FastQCFastQC Report
Fri 10 Feb 2017
ERR1632259.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632259.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124810
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCCG250.00547782229.59999810
AGTAAGA250.00547782229.5999982
GGTATCA451.3140019E-424.6666661
GTCTTGG451.3140019E-424.6666661
GAGTAGG450.003807014320.5555551
AGGAGAG701.2081842E-418.55
CTCTTAT2550.017.41176437
GCTGAGC750.00407720914.88
TCTTATA1254.777134E-513.3237
TCTCTTA3700.012.036
TGTCTCT7100.09.90140837
GCCTGTC1500.0032662049.86666735
TCTGTCT2708.9812784E-79.59259236
CTGTCTC9400.09.2537
GTCTCTT4801.8189894E-129.2535
CCTGTCT4908.268909E-66.41836735
GCTGTCT3600.0024443866.166666536
Produced by FastQC (version 0.11.5)