0	0.0	0.0	0.0	5.489649266308376E-4
21	0.0	0.0	0.0	0.0	5.489649266308376E-4
22	0.0	0.0	0.0	0.0	5.489649266308376E-4
23	0.0	0.0	0.0	0.0010979298532616752	5.489649266308376E-4
24	0.0	0.0	0.0	0.0016468947798925126	5.489649266308376E-4
25	0.0	0.0	0.0	0.0032937895597850253	5.489649266308376E-4
26	0.0	0.0	0.0	0.008234473899462563	5.489649266308376E-4
27	0.0	0.0	0.0	0.025252386625018528	5.489649266308376E-4
28	0.0	0.0	0.0	0.11912538907889175	5.489649266308376E-4
29	0.0	0.0	0.0	0.23440802367136765	5.489649266308376E-4
30	0.0	0.0	0.0	0.3859223434214788	5.489649266308376E-4
31	0.0	0.0	0.0	0.8964597251881578	5.489649266308376E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	85	6.91216E-11	26.117645	1
CCTGGAT	50	2.6900246E-4	22.199999	3
ACCGTGG	45	0.0038136449	20.555555	8
TCAGGGC	70	1.2120646E-4	18.5	3
TATACAC	90	2.1320575E-6	18.5	37
GGTGTCC	50	0.007012732	18.499998	8
GTATCAA	145	1.5097612E-10	17.862068	1
TCTTATA	305	0.0	17.590164	37
CTCTTAT	555	0.0	16.333334	37
TTATACA	105	9.256997E-6	15.857144	36
GTGTCCA	85	5.333443E-4	15.235293	9
TCCTGAA	75	0.0040868	14.8	2
CATCTGG	75	0.0040868	14.8	1
AGAAGAG	80	0.006271828	13.875	5
CAAACTG	85	0.00936506	13.058823	31
GAGCAGC	85	0.00936506	13.058823	6
CTTATAC	160	2.6596372E-6	12.71875	37
TCTCTTA	750	0.0	12.086666	36
TATCAAC	220	5.4073098E-8	11.772727	2
ATCAACG	225	7.387098E-8	11.511111	3
>>END_MODULE