##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632268.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1727691
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.67073568132265	34.0	31.0	34.0	31.0	34.0
2	32.826653608776105	34.0	31.0	34.0	31.0	34.0
3	32.833598137629934	34.0	31.0	34.0	31.0	34.0
4	36.32250848097258	37.0	37.0	37.0	35.0	37.0
5	36.220354218433734	37.0	37.0	37.0	35.0	37.0
6	36.25025597748672	37.0	37.0	37.0	35.0	37.0
7	36.24445401405691	37.0	37.0	37.0	35.0	37.0
8	36.22621232616249	37.0	36.0	37.0	35.0	37.0
9	37.994390200562485	39.0	38.0	39.0	35.0	39.0
10	37.9066135090129	39.0	38.0	39.0	35.0	39.0
11	38.02237495015023	39.0	38.0	39.0	35.0	39.0
12	37.98323021882964	39.0	38.0	39.0	35.0	39.0
13	38.00888874225773	39.0	38.0	39.0	35.0	39.0
14	39.39695755780403	40.0	39.0	41.0	37.0	41.0
15	39.40586713712116	40.0	39.0	41.0	37.0	41.0
16	39.298836423874405	40.0	39.0	41.0	36.0	41.0
17	39.350863667171964	40.0	39.0	41.0	37.0	41.0
18	39.37322704117808	40.0	39.0	41.0	37.0	41.0
19	39.413411310240086	40.0	39.0	41.0	37.0	41.0
20	39.386328921086005	40.0	39.0	41.0	37.0	41.0
21	39.357102630042064	40.0	39.0	41.0	37.0	41.0
22	39.340519803599136	40.0	39.0	41.0	37.0	41.0
23	39.30480624139386	40.0	39.0	41.0	36.0	41.0
24	39.25688158357021	40.0	39.0	41.0	36.0	41.0
25	39.20072107801685	40.0	39.0	41.0	36.0	41.0
26	39.1185565011336	40.0	39.0	41.0	36.0	41.0
27	39.05372835767507	40.0	39.0	41.0	36.0	41.0
28	38.985434895476104	40.0	39.0	41.0	35.0	41.0
29	38.86057055341493	40.0	38.0	41.0	35.0	41.0
30	38.817004313850106	40.0	38.0	41.0	35.0	41.0
31	38.75305537853702	40.0	38.0	41.0	35.0	41.0
32	38.67722005844795	40.0	38.0	41.0	35.0	41.0
33	38.53526701244609	40.0	38.0	41.0	35.0	41.0
34	38.54455860451898	40.0	38.0	41.0	35.0	41.0
35	38.51339273052878	40.0	38.0	41.0	35.0	41.0
36	38.483228771811625	40.0	38.0	41.0	35.0	41.0
37	38.449322245702504	40.0	38.0	41.0	34.0	41.0
38	38.3787048725727	40.0	38.0	41.0	34.0	41.0
39	38.1958515729954	40.0	38.0	41.0	34.0	41.0
40	38.191907580695855	40.0	38.0	41.0	34.0	41.0
41	38.18805156709157	40.0	38.0	41.0	34.0	41.0
42	38.054030495036436	40.0	37.0	41.0	34.0	41.0
43	37.2123805703682	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	4.0
12	3.0
13	0.0
14	1.0
15	3.0
16	7.0
17	15.0
18	23.0
19	51.0
20	94.0
21	209.0
22	382.0
23	644.0
24	1169.0
25	1818.0
26	2786.0
27	4064.0
28	5746.0
29	8261.0
30	11151.0
31	15766.0
32	21667.0
33	29810.0
34	43882.0
35	64993.0
36	106488.0
37	197426.0
38	468012.0
39	743213.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.0420769686246	17.179576671985906	11.201540090212891	27.576806269176608
2	19.741261602913948	19.358959443557904	32.67760265001091	28.222176303517237
3	20.723034385199668	20.611440355943277	28.440155097178838	30.225370161678217
4	15.114392562095885	14.455015393377636	32.97146306833803	37.45912897618845
5	16.52083619119391	34.3493715021957	32.88383165739707	16.245960649213316
6	37.13464965668051	33.16142759324439	14.647005743503902	15.056917006571199
7	31.95652463316646	28.46851665025748	19.54967641783166	20.025282298744393
8	29.3874888507262	31.556742496198687	18.679787068405172	20.375981584669944
9	27.7685650964206	13.064257439553717	17.916629767707303	41.25054769631838
10	17.714741814363798	25.506470775155975	31.604899255711814	25.173888154768413
11	38.95517196072677	19.841279488056603	19.810660586875777	21.392887964340844
12	23.86954611675352	23.427163769447198	27.453115169321368	25.25017494447792
13	32.71580392558623	17.777716038342504	22.69178921462229	26.814690821448973
14	24.40777893732155	18.38471115494611	23.210284709476404	33.99722519825594
15	27.09101338144379	25.545829665142666	20.50719717819911	26.855959775214433
16	27.311828330413253	23.788802511560228	21.878159925588545	27.021209232437975
17	25.85080318181897	24.458656090701407	23.320952647203697	26.36958808027593
18	26.389093883107567	23.073107401728667	24.06037885246841	26.477419862695356
19	26.835527880853693	23.455930487569827	23.688610984255863	26.019930647320617
20	26.97635167399726	23.328824425201034	23.757778445335422	25.937045455466283
21	27.490043069044173	23.080110968917474	23.491527130719554	25.938318831318796
22	27.45647225111435	23.04688743531106	23.001914115429205	26.494726198145386
23	26.655576720605712	23.136544671471924	23.453036451541394	26.754842156380974
24	26.47724622053365	23.28645573774477	23.16710569193218	27.0691923497894
25	26.993484367285586	22.999772528768165	23.31163385119214	26.695109252754108
26	26.86053235213936	22.944207037022245	23.54443010931932	26.650830501519078
27	26.422491058875693	23.041736051180447	23.604278774387318	26.931494115556543
28	26.250758960948456	23.051575773677122	23.832791859192415	26.86487340618201
29	26.202254917111915	23.447422021646233	23.696887927297183	26.65343513394467
30	25.858269794772333	23.7561577851595	23.72698590199289	26.658586518075282
31	26.821115581432096	23.51774709713716	23.28524024261283	26.37589707881791
32	26.11468138689152	23.457319624863473	23.613308166796028	26.814690821448973
33	25.84744610002599	23.303646311753663	24.020788439599443	26.828119148620903
34	26.626636360321378	22.800084042806265	23.826482860650426	26.746796736221928
35	26.071270846465023	23.255952598005084	24.379591026404608	26.29318552912529
36	26.239414339716998	23.103957825791767	24.079884655300052	26.576743179191187
37	26.219908536885356	22.487296628853194	24.523366736297174	26.769428097964276
38	25.533848355984958	22.695493580738685	25.130651256503622	26.640006806772735
39	25.604868000122703	22.51426904463819	25.226385968324195	26.654476986914904
40	25.113634324656438	22.729816848035906	25.568171623282172	26.588377204025488
41	25.04788182609043	22.18620112045499	26.233047460454443	26.532869593000136
42	24.886857661468397	22.08363648360731	26.42197013239057	26.607535722533715
43	24.131051212282753	21.68043938412598	26.812433473346797	27.37607593024447
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	29.5
2	40.0
3	120.0
4	200.0
5	200.0
6	291.0
7	382.0
8	351.5
9	321.0
10	462.5
11	604.0
12	604.0
13	1147.0
14	1690.0
15	2729.0
16	3768.0
17	3729.5
18	3691.0
19	3691.0
20	4449.0
21	5207.0
22	5253.0
23	5299.0
24	6482.5
25	7666.0
26	7666.0
27	9940.0
28	12214.0
29	15082.5
30	17951.0
31	21941.5
32	25932.0
33	25932.0
34	32248.0
35	38564.0
36	45410.5
37	52257.0
38	58538.5
39	64820.0
40	64820.0
41	72185.5
42	79551.0
43	87866.0
44	96181.0
45	103763.0
46	111345.0
47	111345.0
48	118007.5
49	124670.0
50	129854.5
51	135039.0
52	137989.5
53	140940.0
54	140940.0
55	139417.0
56	137894.0
57	133583.5
58	129273.0
59	127542.0
60	125811.0
61	125811.0
62	119668.5
63	113526.0
64	102236.5
65	90947.0
66	80677.5
67	70408.0
68	70408.0
69	59849.5
70	49291.0
71	42187.0
72	35083.0
73	27044.0
74	19005.0
75	19005.0
76	15071.5
77	11138.0
78	8926.0
79	6714.0
80	5735.5
81	4757.0
82	4757.0
83	3678.0
84	2599.0
85	2064.0
86	1529.0
87	1160.0
88	791.0
89	791.0
90	603.5
91	416.0
92	260.0
93	104.0
94	68.5
95	33.0
96	33.0
97	24.0
98	15.0
99	10.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1727691.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.61194052825491
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.09559266430516	45.92623505271383
2	8.31726980099406	9.084444874586763
3	2.590581450262253	4.244300402859675
4	1.2567549117221473	2.7453529799024863
5	0.7704420807745401	2.103766854786207
6	0.4966599721640724	1.6274138917553436
7	0.34618665850257946	1.3234147644072711
8	0.2718879470120588	1.1878662714057502
9	0.20287305377132014	0.9971362032610317
>10	1.3945160803615262	14.891157429754987
>50	0.16196544010281727	6.141284043107911
>100	0.09303469001391038	8.759659628122266
>500	0.0019159285829569693	0.6720899249205162
>1k	3.1932143049282825E-4	0.29587767841596846
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2007	0.11616660618131366	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	5.7880720568666504E-5	0.0
10	0.0	0.0	0.0	2.3152288227466601E-4	0.0
11	0.0	0.0	0.0	2.894036028433325E-4	0.0
12	0.0	0.0	0.0	2.894036028433325E-4	0.0
13	0.0	0.0	0.0	3.47284323411999E-4	0.0
14	0.0	0.0	0.0	3.47284323411999E-4	0.0
15	0.0	0.0	0.0	4.6304576454933203E-4	0.0
16	0.0	0.0	0.0	6.366879262553316E-4	0.0
17	0.0	0.0	0.0	6.94568646823998E-4	0.0
18	0.0	0.0	0.0	8.103300879613311E-4	0.0
19	0.0	0.0	0.0	9.260915290986641E-4	0.0
20	0.0	0.0	0.0	0.0012733758525106632	0.0
21	0.0	0.0	0.0	0.0015627794553539956	5.7880720568666504E-5
22	0.0	0.0	0.0	0.0018521830581973281	5.7880720568666504E-5
23	0.0	0.0	0.0	0.0026046324255899925	5.7880720568666504E-5
24	0.0	0.0	0.0	0.003588604675257323	5.7880720568666504E-5
25	0.0	0.0	0.0	0.004919861248336653	5.7880720568666504E-5
26	0.0	0.0	0.0	0.006772044306533981	5.7880720568666504E-5
27	0.0	0.0	0.0	0.01655388608263862	1.1576144113733301E-4
28	0.0	0.0	0.0	0.05284509787919252	1.1576144113733301E-4
29	0.0	0.0	0.0	0.10777390169885703	1.1576144113733301E-4
30	0.0	0.0	0.0	0.18047208673310217	1.1576144113733301E-4
31	0.0	0.0	0.0	0.3794081233276089	1.1576144113733301E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1160	0.0	22.168102	1
GTCTAGA	325	0.0	19.923077	1
TCTAGAC	320	0.0	19.078125	3
ATTCCGC	130	6.9667294E-10	18.5	28
GTTCTAG	340	0.0	18.499998	1
TCTATAC	175	0.0	17.97143	3
TAACGCC	200	0.0	17.575	4
CTAGAAC	370	0.0	17.5	3
TAGAACT	385	0.0	17.2987	4
CTAGACA	350	0.0	16.914286	4
TCGTTTA	190	1.8189894E-12	16.552631	30
CGACGGT	160	6.2937033E-10	16.1875	7
ATACCGT	115	1.2430028E-6	16.086956	6
TACCGTC	115	1.2430028E-6	16.086956	7
TAATACT	150	4.6748028E-9	16.033333	4
GACGTAT	245	0.0	15.857142	26
ACTGATC	340	0.0	15.779411	8
GTAATAC	130	2.5909685E-7	15.653846	3
ACGTATG	250	0.0	15.54	27
ATAACGC	250	0.0	15.54	3
>>END_MODULE