##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632256.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	151821
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.65665487646636	34.0	31.0	34.0	31.0	34.0
2	32.83734134276549	34.0	31.0	34.0	31.0	34.0
3	32.9114681104722	34.0	31.0	34.0	31.0	34.0
4	36.33689674024015	37.0	37.0	37.0	35.0	37.0
5	36.18715460970485	37.0	35.0	37.0	35.0	37.0
6	36.21906719096831	37.0	37.0	37.0	35.0	37.0
7	36.20365430342311	37.0	36.0	37.0	35.0	37.0
8	36.19377424730439	37.0	36.0	37.0	35.0	37.0
9	37.942959142674596	39.0	38.0	39.0	35.0	39.0
10	37.91897036641835	39.0	38.0	39.0	35.0	39.0
11	37.99013970399352	39.0	38.0	39.0	35.0	39.0
12	37.95465054241508	39.0	38.0	39.0	35.0	39.0
13	37.96722456050217	39.0	38.0	39.0	35.0	39.0
14	39.33790450596426	40.0	39.0	41.0	36.0	41.0
15	39.356281410345076	40.0	39.0	41.0	37.0	41.0
16	39.3453474815737	40.0	39.0	41.0	36.0	41.0
17	39.33068547829352	40.0	39.0	41.0	36.0	41.0
18	39.32511971334664	40.0	39.0	41.0	36.0	41.0
19	39.36629978724946	40.0	39.0	41.0	37.0	41.0
20	39.34622351321622	40.0	39.0	41.0	37.0	41.0
21	39.31370495517748	40.0	39.0	41.0	36.0	41.0
22	39.27518590972264	40.0	39.0	41.0	36.0	41.0
23	39.241455398133326	40.0	39.0	41.0	36.0	41.0
24	39.186713300531544	40.0	39.0	41.0	36.0	41.0
25	39.14078421298766	40.0	39.0	41.0	36.0	41.0
26	39.04957812160373	40.0	39.0	41.0	36.0	41.0
27	38.981623095619184	40.0	39.0	41.0	35.0	41.0
28	38.91401057824675	40.0	38.0	41.0	35.0	41.0
29	38.85131174211736	40.0	38.0	41.0	35.0	41.0
30	38.780300485440094	40.0	38.0	41.0	35.0	41.0
31	38.70534379301941	40.0	38.0	41.0	35.0	41.0
32	38.595023086397795	40.0	38.0	41.0	35.0	41.0
33	38.517912541743236	40.0	38.0	41.0	35.0	41.0
34	38.45102456181951	40.0	38.0	41.0	34.0	41.0
35	38.426462742308374	40.0	38.0	41.0	34.0	41.0
36	38.380276773305404	40.0	38.0	41.0	34.0	41.0
37	38.33529617114892	40.0	38.0	41.0	34.0	41.0
38	38.277043360272955	40.0	38.0	41.0	34.0	41.0
39	38.20445129461669	40.0	38.0	41.0	34.0	41.0
40	38.13486935272459	40.0	38.0	41.0	34.0	41.0
41	38.09827362486086	40.0	38.0	41.0	34.0	41.0
42	38.03267005223256	40.0	37.0	41.0	34.0	41.0
43	37.19735082761937	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	1.0
18	4.0
19	7.0
20	7.0
21	25.0
22	30.0
23	83.0
24	120.0
25	177.0
26	259.0
27	374.0
28	568.0
29	806.0
30	1168.0
31	1486.0
32	2068.0
33	2851.0
34	3904.0
35	5781.0
36	9057.0
37	16920.0
38	40793.0
39	65330.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.95598764334314	19.183775630512248	13.025207316510892	25.835029409633712
2	19.420896977361497	20.70003490953162	34.1039777105934	25.775090402513484
3	20.295611279072066	21.966658103951364	30.070280132524484	27.667450484452083
4	15.810724471581667	15.8258738909637	33.85038960354628	34.51301203390835
5	16.121616904117346	34.724445234848936	33.20752728542165	15.946410575612068
6	34.56175364409403	34.03613465857819	15.528154866586307	15.873956830741465
7	29.1540695951153	29.545319817416566	21.099189176727855	20.20142141074028
8	26.900099459231598	32.59167045402151	20.157949163817918	20.350280922928977
9	26.36460041759704	14.419612570066064	20.23369626072809	38.9820907516088
10	17.705719235151925	26.315858807411356	32.53173144690128	23.446690510535433
11	35.74867771915611	21.505588818411155	21.081405075714162	21.66432838671857
12	21.919892504989427	25.399648270002174	28.40120931886893	24.279249906139466
13	30.792841570006786	19.46041720183637	24.18637737862351	25.56036384953333
14	23.19507841471206	21.40744692763188	24.34709295815467	31.05038169950139
15	26.11628167381324	27.311109793770292	21.797379809117317	24.775228723299147
16	25.63742828725934	25.93514731163673	23.5672271951838	24.86019720592013
17	24.089552828660064	26.29148800231852	25.076241099716114	24.542718069305298
18	23.929495919536823	24.76930068962792	26.437054162467643	24.864149228367616
19	24.66325475395367	25.517550273018884	26.041851917718894	23.777343055308553
20	25.474736696504436	24.430085429551905	25.94371002693962	24.15146784700404
21	25.422043063871268	25.132228084388853	25.13288675479677	24.31284209694311
22	25.453000573043255	24.881274658973396	24.83582640082729	24.82989836715606
23	24.644153312124146	24.866783909999278	25.5182089434268	24.97085383444978
24	25.128276061941364	25.05713965788659	25.12761739153345	24.686966888638594
25	25.02684081912252	24.31152475612728	25.362104056751043	25.299530367999157
26	24.935944302830308	25.294919675143756	25.13025207316511	24.63888394886083
27	24.862173217143873	24.72055907944224	24.99785932117428	25.41940838223961
28	24.261465805125773	25.099953234401042	25.754671619868137	24.883909340605054
29	24.148174494964465	24.964267130370636	25.89892043920143	24.988637935463473
30	24.181108015360195	25.66114042194426	25.843592124936603	24.31415943775894
31	24.910914827329552	25.204681829259457	25.315997128197022	24.56840621521397
32	24.230508295953786	25.063726361965738	25.595932051560723	25.109833290519756
33	23.928178578720992	24.832533048787717	26.215740905408342	25.023547467082945
34	24.67181746925656	24.231825636769617	25.774431732105572	25.321925161868254
35	24.559184829503163	24.19625743474223	26.321128170674672	24.92342956507993
36	23.88207165016697	24.43469612240731	26.4936998175483	25.18953240987742
37	24.338530242851782	23.178611654514196	26.80722693171564	25.675631170918383
38	23.534293674788074	23.245796036121487	28.090316886333248	25.129593402757195
39	23.158192871868845	23.030410812733415	28.430190816817174	25.381205498580567
40	22.847959109741076	22.69646491592072	29.32927592362058	25.12630005071762
41	22.11551761614006	23.09166716066947	29.68298193267071	25.109833290519756
42	22.287430592605766	23.048853584155022	29.582864030667693	25.08085179257151
43	21.4489431633305	23.01987208620678	29.782441164265812	25.748743586196905
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14.0
1	13.5
2	13.0
3	36.5
4	60.0
5	60.0
6	63.5
7	67.0
8	75.0
9	83.0
10	132.5
11	182.0
12	182.0
13	302.5
14	423.0
15	578.5
16	734.0
17	723.0
18	712.0
19	712.0
20	896.5
21	1081.0
22	1158.0
23	1235.0
24	1504.5
25	1774.0
26	1774.0
27	1978.5
28	2183.0
29	2789.5
30	3396.0
31	3822.0
32	4248.0
33	4248.0
34	4735.0
35	5222.0
36	5955.0
37	6688.0
38	7158.0
39	7628.0
40	7628.0
41	8179.5
42	8731.0
43	8811.0
44	8891.0
45	9311.0
46	9731.0
47	9731.0
48	10151.0
49	10571.0
50	10785.0
51	10999.0
52	11539.0
53	12079.0
54	12079.0
55	11478.0
56	10877.0
57	10420.5
58	9964.0
59	9186.0
60	8408.0
61	8408.0
62	7796.0
63	7184.0
64	6425.5
65	5667.0
66	4876.5
67	4086.0
68	4086.0
69	3537.0
70	2988.0
71	2626.0
72	2264.0
73	1808.0
74	1352.0
75	1352.0
76	1127.5
77	903.0
78	737.0
79	571.0
80	450.0
81	329.0
82	329.0
83	268.0
84	207.0
85	171.5
86	136.0
87	109.5
88	83.0
89	83.0
90	63.5
91	44.0
92	26.0
93	8.0
94	5.5
95	3.0
96	3.0
97	2.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	151821.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.11368414567566
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.56315920879688	67.1196937683531
2	4.860576988955402	7.204705354503598
3	1.4346223318539646	3.1897543911407222
4	0.8134707758314773	2.411572645668474
5	0.44549030280578716	1.6508463796054758
6	0.3355903002091536	1.492310010723221
7	0.27066633486683256	1.4042055475831678
8	0.18776818006933724	1.1132953272213768
9	0.1608014271188931	1.0725827561697174
>10	0.9008874519611236	11.65924783345961
>50	0.02197286465501551	0.9963397608183431
>100	0.004993832876139888	0.6854462247531841
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	274	0.1804756917685959	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	257	0.169278294834048	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	181	0.11921934383253964	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	6.586704079145836E-4	0.0	0.0	0.0
10	0.0	6.586704079145836E-4	0.0	0.0	0.0
11	0.0	6.586704079145836E-4	0.0	0.0	0.0
12	0.0	6.586704079145836E-4	0.0	0.0	0.0
13	0.0	6.586704079145836E-4	0.0	0.0	0.0
14	0.0	6.586704079145836E-4	0.0	0.0	0.0
15	0.0	6.586704079145836E-4	0.0	6.586704079145836E-4	0.0
16	0.0	0.0013173408158291672	0.0	0.0019760112237437507	0.0
17	0.0	0.0013173408158291672	0.0	0.0019760112237437507	0.0
18	0.0	0.0013173408158291672	0.0	0.0019760112237437507	0.0
19	0.0	0.0013173408158291672	0.0	0.0019760112237437507	0.0
20	0.0	0.0013173408158291672	0.0	0.0019760112237437507	0.0
21	0.0	0.0013173408158291672	0.0	0.0019760112237437507	0.0
22	0.0	0.0013173408158291672	0.0	0.0019760112237437507	0.0
23	0.0	0.0013173408158291672	0.0	0.003293352039572918	0.0
24	0.0	0.0013173408158291672	0.0	0.007904044894975003	0.0
25	0.0	0.0013173408158291672	0.0	0.011197396934547922	0.0
26	0.0	0.0013173408158291672	0.0	0.015149419382035423	0.0
27	0.0	0.0013173408158291672	0.0	0.04215490610653335	0.0
28	0.0	0.0013173408158291672	0.0	0.11921934383253964	0.0
29	0.0	0.0013173408158291672	0.0	0.21999591624347092	0.0
30	0.0	0.0013173408158291672	0.0	0.3569993610897043	0.0
31	0.0	0.0013173408158291672	0.0	0.729148141561444	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGGC	25	0.0054815137	29.6	3
TTACACT	25	0.0054815137	29.6	4
TAGTTAT	35	8.833833E-4	26.42857	7
GGTATCA	130	3.092282E-11	19.923077	1
GGCGCAA	50	0.007007476	18.5	34
GTATCAA	145	1.5097612E-10	17.862068	2
GGAGTGG	90	4.405203E-5	16.444445	6
TCTCTTA	500	0.0	14.059999	37
CTCTTAT	295	0.0	13.79661	37
CTTATAC	135	6.47705E-6	13.703704	37
TCTTATA	190	6.9376256E-9	13.631579	37
TATCAAC	245	7.2759576E-12	13.591836	1
CAACGCA	245	7.2759576E-12	13.591836	4
TCAACGC	245	7.2759576E-12	13.591836	3
ATCAACG	250	1.0913936E-11	13.32	2
CAGGAGT	100	0.0018197839	12.950001	4
GTCTCTT	730	0.0	11.910958	37
AACGCAG	280	8.0035534E-11	11.892857	5
TTATTCC	125	7.1554456E-4	11.84	4
TTACAGG	120	0.0071141766	10.791667	3
>>END_MODULE