##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632264.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	305649
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55944891035141	34.0	31.0	34.0	31.0	34.0
2	32.72721324133238	34.0	31.0	34.0	31.0	34.0
3	32.727795608688396	34.0	31.0	34.0	31.0	34.0
4	36.25543024842221	37.0	37.0	37.0	35.0	37.0
5	36.12710658304133	37.0	35.0	37.0	35.0	37.0
6	36.16541523119657	37.0	36.0	37.0	35.0	37.0
7	36.161993005048274	37.0	36.0	37.0	35.0	37.0
8	36.133191994739065	37.0	35.0	37.0	35.0	37.0
9	37.87721536795475	39.0	38.0	39.0	35.0	39.0
10	37.78819495565175	39.0	37.0	39.0	35.0	39.0
11	37.90577099876002	39.0	38.0	39.0	35.0	39.0
12	37.86895752971546	39.0	38.0	39.0	35.0	39.0
13	37.88880055226747	39.0	38.0	39.0	35.0	39.0
14	39.25747834934844	40.0	39.0	41.0	36.0	41.0
15	39.271867403459524	40.0	39.0	41.0	36.0	41.0
16	39.14809143821835	40.0	39.0	41.0	36.0	41.0
17	39.197373457789816	40.0	39.0	41.0	36.0	41.0
18	39.196725655899414	40.0	39.0	41.0	36.0	41.0
19	39.25530592280688	40.0	39.0	41.0	36.0	41.0
20	39.22033443590524	40.0	39.0	41.0	36.0	41.0
21	39.20111304142988	40.0	39.0	41.0	36.0	41.0
22	39.17764167394626	40.0	39.0	41.0	36.0	41.0
23	39.13501761824838	40.0	39.0	41.0	36.0	41.0
24	39.085307002476696	40.0	39.0	41.0	36.0	41.0
25	39.016839577423774	40.0	39.0	41.0	36.0	41.0
26	38.93244211497502	40.0	38.0	41.0	35.0	41.0
27	38.861102113862636	40.0	38.0	41.0	35.0	41.0
28	38.797133313048626	40.0	38.0	41.0	35.0	41.0
29	38.656720617440264	40.0	38.0	41.0	35.0	41.0
30	38.618258198129226	40.0	38.0	41.0	35.0	41.0
31	38.550719943464564	40.0	38.0	41.0	35.0	41.0
32	38.48289050512189	40.0	38.0	41.0	34.0	41.0
33	38.337141623234494	40.0	38.0	41.0	34.0	41.0
34	38.3375505890744	40.0	38.0	41.0	34.0	41.0
35	38.31257095557322	40.0	38.0	41.0	34.0	41.0
36	38.2863742397325	40.0	38.0	41.0	34.0	41.0
37	38.266684988336294	40.0	38.0	41.0	34.0	41.0
38	38.18798033037896	40.0	38.0	41.0	34.0	41.0
39	37.99950269753868	40.0	38.0	41.0	33.0	41.0
40	38.015635581991106	40.0	37.0	41.0	33.0	41.0
41	38.02337321568204	40.0	37.0	41.0	33.0	41.0
42	37.914097543260404	40.0	37.0	41.0	33.0	41.0
43	37.03795857339628	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	0.0
15	1.0
16	1.0
17	3.0
18	5.0
19	15.0
20	33.0
21	56.0
22	92.0
23	156.0
24	244.0
25	391.0
26	601.0
27	835.0
28	1204.0
29	1648.0
30	2270.0
31	3170.0
32	4255.0
33	6116.0
34	8647.0
35	12773.0
36	20257.0
37	37281.0
38	87353.0
39	118240.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.71304666463819	18.049789137212947	12.460371210113562	26.776792988035297
2	20.271945924900788	20.093636818703807	33.09449728283096	26.539919973564448
3	21.201443485828516	21.046036466666013	28.706457407025702	29.046062640479764
4	16.33998475375349	15.413758919544968	32.04689038733973	36.19936593936182
5	16.770543990001602	34.24189184325812	32.82425265582417	16.163311510916117
6	36.47975291919817	32.463054026023315	15.010682187738222	16.046510867040297
7	30.97409119611057	28.341659877833724	20.130280157958964	20.553968768096738
8	27.894087662645717	32.01613615617915	19.386616674682397	20.703159506492742
9	27.16383825891791	13.956859011480487	18.8196264342432	40.0596762953584
10	18.219591753939977	25.76779246783075	32.08516958995449	23.927446188274786
11	37.19985997009642	20.530412335718424	19.961459059247698	22.308268634937463
12	22.71559861147918	24.195727779250053	27.5132586725296	25.575414936741165
13	31.861710655032404	18.58798818252309	23.23645750517751	26.313843657266993
14	24.485929939244038	19.91532771250683	22.871005630641683	32.727736717607456
15	27.237452110100147	26.040000130869068	20.500639622573605	26.221908136457177
16	26.941360842011587	24.721494263027196	22.333133758003463	26.00401113695775
17	25.45665125683382	25.078439648093077	23.538110708688727	25.92679838638438
18	25.056519079074363	23.943150476527	24.887697980363097	26.112632464035546
19	26.30893606718818	24.163010512057948	24.417550850812532	25.11050256994134
20	26.477102820555604	23.727543685731018	24.33641202817611	25.458941465537265
21	26.542537354939817	23.98502857853289	23.572136666568515	25.900297399958777
22	26.718229079761425	23.756662053531993	23.262958491603115	26.262150375103467
23	25.96049717159225	23.98077533379792	23.631354920186226	26.4273725744236
24	26.464343086350684	24.216666830253004	23.42948938161093	25.889500701785384
25	26.501640770949685	23.449119741926197	23.665380878066017	26.3838586090581
26	26.254298230977362	24.107063985159446	23.43766869840896	26.20096908545423
27	26.329220772847282	23.560685623051278	23.533203118609908	26.57689048549153
28	25.910439752788328	23.66930695012907	24.103137913096397	26.317115383986206
29	25.53811725214216	24.063877192465867	24.169881138168293	26.22812441722368
30	25.41640901818753	24.689758513850855	24.075001063311184	25.81883140465043
31	26.340017471020683	24.025270817179184	23.76844026972115	25.866271442078986
32	25.4501078033954	23.810318371727046	24.21241358551803	26.527160239359524
33	25.49133156005745	23.732451275809836	24.397593317825347	26.378623846307363
34	26.24350153280397	23.21879018089377	24.20063536932887	26.33707291697339
35	25.730167610559825	23.367653746617854	24.7587919476262	26.143386695196124
36	25.236464048630946	23.71609264221378	24.654751037955304	26.39269227119997
37	25.842060664356826	22.525184116421123	25.134713347663496	26.498041871558552
38	24.929248909697073	22.597489276915677	26.16825181826212	26.30500999512513
39	24.763699537705016	22.51733197229502	26.575908967475765	26.143059522524204
40	24.194746261234293	22.084155354671537	27.42557639645476	26.295521987639415
41	23.664726532722174	22.228438502988723	27.684042807272398	26.422792157016712
42	23.946749375918127	22.009559985473533	27.474325124570996	26.56936551403734
43	23.192616367140083	22.034097935867614	27.940546182058508	26.832739514933795
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	4.5
2	6.0
3	16.0
4	26.0
5	26.0
6	34.0
7	42.0
8	36.5
9	31.0
10	52.0
11	73.0
12	73.0
13	127.5
14	182.0
15	278.5
16	375.0
17	403.0
18	431.0
19	431.0
20	620.5
21	810.0
22	988.5
23	1167.0
24	1492.0
25	1817.0
26	1817.0
27	2208.5
28	2600.0
29	3515.0
30	4430.0
31	5237.5
32	6045.0
33	6045.0
34	7066.0
35	8087.0
36	9248.5
37	10410.0
38	11846.0
39	13282.0
40	13282.0
41	14712.5
42	16143.0
43	16679.5
44	17216.0
45	18541.5
46	19867.0
47	19867.0
48	21020.5
49	22174.0
50	23363.0
51	24552.0
52	26009.5
53	27467.0
54	27467.0
55	25843.5
56	24220.0
57	23529.0
58	22838.0
59	21170.5
60	19503.0
61	19503.0
62	18150.0
63	16797.0
64	15015.0
65	13233.0
66	11667.5
67	10102.0
68	10102.0
69	8779.5
70	7457.0
71	6441.5
72	5426.0
73	4462.0
74	3498.0
75	3498.0
76	2846.5
77	2195.0
78	1784.5
79	1374.0
80	1118.0
81	862.0
82	862.0
83	659.5
84	457.0
85	354.5
86	252.0
87	182.5
88	113.0
89	113.0
90	87.5
91	62.0
92	39.0
93	16.0
94	11.0
95	6.0
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	305649.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.61936216583544
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.63784528423012	66.72700357910698
2	5.231886517408415	7.703362966712833
3	1.4404764999848825	3.1814088343128635
4	0.6847321402600756	2.0163817368157653
5	0.41234935312320764	1.5178448183212698
6	0.28466134360721346	1.2573951929779663
7	0.20478663814255832	1.0553383176098625
8	0.14390668649190255	0.8475454776746171
9	0.11742263362600928	0.7780121452241446
>10	0.7772337750130704	11.19325548128714
>50	0.05309790514065412	2.608869521316346
>100	0.011601222971905554	1.1135819286402888
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	3.271726719210598E-4	3.271726719210598E-4
17	0.0	0.0	0.0	6.543453438421196E-4	3.271726719210598E-4
18	0.0	0.0	0.0	6.543453438421196E-4	3.271726719210598E-4
19	0.0	0.0	0.0	6.543453438421196E-4	3.271726719210598E-4
20	0.0	0.0	0.0	6.543453438421196E-4	3.271726719210598E-4
21	0.0	0.0	0.0	9.815180157631794E-4	3.271726719210598E-4
22	0.0	0.0	0.0	0.0022902087034474185	3.271726719210598E-4
23	0.0	0.0	0.0	0.0026173813753684783	3.271726719210598E-4
24	0.0	3.271726719210598E-4	0.0	0.0052347627507369566	3.271726719210598E-4
25	0.0	3.271726719210598E-4	0.0	0.006216280766500136	3.271726719210598E-4
26	0.0	3.271726719210598E-4	0.0	0.007852144126105435	3.271726719210598E-4
27	0.0	3.271726719210598E-4	0.0	0.024865123066000545	3.271726719210598E-4
28	0.0	3.271726719210598E-4	0.0	0.07361385118223845	3.271726719210598E-4
29	0.0	3.271726719210598E-4	0.0	0.12694299670537118	3.271726719210598E-4
30	0.0	3.271726719210598E-4	0.0	0.21168071873292568	3.271726719210598E-4
31	0.0	3.271726719210598E-4	0.0	0.45738739534564155	3.271726719210598E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATCGTA	20	0.001839823	37.0	9
GGTATCA	195	0.0	23.717947	1
TAAGTCT	40	0.0019279625	23.125	5
ATCATAT	50	2.6958165E-4	22.2	1
ATATCCA	45	0.00381948	20.555555	4
GCCCCTA	90	2.1417145E-6	18.5	27
CTATAGT	50	0.007023357	18.5	1
ACTAGAC	50	0.007023357	18.5	3
CTCTAAA	50	0.007023357	18.5	1
CCATTAT	50	0.007023357	18.5	9
GTATCAA	260	0.0	17.788462	2
TCTTATA	250	0.0	17.76	37
GTCTTGC	65	0.0015763486	17.076923	1
GTGTAGT	80	3.372413E-4	16.1875	1
ACTGTGC	85	5.348164E-4	15.235294	8
CTCTTAT	425	0.0	15.235293	37
TCTCTTA	745	0.0	14.899328	37
CCGCTAC	100	1.0891973E-4	14.8	1
TACACCA	125	2.9413932E-6	14.799999	5
CCCTACT	115	2.1992577E-5	14.478261	29
>>END_MODULE