##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632255.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	53022
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.706103127003885	31.0	31.0	34.0	30.0	34.0
2	31.823695824374788	31.0	31.0	34.0	30.0	34.0
3	31.800761947870694	33.0	31.0	34.0	30.0	34.0
4	35.62545735732338	37.0	35.0	37.0	33.0	37.0
5	35.16149900041492	37.0	35.0	37.0	32.0	37.0
6	35.19540945268002	37.0	35.0	37.0	32.0	37.0
7	35.17498396891856	37.0	35.0	37.0	32.0	37.0
8	35.182622307721324	37.0	35.0	37.0	32.0	37.0
9	36.763852740371924	38.0	37.0	39.0	32.0	39.0
10	36.55048847648146	38.0	35.0	39.0	32.0	39.0
11	36.75544113764098	38.0	37.0	39.0	32.0	39.0
12	36.692203236392444	38.0	37.0	39.0	32.0	39.0
13	36.75604466070688	38.0	37.0	39.0	32.0	39.0
14	37.707121572177584	39.0	37.0	40.0	33.0	41.0
15	37.658820866810004	39.0	37.0	40.0	33.0	41.0
16	37.56938629248236	39.0	37.0	40.0	32.0	41.0
17	37.62677756403003	39.0	37.0	40.0	33.0	41.0
18	37.72832031986722	39.0	37.0	40.0	33.0	41.0
19	37.77824299347441	39.0	37.0	40.0	33.0	41.0
20	37.80064878729584	39.0	37.0	40.0	33.0	41.0
21	37.80032816566708	39.0	37.0	40.0	33.0	41.0
22	37.71428840858511	39.0	37.0	40.0	33.0	41.0
23	37.602448040436045	39.0	37.0	40.0	33.0	41.0
24	37.51655916412055	39.0	36.0	40.0	33.0	41.0
25	37.446267587039344	39.0	36.0	40.0	32.0	41.0
26	37.24599222964053	39.0	36.0	40.0	32.0	41.0
27	37.07032929727283	39.0	36.0	40.0	32.0	41.0
28	36.93702614009279	39.0	35.0	40.0	31.0	41.0
29	36.762381652898796	38.0	35.0	40.0	31.0	41.0
30	36.54820640488854	38.0	35.0	40.0	31.0	41.0
31	36.30883406887707	38.0	35.0	40.0	30.0	41.0
32	36.07898608124929	38.0	35.0	40.0	30.0	41.0
33	35.8419901173098	38.0	35.0	40.0	30.0	41.0
34	35.67739806118215	38.0	35.0	40.0	29.0	41.0
35	35.564652408434235	38.0	35.0	40.0	29.0	41.0
36	35.453641884500776	38.0	34.0	40.0	28.0	41.0
37	35.26326807740183	38.0	34.0	40.0	27.0	41.0
38	35.087209083022145	38.0	34.0	40.0	27.0	41.0
39	34.82718494209951	38.0	34.0	40.0	25.0	41.0
40	34.60686130285542	37.0	33.0	40.0	25.0	41.0
41	34.362962543849726	37.0	33.0	40.0	24.0	41.0
42	34.07427105729697	37.0	33.0	40.0	22.0	41.0
43	33.10173135679529	36.0	32.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	2.0
15	0.0
16	2.0
17	2.0
18	3.0
19	6.0
20	20.0
21	24.0
22	54.0
23	97.0
24	131.0
25	213.0
26	378.0
27	507.0
28	717.0
29	967.0
30	1253.0
31	1601.0
32	1980.0
33	2632.0
34	3763.0
35	5069.0
36	7621.0
37	10587.0
38	10809.0
39	4583.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.35977518765795	19.40138055901324	15.65387952170797	22.584964731620836
2	19.916261174606767	21.77209460224058	33.48987212855042	24.82177209460224
3	21.936177435781374	23.226207989136586	31.51899211647995	23.31862245860209
4	15.420014333672816	17.990645392478594	31.111614046999357	35.477726226849235
5	14.209196182716607	37.2713213383124	31.552940288936664	16.966542190034325
6	30.345894157142318	31.95466032967447	17.732262079891367	19.96718343329184
7	26.473916487495757	33.00705367583267	20.004903624910416	20.514126211761155
8	30.7457281882992	30.892836935611633	19.17317339972087	19.1882614763683
9	28.00158424804798	12.724906642525744	20.357587416544078	38.9159216928822
10	21.155369469276906	26.671947493493263	27.139677869563577	25.03300516766625
11	32.541209309343294	23.624156010712536	18.247142695484893	25.58749198445928
12	20.929048319565464	30.298743917619102	26.687035570140694	22.085172192674737
13	33.06929199200332	18.886499943419714	26.18158500245181	21.862623062125156
14	23.448757119686167	22.62457093282034	26.89826864320471	27.028403304288783
15	30.775904341594057	24.66711930896609	22.692467277733773	21.864509071706085
16	23.916487495756478	24.514352532910866	28.1279468899702	23.441213081362452
17	20.746105390215384	29.4764437403342	26.72286975217834	23.054581117272075
18	21.172343555505265	22.673607181924485	30.025272528384445	26.12877673418581
19	22.34166949568104	27.33770887556109	30.92678510806835	19.393836520689526
20	23.946663649051338	22.051224020218022	31.920712157217757	22.08140017351288
21	26.802082154577345	22.258685074120176	29.54811210440949	21.391120666892988
22	25.779864961714004	23.463845196333597	28.83520048281845	21.921089359133944
23	23.939119610727623	23.92968956282298	29.331221002602692	22.799969823846705
24	22.151182528007244	24.88778242993474	30.166723246954096	22.79431179510392
25	22.02670589566595	24.9047565161631	29.21240239900419	23.85613518916676
26	23.558145675380032	23.65621817358832	29.951718154728223	22.83391799630342
27	22.23793896872996	24.482290370035077	29.333107012183625	23.946663649051338
28	21.78341065972615	24.105088453849348	31.445437742823735	22.66606314360077
29	21.71551431481272	24.814228056278527	30.96639130926785	22.503866319640903
30	21.155369469276906	25.734600731771717	32.18475349854778	20.925276300403606
31	21.391120666892988	26.319263701859608	30.27611180264796	22.013503828599447
32	20.698955150692168	26.377729998868393	31.443551733242803	21.479763117196633
33	21.08558711478254	25.653502319791784	31.617064614688246	21.643845950737433
34	21.368488551921843	26.30040360605032	29.565086190637846	22.76602165138999
35	20.538644336313226	26.579533024027764	30.789106408660555	22.092716230998455
36	20.59522462374109	26.61913922522726	29.59903436309456	23.186601787937082
37	19.938893289577912	26.756817924635058	30.438308626607824	22.86598015917921
38	20.981856587831466	24.972652861076533	30.589189393082115	23.456301158009882
39	20.60088265248387	24.846290219154312	31.36622534042473	23.186601787937082
40	20.276489004564144	24.81045603711667	31.102183999094716	23.810870959224474
41	18.788427445211422	25.183885934140545	32.0942250386632	23.933461581984837
42	19.06944287276979	25.044321225151823	31.884877975180114	24.00135792689827
43	18.098147938591527	26.09671457131002	32.303572102146276	23.50156538795217
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	8.0
2	14.0
3	54.5
4	95.0
5	95.0
6	128.0
7	161.0
8	177.0
9	193.0
10	252.5
11	312.0
12	312.0
13	658.5
14	1005.0
15	1456.5
16	1908.0
17	1624.5
18	1341.0
19	1341.0
20	1498.0
21	1655.0
22	1149.0
23	643.0
24	574.5
25	506.0
26	506.0
27	529.0
28	552.0
29	627.0
30	702.0
31	760.0
32	818.0
33	818.0
34	873.0
35	928.0
36	963.0
37	998.0
38	1228.0
39	1458.0
40	1458.0
41	1775.5
42	2093.0
43	2470.5
44	2848.0
45	3384.5
46	3921.0
47	3921.0
48	4739.5
49	5558.0
50	5448.0
51	5338.0
52	4838.5
53	4339.0
54	4339.0
55	3937.0
56	3535.0
57	3337.0
58	3139.0
59	2893.0
60	2647.0
61	2647.0
62	2313.0
63	1979.0
64	1617.5
65	1256.0
66	1137.5
67	1019.0
68	1019.0
69	879.0
70	739.0
71	639.0
72	539.0
73	458.0
74	377.0
75	377.0
76	303.5
77	230.0
78	160.5
79	91.0
80	70.0
81	49.0
82	49.0
83	35.0
84	21.0
85	14.5
86	8.0
87	6.0
88	4.0
89	4.0
90	2.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	53022.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.61219870996945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.88247863247864	63.467994417411646
2	5.948183760683761	8.400286673456302
3	1.5251068376068375	3.230734412130814
4	0.734508547008547	2.0746105390215384
5	0.41132478632478636	1.4522273773150767
6	0.296474358974359	1.2560823808984949
7	0.19230769230769232	0.9505488287880501
8	0.1469017094017094	0.8298442156086152
9	0.12286324786324786	0.7808079665044698
>10	0.6757478632478633	9.203726754931916
>50	0.04006410256410256	1.8539474180528839
>100	0.016025641025641024	2.042548376145751
>500	0.008012820512820512	4.4566406397344505
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	974	1.8369733318245256	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	868	1.6370563162460865	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	521	0.9826109916638376	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	316	0.59597902757346	No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT	239	0.45075628984195243	Illumina PCR Primer Index 10 (95% over 22bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	182	0.3432537437290181	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	119	0.22443514013051188	No Hit
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC	119	0.22443514013051188	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	108	0.20368903474029648	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT	90	0.1697408622835804	No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC	87	0.1640828335407944	No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG	71	0.13390668024593563	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	69	0.1301346610840783	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	67	0.12636264192222096	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	65	0.12259062276036362	No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC	65	0.12259062276036362	Illumina PCR Primer Index 10 (95% over 23bp)
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	63	0.11881860359850627	No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG	62	0.1169325940175776	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	60	0.11316057485572027	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	59	0.11127456527479158	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	59	0.11127456527479158	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	57	0.10750254611293425	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56	0.10561653653200559	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0018860095809286712	0.0	0.0	0.0
3	0.0	0.0018860095809286712	0.0	0.0	0.0
4	0.0	0.0018860095809286712	0.0	0.0	0.0
5	0.0	0.0018860095809286712	0.0	0.0	0.0
6	0.0	0.0018860095809286712	0.0	0.0	0.0
7	0.0	0.0018860095809286712	0.0	0.0	0.0
8	0.0	0.0018860095809286712	0.0	0.0	0.0
9	0.0	0.0018860095809286712	0.0	0.0	0.0
10	0.0	0.0018860095809286712	0.0	0.0	0.0
11	0.0	0.0018860095809286712	0.0	0.0	0.0
12	0.0	0.0018860095809286712	0.0	0.0	0.0
13	0.0	0.0018860095809286712	0.0	0.0	0.0
14	0.0	0.0018860095809286712	0.0	0.0	0.0
15	0.0	0.0018860095809286712	0.0	0.0018860095809286712	0.0
16	0.0	0.0018860095809286712	0.0	0.0018860095809286712	0.0
17	0.0	0.0018860095809286712	0.0	0.005658028742786013	0.0
18	0.0	0.0018860095809286712	0.0	0.007544038323714685	0.0
19	0.0	0.0018860095809286712	0.0	0.009430047904643355	0.0
20	0.0	0.0018860095809286712	0.0	0.011316057485572026	0.0
21	0.0	0.0018860095809286712	0.0	0.01697408622835804	0.0
22	0.0	0.0018860095809286712	0.0	0.01886009580928671	0.0
23	0.0	0.0018860095809286712	0.0	0.035834182037644755	0.0
24	0.0	0.0018860095809286712	0.0	0.056580287427860136	0.0
25	0.0	0.0018860095809286712	0.0	0.062238316170646145	0.0
26	0.0	0.0018860095809286712	0.0	0.07544038323714684	0.0
27	0.0	0.0018860095809286712	0.0	0.09430047904643356	0.0
28	0.0	0.0018860095809286712	0.0	0.1508807664742937	0.0
29	0.0	0.0018860095809286712	0.0	0.25272528384444193	0.0
30	0.0	0.0018860095809286712	0.0	0.441326241937309	0.0
31	0.0	0.0018860095809286712	0.0	0.8807664742936894	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGGT	20	0.0018261416	37.0	13
ATACACT	20	0.0018261416	37.0	4
CTATCGG	20	0.0018261416	37.0	12
CGAAATT	30	3.553759E-4	30.833332	13
TTGTCGG	35	8.7577954E-4	26.428572	22
TCCTTGT	35	8.7577954E-4	26.428572	19
TCCTAAG	35	8.7577954E-4	26.428572	2
CCTTGTC	35	8.7577954E-4	26.428572	20
TTCCTTG	35	8.7577954E-4	26.428572	18
CGGGTAA	35	8.7577954E-4	26.428572	26
GTCCTAA	35	8.7577954E-4	26.428572	1
GTAAGTT	35	8.7577954E-4	26.428572	29
AAGGTAG	35	8.7577954E-4	26.428572	6
CTTGTCG	35	8.7577954E-4	26.428572	21
ATTCCTT	35	8.7577954E-4	26.428572	17
AGGTAGC	35	8.7577954E-4	26.428572	7
TGTCGGG	35	8.7577954E-4	26.428572	23
TCGGGTA	35	8.7577954E-4	26.428572	25
TAGCGAA	40	0.0019071177	23.125002	10
GAAATTC	40	0.0019071177	23.125002	14
>>END_MODULE