##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632261.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	740157
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25370428165916	33.0	31.0	34.0	30.0	34.0
2	32.432383940164044	34.0	31.0	34.0	31.0	34.0
3	32.44634449177674	34.0	31.0	34.0	31.0	34.0
4	36.079076466209195	37.0	35.0	37.0	35.0	37.0
5	35.82867959095165	37.0	35.0	37.0	35.0	37.0
6	35.839962332315984	37.0	35.0	37.0	35.0	37.0
7	35.83319890239503	37.0	35.0	37.0	35.0	37.0
8	35.804664415792864	37.0	35.0	37.0	35.0	37.0
9	37.502198857809894	39.0	37.0	39.0	35.0	39.0
10	37.367911132367865	39.0	37.0	39.0	35.0	39.0
11	37.494913916912225	39.0	37.0	39.0	35.0	39.0
12	37.4528404108858	39.0	37.0	39.0	35.0	39.0
13	37.48705207138485	39.0	37.0	39.0	35.0	39.0
14	38.75201477524363	40.0	38.0	41.0	36.0	41.0
15	38.75184183896119	40.0	38.0	41.0	35.0	41.0
16	38.631597080078954	40.0	38.0	41.0	35.0	41.0
17	38.6729720856521	40.0	38.0	41.0	35.0	41.0
18	38.6865205625293	40.0	38.0	41.0	35.0	41.0
19	38.74129407679722	40.0	38.0	41.0	35.0	41.0
20	38.71607780511432	40.0	38.0	41.0	35.0	41.0
21	38.68533567878166	40.0	38.0	41.0	35.0	41.0
22	38.662991770664874	40.0	38.0	41.0	35.0	41.0
23	38.62167486087411	40.0	38.0	41.0	35.0	41.0
24	38.55840720279616	40.0	38.0	41.0	34.0	41.0
25	38.48121952504671	40.0	38.0	41.0	34.0	41.0
26	38.38807036885417	40.0	38.0	41.0	34.0	41.0
27	38.31099077628125	40.0	38.0	41.0	34.0	41.0
28	38.23421787539671	40.0	38.0	41.0	34.0	41.0
29	38.09828590420681	40.0	38.0	41.0	34.0	41.0
30	38.05086488407189	40.0	38.0	41.0	34.0	41.0
31	37.962310698946304	40.0	37.0	41.0	33.0	41.0
32	37.882431700301424	40.0	37.0	41.0	33.0	41.0
33	37.73932962871391	40.0	37.0	41.0	33.0	41.0
34	37.741060342602985	40.0	37.0	41.0	33.0	41.0
35	37.7057070324269	40.0	37.0	41.0	33.0	41.0
36	37.69626309012818	40.0	37.0	41.0	33.0	41.0
37	37.66344167521215	40.0	37.0	41.0	33.0	41.0
38	37.589235797270035	40.0	37.0	41.0	33.0	41.0
39	37.40355221932644	39.0	37.0	41.0	32.0	41.0
40	37.42146868839989	39.0	37.0	41.0	32.0	41.0
41	37.428083501203126	39.0	37.0	41.0	32.0	41.0
42	37.321110250933245	39.0	36.0	41.0	32.0	41.0
43	36.44618641720608	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	1.0
14	2.0
15	2.0
16	2.0
17	15.0
18	32.0
19	56.0
20	86.0
21	167.0
22	310.0
23	523.0
24	851.0
25	1306.0
26	1995.0
27	2726.0
28	4075.0
29	5655.0
30	7665.0
31	10520.0
32	14308.0
33	19415.0
34	27851.0
35	41465.0
36	66808.0
37	120357.0
38	232193.0
39	181769.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.22171782473178	18.62091421144433	12.83322322155975	26.324144742264146
2	19.88483524441436	20.650483613611705	33.5526111352051	25.912070006768833
3	21.082148787351873	21.713771537660254	29.42010951730511	27.783970157682763
4	16.23777117557491	15.809078344189137	32.99164906904886	34.961501411187086
5	16.315727609142385	34.47349683918412	33.27456201859876	15.936213533074739
6	35.66770293329659	33.20849495444885	15.298375885116267	15.825426227138294
7	29.59817984562735	29.15624658011746	20.863546517833377	20.382027056421816
8	27.101006948525786	32.323412465193194	20.161668402784816	20.413912183496205
9	26.62786408829478	14.230629447536131	19.63542869958671	39.50607776458238
10	17.758394502787922	26.33981709286003	32.87761920781672	23.02416919653533
11	36.29365121183749	21.09390305029879	20.8481443801788	21.76430135768492
12	22.095041997846405	24.77541926915506	28.31520880029507	24.814329932703465
13	31.02557970808896	19.29725720353925	23.94370383580781	25.733459252563982
14	23.70956432216408	20.657914469497687	23.944649581102386	31.687871627235843
15	26.241054262811808	26.906588737254395	21.499357568732037	25.352999431201756
16	25.964761530323972	25.53336656952511	23.27033318606728	25.231538714083634
17	24.45373076252741	25.739268830802114	24.864454433316176	24.942545973354303
18	24.30551896421975	24.710027737358427	25.8514072014451	25.133046096976724
19	25.362051564735587	24.86661613684664	25.597136823673893	24.174195474743872
20	25.67022942429782	24.33375621658648	25.430550545357267	24.56546381375843
21	25.587814477198755	24.742318183844777	24.46440417370909	25.205463165247373
22	25.776017790820056	24.45886480841227	24.601807454364412	25.163309946403263
23	25.28558130234531	24.68476282734609	24.678818142637304	25.350837727671294
24	25.6278059925124	24.830948028593934	24.592485107889274	24.948760871004396
25	25.743862450804357	24.185003992396208	24.648284080269455	25.42284947652998
26	25.315845151771853	24.85486187389973	24.55033188904516	25.278961085283257
27	25.351648366495215	24.478185033715818	24.60694150024927	25.563225099539693
28	24.97726833631243	24.545468056101612	25.07022158812252	25.40704201946344
29	24.77379799150721	24.856212938606266	25.28233874704961	25.087650322836915
30	24.869858692142344	25.415553727114652	24.999966223382337	24.71462135736067
31	25.41568883358531	24.66152451439357	24.875533163909818	25.047253488111306
32	24.540469116687404	24.529120173152453	25.27747491410606	25.652935796054084
33	24.77312245915394	24.43346479192928	25.25383128174158	25.53958146717521
34	25.26382916056999	24.068677321162944	25.13237056462345	25.53512295364362
35	24.891205514505707	24.05786880351061	25.689414543130713	25.361511138852972
36	24.675845800282914	23.98301981876818	25.771018851405852	25.57011552954306
37	24.978349188077665	22.803675436427675	26.241864901635736	25.976110473858927
38	24.24485615889602	23.087399024801496	27.283670896850264	25.384073919452227
39	24.05327518350836	22.77354669347179	27.70912117294034	25.46405695007951
40	23.45988756439512	22.48522948509573	28.399380131512636	25.65550281899651
41	22.791650960539453	22.712343462265437	28.8726580982143	25.62334747898081
42	23.168868226605976	22.473880541560774	28.53935043511039	25.81790079672286
43	22.254737846159667	22.638845542229554	29.005873078279336	26.100543533331443
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	43.0
1	43.5
2	44.0
3	101.0
4	158.0
5	158.0
6	193.5
7	229.0
8	235.5
9	242.0
10	362.0
11	482.0
12	482.0
13	780.5
14	1079.0
15	1626.0
16	2173.0
17	2294.5
18	2416.0
19	2416.0
20	3245.0
21	4074.0
22	4613.5
23	5153.0
24	6285.5
25	7418.0
26	7418.0
27	8623.5
28	9829.0
29	12287.0
30	14745.0
31	17100.5
32	19456.0
33	19456.0
34	21450.5
35	23445.0
36	26464.0
37	29483.0
38	32500.5
39	35518.0
40	35518.0
41	38617.5
42	41717.0
43	42258.5
44	42800.0
45	45037.5
46	47275.0
47	47275.0
48	48832.0
49	50389.0
50	53125.0
51	55861.0
52	59402.0
53	62943.0
54	62943.0
55	58906.5
56	54870.0
57	52982.0
58	51094.0
59	46935.5
60	42777.0
61	42777.0
62	39876.0
63	36975.0
64	33211.5
65	29448.0
66	25692.0
67	21936.0
68	21936.0
69	18837.5
70	15739.0
71	13789.0
72	11839.0
73	9773.5
74	7708.0
75	7708.0
76	6105.5
77	4503.0
78	3741.0
79	2979.0
80	2264.0
81	1549.0
82	1549.0
83	1214.5
84	880.0
85	680.0
86	480.0
87	373.0
88	266.0
89	266.0
90	191.5
91	117.0
92	65.5
93	14.0
94	11.0
95	8.0
96	8.0
97	5.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	740157.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.75214220058031
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.25733175542778	62.95642239249551
2	5.604021437928554	7.81785000466986
3	1.515512007697997	3.1713062720291294
4	0.6825358123190249	1.9043334015146087
5	0.38789453892730336	1.3528237519042898
6	0.2705128715984881	1.1321311372095035
7	0.1898347128560651	0.9268964520019808
8	0.14233436086816914	0.7942501263444198
9	0.10671432745862888	0.6699197649360216
>10	0.704797449841402	9.728580402639931
>50	0.09726926331221192	4.706285836610085
>100	0.040657856173898894	4.556281663698832
>500	5.836055904782776E-4	0.2829187939458564
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	837	0.1130841159375646	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	1.3510647065419904E-4	0.0	0.0	0.0	0.0
11	1.3510647065419904E-4	0.0	0.0	0.0	0.0
12	1.3510647065419904E-4	0.0	0.0	0.0	0.0
13	1.3510647065419904E-4	0.0	0.0	0.0	0.0
14	1.3510647065419904E-4	0.0	0.0	0.0	0.0
15	1.3510647065419904E-4	0.0	0.0	0.0	0.0
16	1.3510647065419904E-4	0.0	0.0	1.3510647065419904E-4	0.0
17	1.3510647065419904E-4	0.0	0.0	4.0531941196259713E-4	0.0
18	1.3510647065419904E-4	0.0	0.0	5.404258826167961E-4	0.0
19	1.3510647065419904E-4	0.0	0.0	5.404258826167961E-4	0.0
20	1.3510647065419904E-4	0.0	0.0	5.404258826167961E-4	0.0
21	1.3510647065419904E-4	0.0	0.0	6.755323532709952E-4	0.0
22	1.3510647065419904E-4	0.0	0.0	0.0010808517652335923	0.0
23	1.3510647065419904E-4	0.0	0.0	0.0018914905891587867	0.0
24	1.3510647065419904E-4	0.0	0.0	0.003918087648971772	0.0
25	1.3510647065419904E-4	0.0	0.0	0.006214897650093156	0.0
26	1.3510647065419904E-4	0.0	0.0	0.00986277235775653	0.0
27	1.3510647065419904E-4	0.0	0.0	0.03472236295812915	0.0
28	2.7021294130839807E-4	0.0	0.0	0.09754687181233171	0.0
29	2.7021294130839807E-4	0.0	0.0	0.1705043659655992	0.0
30	2.7021294130839807E-4	0.0	0.0	0.27413102895736985	0.0
31	2.7021294130839807E-4	0.0	0.0	0.5733918614564207	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGAAC	25	0.005495095	29.599998	29
GGTATCA	460	0.0	23.728262	1
AGCGTTC	50	0.007032574	18.499998	27
CTTATAC	535	0.0	17.981308	37
ATTGTAC	65	0.0015792155	17.076923	3
CGATAAC	65	0.0015792155	17.076923	10
GTATCAA	670	0.0	16.291044	2
GGACCAT	80	3.38053E-4	16.1875	6
ACCGTCC	70	0.002591324	15.857143	8
CGCAAGA	110	1.4504003E-5	15.136364	2
CCGATCG	75	0.0041025705	14.8	18
GGACTAG	75	0.0041025705	14.8	1
AGCGATA	75	0.0041025705	14.8	21
GCGATAA	75	0.0041025705	14.8	22
ATACCTT	75	0.0041025705	14.8	6
CGATAAA	75	0.0041025705	14.8	23
CTACACT	200	6.184564E-11	14.799999	4
TCTTATA	870	0.0	14.672413	37
CTCTAGA	115	2.2076325E-5	14.478261	1
TTTACAC	90	8.269105E-4	14.388889	3
>>END_MODULE