FastQCFastQC Report
Fri 10 Feb 2017
ERR1632256.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632256.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151821
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2740.1804756917685959No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2570.169278294834048No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1810.11921934383253964No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGGC250.005481513729.63
TTACACT250.005481513729.64
TAGTTAT358.833833E-426.428577
GGTATCA1303.092282E-1119.9230771
GGCGCAA500.00700747618.534
GTATCAA1451.5097612E-1017.8620682
GGAGTGG904.405203E-516.4444456
TCTCTTA5000.014.05999937
CTCTTAT2950.013.7966137
CTTATAC1356.47705E-613.70370437
TCTTATA1906.9376256E-913.63157937
TATCAAC2457.2759576E-1213.5918361
CAACGCA2457.2759576E-1213.5918364
TCAACGC2457.2759576E-1213.5918363
ATCAACG2501.0913936E-1113.322
CAGGAGT1000.001819783912.9500014
GTCTCTT7300.011.91095837
AACGCAG2808.0035534E-1111.8928575
TTATTCC1257.1554456E-411.844
TTACAGG1200.007114176610.7916673