FastQCFastQC Report
Fri 10 Feb 2017
ERR1632258.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632258.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744570
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGAC303.5982725E-430.8333323
GCGCAAC250.00549511529.611
GGTATCA2500.024.4200021
AACGAAC450.003824560420.55555714
TAAGGGG450.003824560420.5555574
CTTATAC6100.019.71311637
AATAACG902.1501437E-618.52
GTATCAA3150.018.2063482
GTATAAA1653.6379788E-1217.9393941
AGCGTTA953.6029342E-617.52631610
TAACGCC953.6029342E-617.5263164
GGCGTTA650.001579227317.07692331
GAATAAC1301.3907993E-817.0769231
GTGTTAG1107.798517E-716.8181821
TCTTATA9750.016.69743537
AACGCCG1005.872198E-616.6500025
TAGAGTG904.4425717E-516.4444455
CGTTTAT904.4425717E-516.44444531
ATTAGAG803.3805636E-416.18753
TTTAGAC700.002591343815.8571423
Produced by FastQC (version 0.11.5)