##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632259.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	124810
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54333787356782	34.0	31.0	34.0	31.0	34.0
2	32.718339876612454	34.0	31.0	34.0	31.0	34.0
3	32.72765804022114	34.0	31.0	34.0	31.0	34.0
4	36.25302459738803	37.0	37.0	37.0	35.0	37.0
5	36.11398926368079	37.0	35.0	37.0	35.0	37.0
6	36.15368159602596	37.0	35.0	37.0	35.0	37.0
7	36.15230350132201	37.0	35.0	37.0	35.0	37.0
8	36.10942232192933	37.0	35.0	37.0	35.0	37.0
9	37.87698902331544	39.0	38.0	39.0	35.0	39.0
10	37.78739684320167	39.0	37.0	39.0	35.0	39.0
11	37.89707555484336	39.0	38.0	39.0	35.0	39.0
12	37.85376972998959	39.0	38.0	39.0	35.0	39.0
13	37.882597548273374	39.0	38.0	39.0	35.0	39.0
14	39.23806586010736	40.0	39.0	41.0	36.0	41.0
15	39.248337472958895	40.0	39.0	41.0	36.0	41.0
16	39.148193253745696	40.0	39.0	41.0	36.0	41.0
17	39.180594503645544	40.0	39.0	41.0	36.0	41.0
18	39.187388831023156	40.0	39.0	41.0	36.0	41.0
19	39.23479689127474	40.0	39.0	41.0	36.0	41.0
20	39.20421440589696	40.0	39.0	41.0	36.0	41.0
21	39.17365595705472	40.0	39.0	41.0	36.0	41.0
22	39.13761717811073	40.0	39.0	41.0	36.0	41.0
23	39.10280426247897	40.0	39.0	41.0	36.0	41.0
24	39.061076836791926	40.0	39.0	41.0	36.0	41.0
25	38.98811793926769	40.0	38.0	41.0	36.0	41.0
26	38.911994231231475	40.0	38.0	41.0	35.0	41.0
27	38.83018187645221	40.0	38.0	41.0	35.0	41.0
28	38.76921721015944	40.0	38.0	41.0	35.0	41.0
29	38.63807387228587	40.0	38.0	41.0	35.0	41.0
30	38.5722297892797	40.0	38.0	41.0	35.0	41.0
31	38.51896482653633	40.0	38.0	41.0	34.0	41.0
32	38.4408460860508	40.0	38.0	41.0	34.0	41.0
33	38.318788558609086	40.0	38.0	41.0	34.0	41.0
34	38.31600833266565	40.0	38.0	41.0	34.0	41.0
35	38.28817402451727	40.0	38.0	41.0	34.0	41.0
36	38.27022674465187	40.0	38.0	41.0	34.0	41.0
37	38.23514942712924	40.0	38.0	41.0	34.0	41.0
38	38.163536575594904	40.0	38.0	41.0	34.0	41.0
39	37.99169137088374	40.0	37.0	41.0	33.0	41.0
40	37.993854659081805	40.0	37.0	41.0	33.0	41.0
41	37.997660443874686	40.0	37.0	41.0	33.0	41.0
42	37.902299495232754	40.0	37.0	41.0	33.0	41.0
43	37.00483935582085	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	0.0
18	2.0
19	4.0
20	14.0
21	18.0
22	39.0
23	58.0
24	96.0
25	184.0
26	239.0
27	333.0
28	490.0
29	664.0
30	932.0
31	1291.0
32	1764.0
33	2354.0
34	3578.0
35	5382.0
36	8614.0
37	15543.0
38	36433.0
39	46776.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.45725502764202	17.384023716048393	11.653713644740005	27.50500761156959
2	20.999118660363752	19.362230590497557	31.318003365114976	28.32064738402372
3	21.726624469193172	20.3421200224341	27.924044547712523	30.007210960660203
4	16.52271452607964	14.715167053921963	31.430173864273698	37.3319445557247
5	17.747776620463103	33.241727425687046	32.20895761557567	16.801538338274177
6	38.599471196218246	31.517506610047274	14.144699943914752	15.738322249819724
7	31.86363272173704	27.479368640333306	19.739604198381542	20.91739443954811
8	29.347007451326018	30.72990946238282	18.48008973639933	21.442993349891836
9	27.43450044066982	13.056646102075153	17.839115455492347	41.66973800176268
10	18.46566781507892	24.466789520070506	31.695376972998957	25.372165691851617
11	39.53849851774697	19.27249419117058	19.133883502924444	22.055123788158003
12	24.06297572309911	22.801057607563497	26.880057687685284	26.25590898165211
13	33.299415110968674	17.488983254546913	21.528723659963145	27.682877974521276
14	24.814518067462544	18.581043185642176	21.933338674785674	34.67110007210961
15	28.085890553641534	24.672702507811874	19.7932857944075	27.448121144139094
16	27.704510856501884	23.29941511096867	21.801938947199744	27.1941350853297
17	26.596426568383947	23.24893838634725	22.82990145020431	27.324733595064497
18	26.631680153833827	22.68568223700024	23.717650829260474	26.96498677990546
19	27.516224661485456	22.820286835990704	23.087092380418238	26.5763961221056
20	27.744571749058565	22.601554362631198	22.840317282269048	26.813556606041182
21	27.799855780786796	22.543065459498436	22.562294687925647	27.094784071789118
22	28.295008412787436	22.515022834708756	22.163288198061053	27.026680554442756
23	27.693293806586013	22.592740966268728	22.32673663969233	27.387228587452928
24	27.432096787116418	22.809871003925966	22.19453569425527	27.563496514702347
25	27.705312074353017	22.23619902251422	22.584728787757392	27.473760115375374
26	27.49298934380258	22.439708356702187	22.751382100793204	27.315920198702027
27	27.296690970274817	22.351574393077478	22.66004326576396	27.691691370883742
28	27.21176187805464	22.52864353817803	22.753785754346605	27.50580882942072
29	26.950564858585047	22.858745292845125	23.13115936223059	27.059530486339234
30	27.072349971957376	23.067863151991027	22.636006730229948	27.223780145821646
31	27.43930774777662	22.684079801297973	22.593542184119862	27.28307026680554
32	26.794327377613975	22.953288999278904	22.72814678311033	27.524236839996796
33	26.602836311193013	22.658440830061693	23.26416152551879	27.474561333226504
34	27.160483935582086	22.021472638410383	23.171220254787276	27.64682317122025
35	26.633282589536094	22.31311593622306	24.044547712523034	27.009053761717812
36	26.45941831584008	22.3331463825014	23.752103196859224	27.455332104799297
37	26.201426167775015	21.55195897764602	24.64145501161766	27.605159842961303
38	25.595705472317924	21.57359185962663	25.402611970194695	27.428090697860746
39	25.490745933819404	21.160964666292763	26.05560451886868	27.29268488101915
40	25.0701065619742	20.95184680714686	26.73744091018348	27.240605720695456
41	24.42192132040702	20.9574553321048	27.072349971957376	27.54827337553081
42	24.02692091979809	21.019149106642097	27.50420639371845	27.449723579841358
43	23.62390834067783	20.623347488182038	27.795048473679994	27.95769569746014
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.5
2	3.0
3	6.0
4	9.0
5	9.0
6	8.0
7	7.0
8	7.0
9	7.0
10	8.5
11	10.0
12	10.0
13	21.0
14	32.0
15	53.5
16	75.0
17	79.0
18	83.0
19	83.0
20	108.5
21	134.0
22	163.5
23	193.0
24	289.0
25	385.0
26	385.0
27	505.5
28	626.0
29	814.0
30	1002.0
31	1276.0
32	1550.0
33	1550.0
34	1939.0
35	2328.0
36	2823.0
37	3318.0
38	3827.5
39	4337.0
40	4337.0
41	4858.0
42	5379.0
43	6060.5
44	6742.0
45	7273.5
46	7805.0
47	7805.0
48	8163.5
49	8522.0
50	9122.5
51	9723.0
52	10050.5
53	10378.0
54	10378.0
55	10480.5
56	10583.0
57	10426.0
58	10269.0
59	10056.5
60	9844.0
61	9844.0
62	9171.5
63	8499.0
64	7679.0
65	6859.0
66	6028.0
67	5197.0
68	5197.0
69	4508.5
70	3820.0
71	3227.0
72	2634.0
73	2156.5
74	1679.0
75	1679.0
76	1409.5
77	1140.0
78	934.0
79	728.0
80	586.0
81	444.0
82	444.0
83	338.5
84	233.0
85	186.5
86	140.0
87	97.5
88	55.0
89	55.0
90	40.5
91	26.0
92	16.5
93	7.0
94	5.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	124810.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.26465093716281
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.48294714464983	80.70492100217946
2	4.844081461732197	8.454341557384831
3	1.2566053296308177	3.2897167636803526
4	0.5547829096860105	1.936517478386129
5	0.2828648845694828	1.2342052707168383
6	0.1929062070584196	1.0100335693539024
7	0.10095083139550504	0.6166607344481587
8	0.07796202169507561	0.5442662887661031
9	0.058971272333562655	0.4631496745957841
>10	0.14792793724910622	1.7461876604884365
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0016024357022674466	0.0
18	0.0	0.0	0.0	0.00240365355340117	0.0
19	0.0	0.0	0.0	0.00240365355340117	0.0
20	0.0	0.0	0.0	0.00240365355340117	0.0
21	0.0	0.0	0.0	0.00240365355340117	0.0
22	0.0	0.0	0.0	0.00240365355340117	0.0
23	0.0	0.0	0.0	0.0040060892556686165	0.0
24	0.0	0.0	0.0	0.005608524957936063	0.0
25	0.0	0.0	0.0	0.00721096066020351	0.0
26	0.0	0.0	0.0	0.010415832064738402	0.0
27	0.0	0.0	0.0	0.024036535534011697	0.0
28	0.0	0.0	0.0	0.08092300296450605	0.0
29	0.0	0.0	0.0	0.1378094703950004	0.0
30	0.0	0.0	0.0	0.22994952327537857	0.0
31	0.0	0.0	0.0	0.48313436423363515	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGACCCG	25	0.005477822	29.599998	10
AGTAAGA	25	0.005477822	29.599998	2
GGTATCA	45	1.3140019E-4	24.666666	1
GTCTTGG	45	1.3140019E-4	24.666666	1
GAGTAGG	45	0.0038070143	20.555555	1
AGGAGAG	70	1.2081842E-4	18.5	5
CTCTTAT	255	0.0	17.411764	37
GCTGAGC	75	0.004077209	14.8	8
TCTTATA	125	4.777134E-5	13.32	37
TCTCTTA	370	0.0	12.0	36
TGTCTCT	710	0.0	9.901408	37
GCCTGTC	150	0.003266204	9.866667	35
TCTGTCT	270	8.9812784E-7	9.592592	36
CTGTCTC	940	0.0	9.25	37
GTCTCTT	480	1.8189894E-12	9.25	35
CCTGTCT	490	8.268909E-6	6.418367	35
GCTGTCT	360	0.002444386	6.1666665	36
>>END_MODULE