FastQCFastQC Report
Fri 10 Feb 2017
ERR1632260.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632260.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145401
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT39242.698743474941713No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37782.5983315107874083No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22891.5742670270493326No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13780.9477238808536393No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6320.43466000921589265No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT5840.4016478566172172RNA PCR Primer, Index 47 (95% over 22bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3940.27097475258079384No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2940.20219943466688672No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC2940.20219943466688672No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT2730.18775661790496626No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2300.1581832312019862No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC2100.14442816761920482No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2080.1430526612609267No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA1990.13686288264867502No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG1810.12448332542417179No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA1700.11691804045364199No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1650.11347927455794664No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC1640.11279152137880757RNA PCR Primer, Index 47 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1600.11004050866225128No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.10728949594569501No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT1520.10453848322913872No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1490.1024752236917215No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG250.005480762629.5999985
GCCGTCT952.746674E-1023.36842236
ACCGCCC555.113459E-420.1818188
ATGCCGT1205.0640665E-918.534
TGCCGTC1205.0640665E-918.535
ACCGGGC500.007006088318.4999988
ACACCGC500.007006088318.4999986
GGCGTCA500.007006088318.49999816
GGGGTTT500.007006088318.49999837
CTCTTAT2200.017.6590937
GGTATCA17850.017.2044811
TATGCCG1301.36406015E-817.07692333
CCGTCTT1301.36406015E-817.07692337
GAGAGGG1201.0228359E-716.9583347
GGGCAGT1757.2759576E-1216.9142867
ATACACA700.002577919515.8571434
GTGATTC1659.458745E-1015.6969716
CGCCTTA1302.5436202E-715.65384625
TGGGCAG1902.7284841E-1115.5789486
CGGTGAT1801.9463187E-1015.41666614
Produced by FastQC (version 0.11.5)