FastQCFastQC Report
Fri 10 Feb 2017
ERR1632255.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632255.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53022
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9741.8369733318245256No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8681.6370563162460865No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5210.9826109916638376No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3160.59597902757346No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT2390.45075628984195243Illumina PCR Primer Index 10 (95% over 22bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1820.3432537437290181No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.22443514013051188No Hit
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC1190.22443514013051188No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA1080.20368903474029648No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT900.1697408622835804No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC870.1640828335407944No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG710.13390668024593563No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC690.1301346610840783No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.12636264192222096No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA650.12259062276036362No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC650.12259062276036362Illumina PCR Primer Index 10 (95% over 23bp)
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG630.11881860359850627No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG620.1169325940175776No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA600.11316057485572027No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC590.11127456527479158No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA590.11127456527479158No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA570.10750254611293425No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.10561653653200559No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGGT200.001826141637.013
ATACACT200.001826141637.04
CTATCGG200.001826141637.012
CGAAATT303.553759E-430.83333213
TTGTCGG358.7577954E-426.42857222
TCCTTGT358.7577954E-426.42857219
TCCTAAG358.7577954E-426.4285722
CCTTGTC358.7577954E-426.42857220
TTCCTTG358.7577954E-426.42857218
CGGGTAA358.7577954E-426.42857226
GTCCTAA358.7577954E-426.4285721
GTAAGTT358.7577954E-426.42857229
AAGGTAG358.7577954E-426.4285726
CTTGTCG358.7577954E-426.42857221
ATTCCTT358.7577954E-426.42857217
AGGTAGC358.7577954E-426.4285727
TGTCGGG358.7577954E-426.42857223
TCGGGTA358.7577954E-426.42857225
TAGCGAA400.001907117723.12500210
GAAATTC400.001907117723.12500214
Produced by FastQC (version 0.11.5)