FastQCFastQC Report
Fri 10 Feb 2017
ERR1632266.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632266.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479345
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8410.17544774640394703No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5960.12433633395571039No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC5930.123710479925732No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA5900.12308462589575359No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5830.12162429982580396No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG5320.11098478131617102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2650.026.5283031
GTGCTAT358.861823E-426.428571
GATATAC358.861823E-426.428573
ATTATAC451.32164E-424.6666683
ATAGGAC551.8996598E-523.5454543
TATACAA855.1648385E-821.7647065
ATATCCC957.1195245E-921.4210533
GTATATC1001.285116E-820.3499981
CTATGCA656.891403E-519.9230774
ATCTCGC1551.8189894E-1219.09677511
CTTATAC5450.019.00917437
TGGACTA500.007029046318.4999985
TATATCC1054.782851E-717.6190472
TCTCGCC1800.017.47222312
TAGCACA650.001578117317.0769234
AGTGTTC650.001578117317.0769238
CGGCCTT1851.8189894E-1217.00000224
ATCCCTT1201.0374424E-716.9583345
TCTTATA9850.016.90355337
TGCTAGA1151.2384262E-616.0869562
Produced by FastQC (version 0.11.5)