FastQCFastQC Report
Fri 10 Feb 2017
ERR1632261.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632261.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740157
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8370.1130841159375646No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGAAC250.00549509529.59999829
GGTATCA4600.023.7282621
AGCGTTC500.00703257418.49999827
CTTATAC5350.017.98130837
ATTGTAC650.001579215517.0769233
CGATAAC650.001579215517.07692310
GTATCAA6700.016.2910442
GGACCAT803.38053E-416.18756
ACCGTCC700.00259132415.8571438
CGCAAGA1101.4504003E-515.1363642
CCGATCG750.004102570514.818
GGACTAG750.004102570514.81
AGCGATA750.004102570514.821
GCGATAA750.004102570514.822
ATACCTT750.004102570514.86
CGATAAA750.004102570514.823
CTACACT2006.184564E-1114.7999994
TCTTATA8700.014.67241337
CTCTAGA1152.2076325E-514.4782611
TTTACAC908.269105E-414.3888893