##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632265.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1472264
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.840023935924535	34.0	31.0	34.0	31.0	34.0
2	32.99953473018426	34.0	33.0	34.0	31.0	34.0
3	32.993025707345964	34.0	33.0	34.0	31.0	34.0
4	36.42589032945178	37.0	37.0	37.0	35.0	37.0
5	36.35221536354893	37.0	37.0	37.0	35.0	37.0
6	36.38865448044644	37.0	37.0	37.0	35.0	37.0
7	36.38523661517228	37.0	37.0	37.0	35.0	37.0
8	36.36817513706781	37.0	37.0	37.0	35.0	37.0
9	38.16860019670385	39.0	39.0	39.0	37.0	39.0
10	38.10859465421962	39.0	38.0	39.0	37.0	39.0
11	38.20814201800764	39.0	39.0	39.0	37.0	39.0
12	38.181009655876935	39.0	39.0	39.0	37.0	39.0
13	38.19463900496107	39.0	39.0	39.0	37.0	39.0
14	39.643077600213005	41.0	39.0	41.0	37.0	41.0
15	39.67268098656219	41.0	39.0	41.0	38.0	41.0
16	39.55854792347025	41.0	39.0	41.0	37.0	41.0
17	39.60160881472345	41.0	39.0	41.0	37.0	41.0
18	39.61553362712122	41.0	39.0	41.0	37.0	41.0
19	39.66904916509539	41.0	40.0	41.0	37.0	41.0
20	39.65183486113904	41.0	40.0	41.0	37.0	41.0
21	39.627714187129484	41.0	39.0	41.0	37.0	41.0
22	39.61660748344047	41.0	39.0	41.0	37.0	41.0
23	39.5827093510403	41.0	39.0	41.0	37.0	41.0
24	39.539693967929665	41.0	39.0	41.0	37.0	41.0
25	39.48639102769612	41.0	39.0	41.0	37.0	41.0
26	39.414744230654286	41.0	39.0	41.0	37.0	41.0
27	39.35610189476887	41.0	39.0	41.0	37.0	41.0
28	39.30116134062913	40.0	39.0	41.0	36.0	41.0
29	39.17365499665821	40.0	39.0	41.0	36.0	41.0
30	39.14643841050246	40.0	39.0	41.0	36.0	41.0
31	39.08201314438171	40.0	39.0	41.0	36.0	41.0
32	39.02608499562579	40.0	39.0	41.0	35.0	41.0
33	38.90152988866127	40.0	38.0	41.0	35.0	41.0
34	38.906510652980714	40.0	38.0	41.0	35.0	41.0
35	38.89274410024289	40.0	38.0	41.0	35.0	41.0
36	38.86721267381394	40.0	38.0	41.0	35.0	41.0
37	38.840253514315364	40.0	38.0	41.0	35.0	41.0
38	38.7736628756799	40.0	38.0	41.0	35.0	41.0
39	38.58882510201975	40.0	38.0	41.0	35.0	41.0
40	38.602748555965505	40.0	38.0	41.0	35.0	41.0
41	38.609016453570824	40.0	38.0	41.0	35.0	41.0
42	38.490957464150455	40.0	38.0	41.0	35.0	41.0
43	37.69122249813892	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	1.0
14	0.0
15	2.0
16	2.0
17	7.0
18	15.0
19	32.0
20	62.0
21	152.0
22	245.0
23	468.0
24	649.0
25	1035.0
26	1685.0
27	2422.0
28	3546.0
29	4933.0
30	7105.0
31	9822.0
32	13948.0
33	19910.0
34	29829.0
35	45800.0
36	75993.0
37	144206.0
38	354107.0
39	756286.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.49328653013318	17.73866643482419	11.92000891144523	26.8480381235974
2	19.737424809680874	20.07398129683263	33.347823488178754	26.840770405307744
3	21.084601674699645	21.060557074003032	28.702189281270208	29.152651970027115
4	15.709274966989618	15.199923383306254	32.51848853194807	36.57231311775605
5	16.594238533306527	34.424668401862704	32.745621709149994	16.235471355680776
6	36.656469220194204	32.43046084126217	15.165011166475578	15.748058772068052
7	30.686751832551774	28.66666576103199	20.07506805844604	20.5715143479702
8	27.619706791716702	32.15992512212484	19.62528459569751	20.59508349046095
9	27.01268250802845	13.790461493319134	18.73291746588927	40.463938532763144
10	17.68752071639326	26.022506833013644	32.4294420022496	23.860530448343503
11	36.98372031103118	20.74335852809007	20.26946254204409	22.003458618834664
12	22.48815429841387	24.248708112132064	27.588122782327083	25.675014807126985
13	31.672580461112954	18.83249199871762	23.232721848798857	26.26220569137057
14	24.153820238761526	19.91198589383426	23.208405557698892	32.72578830970532
15	26.45048714089321	26.506455363983633	21.070473773725364	25.97258372139779
16	26.94645797221151	24.628395450815887	22.51980623040433	25.905340346568277
17	25.317198545912962	25.112955285193415	23.765982187977155	25.803863980916464
18	24.77660256584417	24.08664478653285	24.993751120722916	26.14300152690007
19	26.37529682176566	24.34644873473779	24.561355843788885	24.716898599707662
20	26.479082555845963	23.968934919280784	24.556601261730233	24.995381263143024
21	25.784845652681852	24.509734667152085	23.581504404101437	26.12391527606462
22	26.578996701678502	23.782759070384117	23.616416620932114	26.021827607005264
23	25.484356066575014	24.37164801964865	23.860190835339314	26.283805078437016
24	26.42460862997397	24.3505920133889	23.53857732037189	25.68622203626523
25	26.30404601348671	23.818622203626525	23.92125325349258	25.956078529394183
26	26.202297957431547	24.17175180538273	23.47866958643287	26.14728065075285
27	26.052868235588182	23.93932066531546	23.584764688941657	26.4230464101547
28	25.60879027131004	24.150492031320468	24.187645693978794	26.0530720033907
29	25.709927023957658	24.536564094483055	24.21556188292317	25.537946998636112
30	25.64784576679183	24.672477218759678	23.624771100835176	26.054905913613318
31	25.72731520977216	23.971991436318486	24.123526758787825	26.177166595121527
32	25.016708959806124	24.236142430977054	24.31167236310879	26.435476246108035
33	25.367461270533003	23.77820825612798	24.433933044617	26.42039742872202
34	25.916140040101503	23.55535420277885	24.487795667081446	26.0407100900382
35	25.814188216243828	23.50305380013367	24.417292007411714	26.265465976210788
36	25.113634511201795	23.726043698684478	24.524270103731396	26.636051686382334
37	25.51811359919145	22.459558883461117	25.463911363722815	26.55841615362462
38	24.74535816945874	22.963816272081637	25.918041872924963	26.372783685534664
39	24.497168985997074	22.77573859036151	26.896466937994816	25.830625485646596
40	24.435902800041298	22.063162584971174	27.265014970141227	26.235919644846305
41	23.54849402009422	22.595132394733554	27.705764726978316	26.150608858193912
42	23.669939630392374	22.420027929773465	27.35990284351176	26.550129596322396
43	23.079420538707733	22.28092244325746	27.680565442067458	26.959091575967353
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	45.0
1	51.0
2	57.0
3	127.5
4	198.0
5	198.0
6	268.0
7	338.0
8	339.5
9	341.0
10	447.5
11	554.0
12	554.0
13	959.0
14	1364.0
15	2025.0
16	2686.0
17	3005.5
18	3325.0
19	3325.0
20	4372.5
21	5420.0
22	6544.5
23	7669.0
24	9420.5
25	11172.0
26	11172.0
27	13030.5
28	14889.0
29	19963.5
30	25038.0
31	28207.0
32	31376.0
33	31376.0
34	36779.5
35	42183.0
36	46676.0
37	51169.0
38	58089.0
39	65009.0
40	65009.0
41	71671.5
42	78334.0
43	79871.5
44	81409.0
45	86781.5
46	92154.0
47	92154.0
48	96423.5
49	100693.0
50	107193.5
51	113694.0
52	122265.5
53	130837.0
54	130837.0
55	121455.5
56	112074.0
57	105727.0
58	99380.0
59	94972.5
60	90565.0
61	90565.0
62	85698.0
63	80831.0
64	71637.5
65	62444.0
66	56107.5
67	49771.0
68	49771.0
69	43976.5
70	38182.0
71	35175.5
72	32169.0
73	27030.5
74	21892.0
75	21892.0
76	16090.5
77	10289.0
78	8489.5
79	6690.0
80	5301.5
81	3913.0
82	3913.0
83	3003.0
84	2093.0
85	1611.0
86	1129.0
87	835.5
88	542.0
89	542.0
90	398.5
91	255.0
92	163.0
93	71.0
94	40.5
95	10.0
96	10.0
97	9.0
98	8.0
99	5.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1472264.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.65629622971247
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.52999877268974	54.58431829545041
2	6.6951943817699355	8.256017762358276
3	1.7475499623346717	3.2324237446178814
4	0.793993072516514	1.9581868833367104
5	0.46482828613175287	1.4329795252844453
6	0.29842588476984455	1.1039900852390145
7	0.20538558664557494	0.8864320200072993
8	0.16056517429636025	0.7919883160473441
9	0.11668972912475342	0.6475190855292633
>10	0.7980952303150121	9.61248267106536
>50	0.09820819627819385	4.231094705941861
>100	0.08419706905411078	10.246047517046524
>500	0.006093160871673145	2.449239973240016
>1k	7.754932018493094E-4	0.5672794148355506
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1516	0.1029706628702461	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	6.792260083789321E-5	6.792260083789321E-5	0.0	0.0	0.0
3	6.792260083789321E-5	6.792260083789321E-5	0.0	0.0	0.0
4	6.792260083789321E-5	6.792260083789321E-5	0.0	0.0	0.0
5	6.792260083789321E-5	6.792260083789321E-5	0.0	0.0	0.0
6	6.792260083789321E-5	6.792260083789321E-5	0.0	0.0	0.0
7	6.792260083789321E-5	6.792260083789321E-5	0.0	0.0	0.0
8	6.792260083789321E-5	6.792260083789321E-5	0.0	0.0	0.0
9	6.792260083789321E-5	6.792260083789321E-5	0.0	6.792260083789321E-5	0.0
10	6.792260083789321E-5	6.792260083789321E-5	0.0	1.3584520167578642E-4	0.0
11	6.792260083789321E-5	1.3584520167578642E-4	0.0	2.037678025136796E-4	0.0
12	6.792260083789321E-5	1.3584520167578642E-4	0.0	2.037678025136796E-4	0.0
13	6.792260083789321E-5	1.3584520167578642E-4	0.0	2.037678025136796E-4	0.0
14	6.792260083789321E-5	1.3584520167578642E-4	0.0	2.037678025136796E-4	0.0
15	6.792260083789321E-5	1.3584520167578642E-4	0.0	2.037678025136796E-4	0.0
16	6.792260083789321E-5	2.7169040335157284E-4	0.0	3.39613004189466E-4	0.0
17	6.792260083789321E-5	2.7169040335157284E-4	0.0	5.433808067031457E-4	0.0
18	6.792260083789321E-5	2.7169040335157284E-4	0.0	5.433808067031457E-4	0.0
19	6.792260083789321E-5	2.7169040335157284E-4	0.0	6.79226008378932E-4	0.0
20	6.792260083789321E-5	2.7169040335157284E-4	0.0	8.150712100547184E-4	6.792260083789321E-5
21	6.792260083789321E-5	2.7169040335157284E-4	0.0	0.0010867616134062914	6.792260083789321E-5
22	6.792260083789321E-5	2.7169040335157284E-4	0.0	0.0014263746175957573	6.792260083789321E-5
23	6.792260083789321E-5	5.433808067031457E-4	0.0	0.0025131362310020487	6.792260083789321E-5
24	6.792260083789321E-5	5.433808067031457E-4	0.0	0.0038715882477599124	6.792260083789321E-5
25	6.792260083789321E-5	5.433808067031457E-4	0.0	0.0050941950628419904	6.792260083789321E-5
26	6.792260083789321E-5	5.433808067031457E-4	0.0	0.008354479903060864	6.792260083789321E-5
27	6.792260083789321E-5	5.433808067031457E-4	0.0	0.027916188944374108	6.792260083789321E-5
28	6.792260083789321E-5	5.433808067031457E-4	0.0	0.07906190737530769	6.792260083789321E-5
29	6.792260083789321E-5	7.471486092168252E-4	0.0	0.14616943700314616	6.792260083789321E-5
30	6.792260083789321E-5	7.471486092168252E-4	0.0	0.22720109980275277	6.792260083789321E-5
31	6.792260083789321E-5	7.471486092168252E-4	0.0	0.5008612585786245	6.792260083789321E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	580	0.0	25.836208	1
TAATCCG	45	0.0038263123	20.555553	5
ACGCTAA	60	9.238494E-4	18.5	34
TACGTTA	50	0.0070357975	18.5	19
CTTATAC	945	0.0	17.814816	37
GTATCAA	895	0.0	16.536314	2
CGCTAAG	70	0.002592958	15.857142	35
GTATTAC	120	1.9362997E-6	15.416667	1
GGCTATA	85	5.365423E-4	15.235293	1
TTTCGAG	75	0.004105134	14.8	33
TATAAGG	75	0.004105134	14.8	2
CTCTTAT	2395	0.0	14.521921	37
GTACTAT	130	4.445714E-6	14.230769	1
GTACTGG	225	2.7284841E-11	13.9777775	1
TAACGCT	80	0.00629973	13.875	32
TAGTGCG	80	0.00629973	13.875	9
GTATTAA	270	0.0	13.703704	1
TCTTATA	1450	0.0	13.651725	37
TAATACT	165	2.5960617E-7	13.454545	4
GTCTAAG	110	2.4570953E-4	13.454545	1
>>END_MODULE