##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632258.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	744570
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.779084572303475	34.0	31.0	34.0	31.0	34.0
2	32.94103979478088	34.0	31.0	34.0	31.0	34.0
3	32.945054192352636	34.0	33.0	34.0	31.0	34.0
4	36.392482909598826	37.0	37.0	37.0	35.0	37.0
5	36.30212337322213	37.0	37.0	37.0	35.0	37.0
6	36.33135903944559	37.0	37.0	37.0	35.0	37.0
7	36.3210927112293	37.0	37.0	37.0	35.0	37.0
8	36.30089984823455	37.0	37.0	37.0	35.0	37.0
9	38.10469264139033	39.0	39.0	39.0	37.0	39.0
10	38.025692681682045	39.0	38.0	39.0	35.0	39.0
11	38.13092120284191	39.0	38.0	39.0	37.0	39.0
12	38.09266422230281	39.0	38.0	39.0	37.0	39.0
13	38.1291940314544	39.0	39.0	39.0	37.0	39.0
14	39.53655801334999	41.0	39.0	41.0	37.0	41.0
15	39.54812844997784	41.0	39.0	41.0	37.0	41.0
16	39.43935694427656	41.0	39.0	41.0	37.0	41.0
17	39.47204829633211	40.0	39.0	41.0	37.0	41.0
18	39.4874491317136	41.0	39.0	41.0	37.0	41.0
19	39.5226197671139	41.0	39.0	41.0	37.0	41.0
20	39.499841519266155	41.0	39.0	41.0	37.0	41.0
21	39.46960527552816	41.0	39.0	41.0	37.0	41.0
22	39.44912768443531	41.0	39.0	41.0	37.0	41.0
23	39.41550693688975	40.0	39.0	41.0	37.0	41.0
24	39.35291779147696	40.0	39.0	41.0	36.0	41.0
25	39.279495547765826	40.0	39.0	41.0	36.0	41.0
26	39.18983171494957	40.0	39.0	41.0	36.0	41.0
27	39.12235518487181	40.0	39.0	41.0	36.0	41.0
28	39.04550411646991	40.0	39.0	41.0	35.0	41.0
29	38.89874021247163	40.0	39.0	41.0	35.0	41.0
30	38.85020347314558	40.0	38.0	41.0	35.0	41.0
31	38.791568287736546	40.0	38.0	41.0	35.0	41.0
32	38.72998643512363	40.0	38.0	41.0	35.0	41.0
33	38.58251205393717	40.0	38.0	41.0	35.0	41.0
34	38.57822232966679	40.0	38.0	41.0	35.0	41.0
35	38.544110023234886	40.0	38.0	41.0	35.0	41.0
36	38.51461380394053	40.0	38.0	41.0	35.0	41.0
37	38.479224250238396	40.0	38.0	41.0	34.0	41.0
38	38.4085942221685	40.0	38.0	41.0	34.0	41.0
39	38.21789757846811	40.0	38.0	41.0	34.0	41.0
40	38.222026135890516	40.0	38.0	41.0	34.0	41.0
41	38.22810884134467	40.0	37.0	41.0	34.0	41.0
42	38.092857622520384	40.0	37.0	41.0	34.0	41.0
43	37.19418321984501	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	1.0
16	0.0
17	2.0
18	7.0
19	33.0
20	47.0
21	86.0
22	179.0
23	300.0
24	455.0
25	759.0
26	1061.0
27	1579.0
28	2192.0
29	3062.0
30	4309.0
31	5987.0
32	8479.0
33	12098.0
34	18053.0
35	27920.0
36	45359.0
37	82149.0
38	191502.0
39	338950.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.40670454087594	16.341646856574936	10.99627973192581	28.255368870623315
2	21.045972843386114	18.660837798998077	31.233329304162133	29.059860053453672
3	21.589776649609842	19.466672038894934	27.396886793719865	31.54666451777536
4	16.375894811770554	14.02809675383107	31.092308312180183	38.50370012221819
5	18.169950441194246	32.8296869333978	31.544649932175616	17.455712693232336
6	39.08014021515774	31.20042440603301	13.84920155257397	15.870233826235278
7	33.054246074915724	27.276548880561936	18.593147722846744	21.0760573216756
8	29.854949836818566	30.096297191667677	18.196542971110844	21.852210000402916
9	28.20003491948373	12.851847374994962	17.091609922505608	41.8565077830157
10	19.09625689995568	23.888284513208966	30.680124098472945	26.33533448836241
11	39.70681064238419	18.991632754475738	19.07073881569228	22.230817787447787
12	24.3153766603543	22.24559141517923	26.64826678485569	26.790765139610784
13	33.72778919376284	16.8244758719798	21.08022079858173	28.367514135675627
14	24.97817532266946	17.7851645916435	21.891158655331264	35.345501430355775
15	28.33796688021274	24.442698470258	19.196583262822838	28.022751386706425
16	28.57165880978283	22.789798138522908	20.94000564083968	27.698537410854584
17	27.015861503955303	23.124890876613346	22.318653719596547	27.540593899834803
18	27.69047906845562	22.015794351101977	22.746820312395073	27.546906268047326
19	28.040211128570853	22.261170877150573	22.334904710101135	27.363713284177443
20	28.257517761929705	22.052728420430583	22.125253502021298	27.56450031561841
21	28.172770861033886	22.139355601219496	21.90552936594276	27.782344171803857
22	28.314866298669028	22.072739970721354	21.499388909034746	28.113004821574865
23	28.150610419436724	21.95428233745652	21.79956216339632	28.095545079710437
24	28.088426877258016	22.309118014424435	21.665122151040197	27.937332957277356
25	28.17693433794002	21.49227070658232	21.871415716453793	28.459379239023868
26	27.891669017016536	22.11961266234202	22.059578011469707	27.929140309171736
27	27.860644398780504	22.039700766885584	21.843748740884	28.255906093449912
28	27.60774675315954	22.198047195025318	22.216715688249593	27.97749036356555
29	27.4526236619794	22.572894422284005	22.257544623071034	27.716937292665566
30	27.0041769074768	22.756221711860537	22.119075439515427	28.12052594114724
31	27.8481539680621	22.349409726419275	21.7281115274588	28.074324778059818
32	27.151107350551325	22.288166324187113	22.131834481647125	28.428891843614434
33	26.959587412869173	22.182602038760628	22.777442013511155	28.080368534859048
34	27.560336838712278	21.706891225808185	22.700887760720953	28.031884174758588
35	27.096713539358287	22.032448258726514	23.26000241750272	27.61083578441248
36	26.826356151872893	21.960460399962393	23.120055871173967	28.093127576990746
37	26.67324764629249	21.146030594839974	23.87257074553098	28.30815101333656
38	26.13965107377412	21.17033992774353	24.644962864472113	28.04504613401023
39	25.781189142726674	20.955182185691072	25.10657157822636	28.157057093355895
40	25.506668278335148	20.74969445451737	25.723706300280696	28.01993096686678
41	25.041030393381412	20.636743355225164	26.541225136656056	27.781001114737364
42	25.001813127039767	20.67904965281975	26.73999758249728	27.579139637643202
43	24.093235021556065	20.185341875176277	27.14492928804545	28.57649381522221
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6.0
1	7.0
2	8.0
3	17.5
4	27.0
5	27.0
6	31.5
7	36.0
8	42.5
9	49.0
10	75.5
11	102.0
12	102.0
13	197.0
14	292.0
15	391.5
16	491.0
17	552.5
18	614.0
19	614.0
20	812.0
21	1010.0
22	1205.0
23	1400.0
24	1862.5
25	2325.0
26	2325.0
27	3043.0
28	3761.0
29	4797.0
30	5833.0
31	7233.0
32	8633.0
33	8633.0
34	10531.5
35	12430.0
36	14989.5
37	17549.0
38	20092.0
39	22635.0
40	22635.0
41	25216.0
42	27797.0
43	30719.0
44	33641.0
45	36936.0
46	40231.0
47	40231.0
48	43516.5
49	46802.0
50	49917.0
51	53032.0
52	55330.5
53	57629.0
54	57629.0
55	59423.0
56	61217.0
57	63387.5
58	65558.0
59	65009.5
60	64461.0
61	64461.0
62	60568.0
63	56675.0
64	51148.0
65	45621.0
66	40374.0
67	35127.0
68	35127.0
69	31129.0
70	27131.0
71	23552.0
72	19973.0
73	16252.5
74	12532.0
75	12532.0
76	10261.5
77	7991.0
78	6472.5
79	4954.0
80	4121.0
81	3288.0
82	3288.0
83	2547.0
84	1806.0
85	1419.0
86	1032.0
87	780.5
88	529.0
89	529.0
90	388.0
91	247.0
92	155.0
93	63.0
94	44.0
95	25.0
96	25.0
97	16.0
98	7.0
99	3.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	744570.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.16477590902886
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.95279786545775	65.22999544263787
2	7.280690898744717	10.799416179366158
3	2.057797223294737	4.57848209795628
4	0.901853540776494	2.6754306301781163
5	0.47730998543512304	1.7699794054468865
6	0.32082533584516126	1.4276363483337187
7	0.20893049428605706	1.0846698302501643
8	0.14143338967150731	0.8391500520833359
9	0.1002124505869435	0.6689010546967763
>10	0.4945535020285234	6.576674615913195
>50	0.0450624212709605	2.3278197036148143
>100	0.01853289260210496	2.0218446395227065
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	2.686114132989511E-4	0.0	0.0	0.0
6	0.0	2.686114132989511E-4	0.0	0.0	0.0
7	0.0	2.686114132989511E-4	0.0	0.0	0.0
8	0.0	2.686114132989511E-4	0.0	0.0	0.0
9	0.0	2.686114132989511E-4	0.0	0.0	0.0
10	0.0	2.686114132989511E-4	0.0	0.0	0.0
11	0.0	4.029171199484266E-4	0.0	0.0	0.0
12	0.0	4.029171199484266E-4	0.0	0.0	0.0
13	0.0	4.029171199484266E-4	0.0	0.0	0.0
14	0.0	4.029171199484266E-4	0.0	0.0	0.0
15	0.0	4.029171199484266E-4	0.0	0.0	0.0
16	0.0	4.029171199484266E-4	0.0	0.0	0.0
17	0.0	4.029171199484266E-4	0.0	2.686114132989511E-4	0.0
18	0.0	4.029171199484266E-4	0.0	4.029171199484266E-4	0.0
19	0.0	4.029171199484266E-4	0.0	5.372228265979022E-4	0.0
20	0.0	4.029171199484266E-4	0.0	6.715285332473777E-4	0.0
21	0.0	4.029171199484266E-4	0.0	6.715285332473777E-4	0.0
22	0.0	4.029171199484266E-4	0.0	0.0012087513598452798	0.0
23	0.0	4.029171199484266E-4	0.0	0.001477362773144231	0.0
24	0.0	4.029171199484266E-4	0.0	0.0030890312529379374	0.0
25	0.0	4.029171199484266E-4	0.0	0.004432088319432693	0.0
26	0.0	4.029171199484266E-4	0.0	0.006983896745772728	0.0
27	0.0	4.029171199484266E-4	0.0	0.023637804370307696	0.0
28	0.0	4.029171199484266E-4	0.0	0.08326953812267483	0.0
29	0.0	4.029171199484266E-4	0.0	0.15888365096632956	0.0
30	0.0	4.029171199484266E-4	0.0	0.25853848530024043	0.0
31	0.0	4.029171199484266E-4	0.0	0.5384315779577474	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAAGAC	30	3.5982725E-4	30.833332	3
GCGCAAC	25	0.005495115	29.6	11
GGTATCA	250	0.0	24.420002	1
AACGAAC	45	0.0038245604	20.555557	14
TAAGGGG	45	0.0038245604	20.555557	4
CTTATAC	610	0.0	19.713116	37
AATAACG	90	2.1501437E-6	18.5	2
GTATCAA	315	0.0	18.206348	2
GTATAAA	165	3.6379788E-12	17.939394	1
AGCGTTA	95	3.6029342E-6	17.526316	10
TAACGCC	95	3.6029342E-6	17.526316	4
GGCGTTA	65	0.0015792273	17.076923	31
GAATAAC	130	1.3907993E-8	17.076923	1
GTGTTAG	110	7.798517E-7	16.818182	1
TCTTATA	975	0.0	16.697435	37
AACGCCG	100	5.872198E-6	16.650002	5
TAGAGTG	90	4.4425717E-5	16.444445	5
CGTTTAT	90	4.4425717E-5	16.444445	31
ATTAGAG	80	3.3805636E-4	16.1875	3
TTTAGAC	70	0.0025913438	15.857142	3
>>END_MODULE