FastQCFastQC Report
Fri 10 Feb 2017
ERR1632257.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632257.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52807
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5741.0869771053080084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5040.954418921733861No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT3930.7442195163519988RNA PCR Primer, Index 46 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3270.6192360861249455No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT2450.46395364250951576No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC1950.3692692256708391No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC1710.32382070558827425No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1710.32382070558827425No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG1200.22724260041282404No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC920.1742193269831651RNA PCR Primer, Index 46 (95% over 22bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690.13066449523737383No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.1060465468593179No Hit
GGGTACGCAGGCGGCTAGGAAAAAAAAAAAAAAAAAAAAAAAA550.10415285852254436No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATT550.10415285852254436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1650.031.393941
GATGCTA250.00544956629.611
CCTCACG250.00544956629.614
CTCACGG250.00544956629.615
CCGTCTT556.0737875E-726.9090937
GCCGTCT556.0737875E-726.9090936
TCGCCTA358.7573554E-426.4285737
TAGTACG400.001907022923.1254
TCTGTCT909.0563844E-820.55555537
GTATCAA2550.020.3137252
TACACAT555.054349E-420.1818185
ACTACTC555.054349E-420.1818183
TGCCGTC801.569624E-518.535
ATGCCGT801.569624E-518.534
ATACACA609.078722E-418.54
GGAGTGG500.00694852418.56
TATGCCG852.6443655E-517.41176433
CATGGGA855.244812E-415.23529418
CGTATGC1102.3875383E-413.45454531
TATCAAC3850.013.4545453
Produced by FastQC (version 0.11.5)