##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632260.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	145401
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.967737498366585	33.0	31.0	34.0	30.0	34.0
2	32.059222426255666	33.0	31.0	34.0	30.0	34.0
3	31.954092475292466	33.0	31.0	34.0	30.0	34.0
4	35.7575463717581	37.0	35.0	37.0	33.0	37.0
5	35.502073575835105	37.0	35.0	37.0	33.0	37.0
6	35.57346923336153	37.0	35.0	37.0	33.0	37.0
7	35.54349007228286	37.0	35.0	37.0	33.0	37.0
8	35.568448635153814	37.0	35.0	37.0	33.0	37.0
9	37.15731666219627	39.0	37.0	39.0	34.0	39.0
10	36.97363154311181	39.0	37.0	39.0	33.0	39.0
11	37.17571405973824	39.0	37.0	39.0	34.0	39.0
12	37.098444990061964	39.0	37.0	39.0	33.0	39.0
13	37.19549384117028	39.0	37.0	39.0	34.0	39.0
14	38.164916334825755	40.0	38.0	41.0	34.0	41.0
15	38.16684204372735	40.0	38.0	41.0	34.0	41.0
16	37.9428820984725	39.0	37.0	41.0	33.0	41.0
17	38.13233746672994	40.0	38.0	41.0	34.0	41.0
18	38.259606192529624	40.0	38.0	41.0	34.0	41.0
19	38.35688200218706	40.0	38.0	41.0	34.0	41.0
20	38.38065762958989	40.0	38.0	41.0	34.0	41.0
21	38.36492183685119	40.0	38.0	41.0	34.0	41.0
22	38.27338188870778	40.0	38.0	41.0	34.0	41.0
23	38.2042695717361	40.0	38.0	41.0	34.0	41.0
24	38.09515752986568	40.0	37.0	41.0	34.0	41.0
25	37.991843247295414	40.0	37.0	41.0	33.0	41.0
26	37.77428628413835	39.0	37.0	41.0	33.0	41.0
27	37.60759554611041	39.0	36.0	41.0	33.0	41.0
28	37.462424605057734	39.0	36.0	40.0	33.0	41.0
29	37.20388442995578	39.0	35.0	40.0	33.0	41.0
30	36.99164379887346	39.0	35.0	40.0	32.0	41.0
31	36.67141904113452	38.0	35.0	40.0	32.0	41.0
32	36.38187495271697	38.0	35.0	40.0	31.0	41.0
33	36.06281249785077	38.0	35.0	40.0	30.0	41.0
34	35.92824671082042	38.0	35.0	40.0	30.0	41.0
35	35.70791810235143	38.0	35.0	40.0	30.0	41.0
36	35.52338704685663	38.0	35.0	40.0	29.0	41.0
37	35.2831961265741	38.0	35.0	40.0	28.0	41.0
38	35.026622925564475	37.0	34.0	40.0	26.0	41.0
39	34.573421090638995	37.0	34.0	40.0	24.0	41.0
40	34.33091244214276	37.0	33.0	40.0	23.0	41.0
41	34.028693062633685	37.0	33.0	40.0	21.0	41.0
42	33.6131182041389	36.0	33.0	40.0	18.0	41.0
43	32.679135631804456	35.0	32.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	0.0
13	2.0
14	1.0
15	0.0
16	2.0
17	4.0
18	8.0
19	16.0
20	32.0
21	69.0
22	100.0
23	227.0
24	339.0
25	496.0
26	846.0
27	1256.0
28	1765.0
29	2329.0
30	3100.0
31	3909.0
32	4903.0
33	6242.0
34	8517.0
35	12276.0
36	19152.0
37	31206.0
38	32307.0
39	16292.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.576715428367066	19.34993569507775	15.737855998239352	19.33549287831583
2	19.457912944202587	24.03559810455224	32.69509838309227	23.811390568152902
3	22.083066828976417	25.272866073823426	32.215046664053204	20.429020433146952
4	14.206229668296642	18.616790806115503	30.32372542142076	36.8532541041671
5	12.479281435478436	39.73631543111808	31.44888962249228	16.335513510911206
6	28.87119070707904	32.79894911314227	17.999188451248614	20.330671728530064
7	25.093362494068128	34.96399612107207	20.278402486915496	19.664238897944305
8	32.28588524150453	30.475031120831357	19.092716006079737	18.146367631584376
9	27.966107523332028	11.600332872538702	21.270142571234036	39.16341703289524
10	21.110583833673772	28.9461558036052	27.198574975412825	22.744685387308202
11	31.42069174214758	23.93793715311449	18.294234565099277	26.347136539638655
12	20.088582609473114	33.43512080384591	26.153877896300575	20.322418690380395
13	33.3099497252426	19.384323354034706	26.81687196099064	20.488854959732052
14	23.356097963562835	23.704101072207205	28.77903178107441	24.16076918315555
15	30.157289152069104	25.627746714259185	24.578923116072104	19.636041017599602
16	22.29558256133039	24.49983150047111	29.92276531798268	23.281820620215818
17	19.457225191023444	30.669665270527712	28.22745373140487	21.64565580704397
18	18.796294385870798	23.614693158919128	32.585745627609164	25.00326682760091
19	20.632595374172116	27.659369605436	34.33332645580155	17.374708564590343
20	22.00741397927112	22.936568524288003	35.726026643558164	19.329990852882716
21	25.129125659383362	22.950323587870784	32.638702622402874	19.281848130342983
22	23.985392122475087	24.163520195872103	32.09950412995784	19.751583551694967
23	22.078940309901583	25.589232536227396	32.210920144978374	20.12090700889265
24	19.797663014697285	27.14493022743998	33.03553620676611	20.021870551096622
25	19.869877098506887	27.03145095288203	32.28450973514625	20.814162213464833
26	20.938645538889002	25.9571804870668	32.43994195363168	20.664232020412516
27	19.54250658523669	27.213017792174742	32.29826479872903	20.946210823859534
28	19.34856018871947	27.180693392755206	33.746672993995915	19.724073424529404
29	19.125728158678413	27.59472080659693	33.53209400210452	19.747457032620133
30	18.75090267604762	28.333367720992293	34.308567341352536	18.607162261607556
31	19.13673220954464	28.66073823426249	32.57061505766811	19.631914498524768
32	18.234400038514178	29.423456509927714	33.48739004546049	18.85475340609762
33	18.592031691666495	28.814107193210504	33.51352466626777	19.080336448855235
34	18.51500333560292	29.53968679720222	32.00046767216181	19.944842195033047
35	17.98680889402411	29.77008411221381	32.8415898102489	19.40151718351318
36	18.316242666831727	30.188238045130362	31.263196264124733	20.232323023913178
37	17.838941960509214	30.219874691370762	31.589191271036647	20.351992077083374
38	18.208265417706894	29.30103644404096	31.89249042303698	20.598207715215164
39	18.446228017689013	29.301724197220103	32.164152928796916	20.08789485629397
40	17.79492575704431	29.057571818625732	32.66071072413532	20.486791700194633
41	17.125054160562858	29.624280438236326	32.358099325314136	20.892566075886688
42	17.340320905633387	29.423456509927714	32.53760290506943	20.698619679369468
43	16.629184118403586	30.107083169991956	32.10844492128665	21.15528779031781
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	28.5
2	53.0
3	193.5
4	334.0
5	334.0
6	519.0
7	704.0
8	760.5
9	817.0
10	1090.0
11	1363.0
12	1363.0
13	2587.5
14	3812.0
15	5619.0
16	7426.0
17	6425.0
18	5424.0
19	5424.0
20	6189.5
21	6955.0
22	4873.5
23	2792.0
24	2560.0
25	2328.0
26	2328.0
27	2539.0
28	2750.0
29	2990.5
30	3231.0
31	3352.0
32	3473.0
33	3473.0
34	3422.0
35	3371.0
36	3445.5
37	3520.0
38	4198.5
39	4877.0
40	4877.0
41	5539.5
42	6202.0
43	6932.5
44	7663.0
45	8498.5
46	9334.0
47	9334.0
48	10883.5
49	12433.0
50	11716.0
51	10999.0
52	10231.0
53	9463.0
54	9463.0
55	8412.0
56	7361.0
57	7442.0
58	7523.0
59	6707.5
60	5892.0
61	5892.0
62	5031.0
63	4170.0
64	3730.5
65	3291.0
66	2872.5
67	2454.0
68	2454.0
69	2203.0
70	1952.0
71	1651.0
72	1350.0
73	1150.0
74	950.0
75	950.0
76	749.0
77	548.0
78	424.0
79	300.0
80	237.5
81	175.0
82	175.0
83	120.5
84	66.0
85	48.0
86	30.0
87	19.5
88	9.0
89	9.0
90	5.5
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	145401.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.89299936038954
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.2971001580004	48.22318966169421
2	9.408745856944297	10.894010357562877
3	2.899841999596089	5.036416530835414
4	1.248559582783896	2.8913143651006523
5	0.7104078311177637	2.056382005625821
6	0.4775651306176272	1.6588606680834381
7	0.3266925644772325	1.3239248698427108
8	0.2387825653088136	1.1059071120556254
9	0.21621107903584116	1.1265397074297976
>10	1.066799719638381	11.186305458696982
>50	0.07246635066585884	2.7812738564384016
>100	0.029699324043384774	3.060501647168864
>500	0.002375945923470782	0.8363078658331098
>1k	0.004751891846941564	7.819065893632093
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3924	2.698743474941713	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3778	2.5983315107874083	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2289	1.5742670270493326	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1378	0.9477238808536393	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	632	0.43466000921589265	No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT	584	0.4016478566172172	RNA PCR Primer, Index 47 (95% over 22bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	394	0.27097475258079384	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	294	0.20219943466688672	No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC	294	0.20219943466688672	No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT	273	0.18775661790496626	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	230	0.1581832312019862	No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC	210	0.14442816761920482	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	208	0.1430526612609267	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	199	0.13686288264867502	No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG	181	0.12448332542417179	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	170	0.11691804045364199	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	165	0.11347927455794664	No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC	164	0.11279152137880757	RNA PCR Primer, Index 47 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	160	0.11004050866225128	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	156	0.10728949594569501	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	152	0.10453848322913872	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	149	0.1024752236917215	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	6.877531791390706E-4	0.0
13	0.0	0.0	0.0	6.877531791390706E-4	0.0
14	0.0	0.0	0.0	0.0013755063582781412	0.0
15	0.0	0.0	0.0	0.0013755063582781412	0.0
16	0.0	0.0	0.0	0.002063259537417212	0.0
17	0.0	6.877531791390706E-4	0.0	0.004126519074834424	0.0
18	0.0	6.877531791390706E-4	0.0	0.004814272253973494	0.0
19	0.0	6.877531791390706E-4	0.0	0.006189778612251635	0.0
20	0.0	6.877531791390706E-4	0.0	0.006877531791390706	0.0
21	0.0	6.877531791390706E-4	0.0	0.008940791328807917	0.0
22	0.0	6.877531791390706E-4	0.0	0.0116918040453642	0.0
23	0.0	6.877531791390706E-4	0.0	0.015130569941059553	0.0
24	0.0	6.877531791390706E-4	0.0	0.02613462080728468	0.0
25	0.0	6.877531791390706E-4	0.0	0.028197880344701894	0.0
26	0.0	6.877531791390706E-4	0.0	0.035075412136092596	0.0
27	0.0	6.877531791390706E-4	0.0	0.04814272253973494	0.0
28	0.0	6.877531791390706E-4	0.0	0.08596914739238382	0.0
29	0.0	6.877531791390706E-4	0.0	0.14305266126092667	0.0
30	0.0	6.877531791390706E-4	0.0	0.2757890248347673	0.0
31	0.0	6.877531791390706E-4	0.0	0.5543290623860909	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACACCG	25	0.0054807626	29.599998	5
GCCGTCT	95	2.746674E-10	23.368422	36
ACCGCCC	55	5.113459E-4	20.181818	8
ATGCCGT	120	5.0640665E-9	18.5	34
TGCCGTC	120	5.0640665E-9	18.5	35
ACCGGGC	50	0.0070060883	18.499998	8
ACACCGC	50	0.0070060883	18.499998	6
GGCGTCA	50	0.0070060883	18.499998	16
GGGGTTT	50	0.0070060883	18.499998	37
CTCTTAT	220	0.0	17.65909	37
GGTATCA	1785	0.0	17.204481	1
TATGCCG	130	1.36406015E-8	17.076923	33
CCGTCTT	130	1.36406015E-8	17.076923	37
GAGAGGG	120	1.0228359E-7	16.958334	7
GGGCAGT	175	7.2759576E-12	16.914286	7
ATACACA	70	0.0025779195	15.857143	4
GTGATTC	165	9.458745E-10	15.69697	16
CGCCTTA	130	2.5436202E-7	15.653846	25
TGGGCAG	190	2.7284841E-11	15.578948	6
CGGTGAT	180	1.9463187E-10	15.416666	14
>>END_MODULE