FastQCFastQC Report
Fri 10 Feb 2017
ERR1632262.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632262.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1582737
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT55770.35236429046645146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT37760.23857406505313267No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36950.23345634808562638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16000.021.0437491
CTTATAC15950.019.83385737
AATAACG1005.880609E-616.652
ATTGCCG3200.016.187511
GTTACAC700.00259307315.8571423
CGAATTA1059.347472E-615.85714215
GTATTAA4450.015.3820221
TTATACT1601.0972144E-815.0312514
TCTTATA25800.014.91472937
CGTTTAG1252.960267E-614.80000126
CTTAACG1001.0937778E-414.79999930
GTAGCAC1001.0937778E-414.7999993
GAGTTAG1001.0937778E-414.7999991
GCTTAGA1405.998445E-714.5357141
CATTAGA908.2764414E-414.3888892
ATACCGT908.2764414E-414.3888896
TACCGTC908.2764414E-414.3888897
TAACGCC1304.4462195E-614.2307694
CCCTATA1051.6562537E-414.0952382
TATACTG1458.9153036E-714.0344835
Produced by FastQC (version 0.11.5)