FastQCFastQC Report
Fri 10 Feb 2017
ERR1632264.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632264.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences305649
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGTA200.00183982337.09
GGTATCA1950.023.7179471
TAAGTCT400.001927962523.1255
ATCATAT502.6958165E-422.21
ATATCCA450.0038194820.5555554
GCCCCTA902.1417145E-618.527
CTATAGT500.00702335718.51
ACTAGAC500.00702335718.53
CTCTAAA500.00702335718.51
CCATTAT500.00702335718.59
GTATCAA2600.017.7884622
TCTTATA2500.017.7637
GTCTTGC650.001576348617.0769231
GTGTAGT803.372413E-416.18751
ACTGTGC855.348164E-415.2352948
CTCTTAT4250.015.23529337
TCTCTTA7450.014.89932837
CCGCTAC1001.0891973E-414.81
TACACCA1252.9413932E-614.7999995
CCCTACT1152.1992577E-514.47826129