FastQCFastQC Report
Fri 10 Feb 2017
ERR1632267.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632267.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3903
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT521.3323084806559056TruSeq Adapter, Index 3 (95% over 21bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC330.8455034588777863No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC240.6149116064565718No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC240.6149116064565718RNA PCR Primer, Index 3 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT160.4099410709710479No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG100.25621316935690497No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG100.25621316935690497No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)