##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632266.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	479345
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45073381385015	33.0	31.0	34.0	31.0	34.0
2	32.61821026609227	34.0	31.0	34.0	31.0	34.0
3	32.629340036925385	34.0	31.0	34.0	31.0	34.0
4	36.19687698839041	37.0	37.0	37.0	35.0	37.0
5	36.0462610437159	37.0	35.0	37.0	35.0	37.0
6	36.0753131877875	37.0	35.0	37.0	35.0	37.0
7	36.06857065370453	37.0	35.0	37.0	35.0	37.0
8	36.03921601351845	37.0	35.0	37.0	35.0	37.0
9	37.769047345857366	39.0	37.0	39.0	35.0	39.0
10	37.673683881129456	39.0	37.0	39.0	35.0	39.0
11	37.7941107135779	39.0	37.0	39.0	35.0	39.0
12	37.755170075832645	39.0	37.0	39.0	35.0	39.0
13	37.775026338023764	39.0	37.0	39.0	35.0	39.0
14	39.102383460764166	40.0	38.0	41.0	36.0	41.0
15	39.11923562361139	40.0	38.0	41.0	36.0	41.0
16	39.00593518238429	40.0	38.0	41.0	36.0	41.0
17	39.05544440851579	40.0	38.0	41.0	36.0	41.0
18	39.0695866234132	40.0	38.0	41.0	36.0	41.0
19	39.13522828025744	40.0	39.0	41.0	36.0	41.0
20	39.112021612825835	40.0	39.0	41.0	36.0	41.0
21	39.0841773670321	40.0	39.0	41.0	36.0	41.0
22	39.06580646507213	40.0	39.0	41.0	36.0	41.0
23	39.02113717677247	40.0	39.0	41.0	36.0	41.0
24	38.96300994064818	40.0	38.0	41.0	36.0	41.0
25	38.89186076834013	40.0	38.0	41.0	35.0	41.0
26	38.80650262337148	40.0	38.0	41.0	35.0	41.0
27	38.73418519020747	40.0	38.0	41.0	35.0	41.0
28	38.67646684538276	40.0	38.0	41.0	35.0	41.0
29	38.53063033931719	40.0	38.0	41.0	35.0	41.0
30	38.48920923343312	40.0	38.0	41.0	34.0	41.0
31	38.40668203486007	40.0	38.0	41.0	34.0	41.0
32	38.338044623392335	40.0	38.0	41.0	34.0	41.0
33	38.20065714673148	40.0	38.0	41.0	34.0	41.0
34	38.199881087734305	40.0	38.0	41.0	34.0	41.0
35	38.18348162596877	40.0	38.0	41.0	34.0	41.0
36	38.15890642439162	40.0	38.0	41.0	34.0	41.0
37	38.148794709447266	40.0	38.0	41.0	34.0	41.0
38	38.09131418915395	40.0	38.0	41.0	34.0	41.0
39	37.9216639372477	40.0	38.0	41.0	33.0	41.0
40	37.94106541217703	40.0	37.0	41.0	33.0	41.0
41	37.955005267604754	40.0	37.0	41.0	33.0	41.0
42	37.854065443469736	40.0	37.0	41.0	33.0	41.0
43	36.999013236812736	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	4.0
18	11.0
19	16.0
20	44.0
21	77.0
22	148.0
23	229.0
24	371.0
25	615.0
26	904.0
27	1386.0
28	1999.0
29	2895.0
30	3889.0
31	5616.0
32	7426.0
33	10305.0
34	14624.0
35	21402.0
36	34225.0
37	62771.0
38	143152.0
39	167234.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.188820160844486	18.35233495707684	12.996067550511636	26.462777331567032
2	18.42514264256433	20.935234538797733	34.8085408213291	25.83108199730883
3	19.938040451032137	21.812056034797482	30.72463465771	27.52526885646038
4	15.540790036403843	15.682233047178961	33.78214021216452	34.99483670425268
5	15.49301651211549	35.47695292534605	33.77734199793468	15.252688564603783
6	34.99337637818273	34.15994742826148	15.675974506879179	15.17070168667661
7	29.640655477787398	30.32575702260376	20.65735534948732	19.37623215012152
8	27.496479571081373	33.352178493569355	20.03734262378871	19.113999311560566
9	26.803659159895272	14.541301150528325	19.01886949900385	39.63617019057255
10	16.981923249434125	27.065474762436242	32.78578059643889	23.166821391690746
11	36.21879856888045	21.223336010597794	21.40462506128154	21.153240359240215
12	22.393057192627438	24.990560035047828	28.44485704450865	24.171525727816082
13	31.222188611542833	19.199324077647624	24.17590670602593	25.40258060478361
14	23.351865566554363	20.132681054355423	24.341549405960215	32.17390397313
15	25.88031584766713	27.064848908406265	21.875267291825303	25.179567952101305
16	25.699026796983382	25.863417788857713	23.031011067185432	25.406544346973476
17	24.59105654591161	25.881358937717092	24.60378224452117	24.92380227185013
18	23.96019568369337	24.837851651733097	26.035527647101773	25.16642501747176
19	25.352929518405325	24.948627815039274	25.875517633437294	23.822925033118107
20	25.579905913277496	24.43563612846697	25.697775088923425	24.28668286933211
21	25.469129749971316	25.20126422514056	24.272914080672585	25.056691944215544
22	25.834002649448728	24.891675098311236	24.307127434311404	24.967194817928632
23	25.182279986231208	25.072546912974996	24.53598139127351	25.209191709520283
24	25.91077407712608	24.654059184929437	24.657605691099313	24.77756104684517
25	25.387142872044144	24.189466876675464	25.13158580980296	25.291804441477435
26	25.224212206239766	24.30900499640134	24.882495905871554	25.58428689148734
27	24.954051883299087	24.588761747801687	24.932772846279818	25.52441352261941
28	24.676589930008657	24.913162753340494	25.18061104215127	25.22963627449958
29	24.663864231399096	25.36440350895493	25.06002983237543	24.911702427270548
30	24.4289603521472	25.887408860006882	24.699329293097875	24.984301494748042
31	24.96197936767881	25.002034025597432	25.081308869394697	24.954677737329064
32	24.021320760621265	25.225672532309712	25.223586352209786	25.529420354859234
33	24.23598869290386	24.703918889317713	25.690890694593666	25.369201723184766
34	24.587718657751722	24.27333131669257	25.792070429440173	25.346879596115535
35	24.446275646976602	24.152750106916727	26.035110411081792	25.365863835024875
36	24.116867809197966	24.057620294360014	26.265216076103847	25.56029582033817
37	24.33299606755051	22.741866505335405	26.681826242059476	26.24331118505461
38	23.451376357320928	23.054376284304624	27.82505293682004	25.669194421554415
39	23.343312228144654	22.47045447433477	28.55625906184481	25.629974235675768
40	22.70807038771657	21.982705566971596	29.68237907978596	25.626844965525876
41	21.916156421783892	22.123314105706747	30.333893124993484	25.62663634751588
42	21.892999822674692	21.387935620482114	30.553567889515904	26.165496667327293
43	20.729745798954823	21.49328771552848	31.151467106155273	26.625499379361422
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19.0
1	17.0
2	15.0
3	43.0
4	71.0
5	71.0
6	107.0
7	143.0
8	158.0
9	173.0
10	270.5
11	368.0
12	368.0
13	575.5
14	783.0
15	1414.5
16	2046.0
17	2046.0
18	2046.0
19	2046.0
20	2290.5
21	2535.0
22	2871.0
23	3207.0
24	3902.0
25	4597.0
26	4597.0
27	5355.5
28	6114.0
29	8082.0
30	10050.0
31	11228.0
32	12406.0
33	12406.0
34	14168.0
35	15930.0
36	17241.5
37	18553.0
38	21300.0
39	24047.0
40	24047.0
41	26665.0
42	29283.0
43	29018.5
44	28754.0
45	30182.0
46	31610.0
47	31610.0
48	33062.5
49	34515.0
50	36692.5
51	38870.0
52	40434.5
53	41999.0
54	41999.0
55	38623.0
56	35247.0
57	33246.0
58	31245.0
59	28510.0
60	25775.0
61	25775.0
62	23910.5
63	22046.0
64	19557.0
65	17068.0
66	14931.0
67	12794.0
68	12794.0
69	10962.5
70	9131.0
71	7937.5
72	6744.0
73	5582.5
74	4421.0
75	4421.0
76	3606.5
77	2792.0
78	2322.5
79	1853.0
80	1444.0
81	1035.0
82	1035.0
83	783.5
84	532.0
85	412.5
86	293.0
87	225.0
88	157.0
89	157.0
90	107.5
91	58.0
92	36.0
93	14.0
94	9.0
95	4.0
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	479345.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.68243180483219
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.49733968546796	68.15428923502893
2	4.524616662731637	6.667695173894626
3	1.0486122813705807	2.31792908735392
4	0.49847158206992315	1.4691439341005574
5	0.26502428954331647	0.9763817070449762
6	0.18661895780295482	0.8250323179083046
7	0.13132783528691244	0.6773587987322913
8	0.10524149879069164	0.6203559646146773
9	0.07582283485988875	0.5028129772931527
>10	0.5474703407538956	7.991492022993945
>50	0.07707629241781346	3.960748596234055
>100	0.040671252774061535	5.054040836451137
>500	0.001706486130380198	0.7827193483495025
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	841	0.17544774640394703	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	596	0.12433633395571039	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	593	0.123710479925732	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	590	0.12308462589575359	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	583	0.12162429982580396	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	532	0.11098478131617102	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.086180099928027E-4	0.0	2.086180099928027E-4	0.0
3	0.0	2.086180099928027E-4	0.0	2.086180099928027E-4	0.0
4	0.0	2.086180099928027E-4	0.0	2.086180099928027E-4	0.0
5	0.0	2.086180099928027E-4	0.0	4.172360199856054E-4	0.0
6	0.0	2.086180099928027E-4	0.0	4.172360199856054E-4	0.0
7	0.0	2.086180099928027E-4	0.0	6.25854029978408E-4	0.0
8	0.0	2.086180099928027E-4	0.0	6.25854029978408E-4	0.0
9	0.0	2.086180099928027E-4	0.0	8.344720399712108E-4	0.0
10	0.0	2.086180099928027E-4	0.0	0.0010430900499640134	0.0
11	0.0	2.086180099928027E-4	0.0	0.0010430900499640134	0.0
12	0.0	2.086180099928027E-4	0.0	0.001251708059956816	0.0
13	0.0	2.086180099928027E-4	0.0	0.001251708059956816	0.0
14	0.0	2.086180099928027E-4	0.0	0.001251708059956816	0.0
15	0.0	2.086180099928027E-4	0.0	0.0014603260699496187	0.0
16	0.0	2.086180099928027E-4	0.0	0.0016689440799424215	0.0
17	0.0	2.086180099928027E-4	0.0	0.0018775620899352241	0.0
18	0.0	2.086180099928027E-4	0.0	0.0018775620899352241	0.0
19	0.0	2.086180099928027E-4	0.0	0.0018775620899352241	0.0
20	0.0	2.086180099928027E-4	0.0	0.002503416119913632	0.0
21	0.0	2.086180099928027E-4	0.0	0.002712034129906435	0.0
22	0.0	2.086180099928027E-4	0.0	0.00312927014989204	0.0
23	0.0	2.086180099928027E-4	0.0	0.0037551241798704483	0.0
24	0.0	2.086180099928027E-4	0.0	0.005632686269805672	0.0
25	0.0	2.086180099928027E-4	0.0	0.007301630349748094	0.0
26	0.0	2.086180099928027E-4	0.0	0.01022228248964733	0.0
27	0.0	2.086180099928027E-4	0.0	0.03400473562882684	0.0
28	0.0	2.086180099928027E-4	0.0	0.1260052780356528	0.0
29	0.0	2.086180099928027E-4	0.0	0.25326226413126246	0.0
30	0.0	2.086180099928027E-4	0.0	0.4182791100355694	0.0
31	0.0	2.086180099928027E-4	0.0	0.9830080630860862	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	265	0.0	26.528303	1
GTGCTAT	35	8.861823E-4	26.42857	1
GATATAC	35	8.861823E-4	26.42857	3
ATTATAC	45	1.32164E-4	24.666668	3
ATAGGAC	55	1.8996598E-5	23.545454	3
TATACAA	85	5.1648385E-8	21.764706	5
ATATCCC	95	7.1195245E-9	21.421053	3
GTATATC	100	1.285116E-8	20.349998	1
CTATGCA	65	6.891403E-5	19.923077	4
ATCTCGC	155	1.8189894E-12	19.096775	11
CTTATAC	545	0.0	19.009174	37
TGGACTA	50	0.0070290463	18.499998	5
TATATCC	105	4.782851E-7	17.619047	2
TCTCGCC	180	0.0	17.472223	12
TAGCACA	65	0.0015781173	17.076923	4
AGTGTTC	65	0.0015781173	17.076923	8
CGGCCTT	185	1.8189894E-12	17.000002	24
ATCCCTT	120	1.0374424E-7	16.958334	5
TCTTATA	985	0.0	16.903553	37
TGCTAGA	115	1.2384262E-6	16.086956	2
>>END_MODULE