FastQCFastQC Report
Fri 10 Feb 2017
ERR1632268.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632268.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727691
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20070.11616660618131366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11600.022.1681021
GTCTAGA3250.019.9230771
TCTAGAC3200.019.0781253
ATTCCGC1306.9667294E-1018.528
GTTCTAG3400.018.4999981
TCTATAC1750.017.971433
TAACGCC2000.017.5754
CTAGAAC3700.017.53
TAGAACT3850.017.29874
CTAGACA3500.016.9142864
TCGTTTA1901.8189894E-1216.55263130
CGACGGT1606.2937033E-1016.18757
ATACCGT1151.2430028E-616.0869566
TACCGTC1151.2430028E-616.0869567
TAATACT1504.6748028E-916.0333334
GACGTAT2450.015.85714226
ACTGATC3400.015.7794118
GTAATAC1302.5909685E-715.6538463
ACGTATG2500.015.5427
ATAACGC2500.015.543
Produced by FastQC (version 0.11.5)