FastQCFastQC Report
Fri 10 Feb 2017
ERR1632254.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632254.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1452843
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5850.024.3504281
CTTATAC14550.020.34364337
TATGGTA904.447256E-516.4444454
GTACTAT803.383334E-416.18751
GTATCAA8850.016.0960452
TCTTATA23700.016.08016837
TACCGTC1353.9739461E-715.0740737
TAACGCA750.00410509914.815
GGTATAT750.00410509914.81
TCGTTTA1152.2105281E-514.4782630
ATAATAC908.2758674E-414.3888893
GTATACC908.2758674E-414.3888891
ACCGTCG1803.3323886E-914.3888898
TTAAGGT1304.4456174E-614.2307694
TAACACA1304.4456174E-614.2307694
TAGAACA1051.6561062E-414.0952384
GTATAGA1458.9138484E-714.0344831
TTAATAG1356.569353E-613.7037033
GTCTAAA950.001245350813.6315791
TTGCGCG3000.013.56666718