FastQCFastQC Report
Fri 10 Feb 2017
ERR1632265.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632265.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1472264
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG15160.1029706628702461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5800.025.8362081
TAATCCG450.003826312320.5555535
ACGCTAA609.238494E-418.534
TACGTTA500.007035797518.519
CTTATAC9450.017.81481637
GTATCAA8950.016.5363142
CGCTAAG700.00259295815.85714235
GTATTAC1201.9362997E-615.4166671
GGCTATA855.365423E-415.2352931
TTTCGAG750.00410513414.833
TATAAGG750.00410513414.82
CTCTTAT23950.014.52192137
GTACTAT1304.445714E-614.2307691
GTACTGG2252.7284841E-1113.97777751
TAACGCT800.0062997313.87532
TAGTGCG800.0062997313.8759
GTATTAA2700.013.7037041
TCTTATA14500.013.65172537
TAATACT1652.5960617E-713.4545454
GTCTAAG1102.4570953E-413.4545451
Produced by FastQC (version 0.11.5)