##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632254.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1452843
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.322397533663306	33.0	31.0	34.0	31.0	34.0
2	32.51236162475918	34.0	31.0	34.0	31.0	34.0
3	32.60439428073095	34.0	31.0	34.0	31.0	34.0
4	36.137465644945806	37.0	35.0	37.0	35.0	37.0
5	35.906030452017184	37.0	35.0	37.0	35.0	37.0
6	35.91441470275866	37.0	35.0	37.0	35.0	37.0
7	35.8875941860201	37.0	35.0	37.0	35.0	37.0
8	35.8658203260779	37.0	35.0	37.0	35.0	37.0
9	37.600771728259694	39.0	37.0	39.0	35.0	39.0
10	37.50080979156041	39.0	37.0	39.0	35.0	39.0
11	37.59178452179623	39.0	37.0	39.0	35.0	39.0
12	37.53167066228078	39.0	37.0	39.0	35.0	39.0
13	37.57406134042013	39.0	37.0	39.0	35.0	39.0
14	38.855949335200016	40.0	38.0	41.0	36.0	41.0
15	38.83912989910128	40.0	38.0	41.0	36.0	41.0
16	38.82916461035363	40.0	38.0	41.0	36.0	41.0
17	38.802878218775184	40.0	38.0	41.0	35.0	41.0
18	38.790895506259105	40.0	38.0	41.0	35.0	41.0
19	38.81280427410257	40.0	38.0	41.0	35.0	41.0
20	38.77751140350334	40.0	38.0	41.0	35.0	41.0
21	38.74130652795932	40.0	38.0	41.0	35.0	41.0
22	38.70290113935229	40.0	38.0	41.0	35.0	41.0
23	38.64912588627952	40.0	38.0	41.0	35.0	41.0
24	38.58976640972218	40.0	38.0	41.0	35.0	41.0
25	38.51984901327948	40.0	38.0	41.0	34.0	41.0
26	38.40667711514596	40.0	38.0	41.0	34.0	41.0
27	38.321633514426544	40.0	38.0	41.0	34.0	41.0
28	38.24849484768829	40.0	38.0	41.0	34.0	41.0
29	38.16562216289028	40.0	38.0	41.0	34.0	41.0
30	38.086790520379694	40.0	38.0	41.0	33.0	41.0
31	38.00594351901754	40.0	38.0	41.0	33.0	41.0
32	37.91369679999835	40.0	37.0	41.0	33.0	41.0
33	37.8352230764095	40.0	37.0	41.0	33.0	41.0
34	37.752645674721904	40.0	37.0	41.0	33.0	41.0
35	37.72075854032404	40.0	37.0	41.0	33.0	41.0
36	37.679672201332146	40.0	37.0	41.0	33.0	41.0
37	37.620071129502634	40.0	37.0	41.0	33.0	41.0
38	37.571153937486706	39.0	37.0	41.0	33.0	41.0
39	37.51576873757178	39.0	37.0	41.0	33.0	41.0
40	37.44172701386179	39.0	36.0	41.0	32.0	41.0
41	37.389143217815004	39.0	36.0	41.0	32.0	41.0
42	37.33019947785136	39.0	36.0	41.0	32.0	41.0
43	36.38481515208457	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	0.0
14	2.0
15	4.0
16	10.0
17	21.0
18	43.0
19	115.0
20	168.0
21	320.0
22	616.0
23	930.0
24	1583.0
25	2521.0
26	3770.0
27	5699.0
28	7985.0
29	11160.0
30	15280.0
31	20506.0
32	27752.0
33	37635.0
34	53687.0
35	78325.0
36	124269.0
37	219397.0
38	450628.0
39	390414.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.35100213856556	17.482618562363587	11.777735102829418	27.38864419624144
2	20.397730518713995	19.284533841578202	31.98377250673335	28.33396313297445
3	20.69617983498561	20.39979543557012	28.125613022191658	30.778411707252605
4	15.86909253098924	14.730703868208748	31.952661092767766	37.447542508034246
5	17.34089643547169	33.63708260286899	32.20568223820468	16.81633872345463
6	37.86259079611492	32.34575243161167	14.450219328585401	15.341437443687997
7	32.259576568149484	28.110470298580093	19.435066280389552	20.19488685288087
8	29.225112417515177	31.21975326996792	18.604212568047615	20.95092174446929
9	27.69315060195768	13.392362423193696	17.83035056093466	41.08413641391396
10	18.35373815339992	24.780241223587133	31.093655680620685	25.77236494239226
11	39.27740299536839	19.55235355781733	19.604940107086588	21.56530333972769
12	24.129379430537227	23.11350916788669	26.808264898547197	25.94884650302889
13	32.73147890033541	17.518616946221993	22.103902486366387	27.64600166707621
14	24.52370971949481	18.45629569058735	22.37709098643143	34.64290360348641
15	27.560445278670855	25.132378378117938	19.822926496531284	27.484249846679926
16	27.814498882535826	23.449264648692253	21.607014660221374	27.129221808550547
17	26.508989615533128	23.738077686301963	22.80473526733446	26.948197430830444
18	26.88776419750792	22.800536603060344	23.470257970062836	26.8414412293689
19	27.444052798547403	23.12982201105006	23.029742374089974	26.39638281631257
20	27.54117272134704	22.77493163404442	22.720073676233426	26.96382196837511
21	28.050518879190662	23.001177690913607	22.370827405301192	26.57747602459454
22	28.169113937293982	22.716012673083053	22.192900402865277	26.921972986757687
23	27.314857833915983	22.58550992777609	22.646424975031714	27.45320726327621
24	27.190412178053652	23.033459224430995	22.37846759766885	27.397660999846508
25	27.443158001243077	22.22752217548627	22.72668141017302	27.60263841309763
26	27.1587501195931	22.909426551939884	22.83908171770797	27.09274161075904
27	27.18125771332484	22.656749559312328	22.60829284375531	27.553699883607518
28	26.953015570161398	22.823594841287047	23.005238694063983	27.218150894487568
29	26.60789913294141	23.114059812381655	23.29384523998808	26.984195814688857
30	26.618705531155122	23.349666825665263	22.91341872452839	27.118208918651227
31	27.216154808193316	22.97213119380415	22.515853399162882	27.295860598839656
32	26.32562499870943	22.923123833752168	22.950587227938602	27.800663939599808
33	26.2976797905899	22.556807583475987	23.56916748747112	27.576345138462997
34	27.016959162139337	22.062948302053282	23.54383784070268	27.376254695104702
35	26.604595265971614	22.251957024950393	24.101227730732088	27.042219978345905
36	26.27771892764738	22.327532981884485	24.007274013778503	27.38747407668963
37	25.99269157094056	21.55504758600895	24.688765406860895	27.763495436189594
38	25.41430835954057	21.523867341481495	25.607102763340567	27.454721535637365
39	25.279606949959494	21.09463995765544	26.004943410953558	27.620809681431513
40	25.15337169948852	21.34366893050385	26.59137979809243	26.911579571915205
41	24.367877327419414	21.221976497116344	27.293176206926695	27.116969968537553
42	24.232900595590852	21.44271610903587	27.376943000723408	26.947440294649873
43	23.519127668991075	20.725363993218814	27.825098789063922	27.93040954872619
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	20.5
2	31.0
3	60.5
4	90.0
5	90.0
6	120.0
7	150.0
8	146.5
9	143.0
10	197.5
11	252.0
12	252.0
13	466.5
14	681.0
15	1058.0
16	1435.0
17	1503.5
18	1572.0
19	1572.0
20	1999.5
21	2427.0
22	2663.0
23	2899.0
24	3814.0
25	4729.0
26	4729.0
27	6197.5
28	7666.0
29	9980.5
30	12295.0
31	15366.0
32	18437.0
33	18437.0
34	23260.0
35	28083.0
36	33640.5
37	39198.0
38	45773.5
39	52349.0
40	52349.0
41	58985.0
42	65621.0
43	72026.0
44	78431.0
45	84555.5
46	90680.0
47	90680.0
48	96653.5
49	102627.0
50	108104.0
51	113581.0
52	117579.0
53	121577.0
54	121577.0
55	121369.5
56	121162.0
57	119811.5
58	118461.0
59	115478.5
60	112496.0
61	112496.0
62	104484.0
63	96472.0
64	86884.5
65	77297.0
66	68732.5
67	60168.0
68	60168.0
69	51829.0
70	43490.0
71	36953.5
72	30417.0
73	24471.5
74	18526.0
75	18526.0
76	15094.0
77	11662.0
78	9572.0
79	7482.0
80	6216.5
81	4951.0
82	4951.0
83	3721.0
84	2491.0
85	2001.0
86	1511.0
87	1148.5
88	786.0
89	786.0
90	586.0
91	386.0
92	232.0
93	78.0
94	52.0
95	26.0
96	26.0
97	20.0
98	14.0
99	8.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1452843.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.46166892951169
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.92774314348586	55.390457303755035
2	7.757948922169465	10.001806699859003
3	2.3144465493884376	4.475792616651846
4	1.1084791191574843	2.85817655977626
5	0.6307714037767442	2.0330288700229913
6	0.4377152574379061	1.692951360621497
7	0.31424232717158973	1.417960940044202
8	0.23016753314465768	1.1869586655914666
9	0.17197343491645173	0.9977125163629714
>10	0.996745380447638	11.89617699675977
>50	0.06958705108834586	3.044712611410431
>100	0.03911129606350706	4.588007530234075
>500	0.0010685817519154165	0.41625732891052686
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	6.883056187075961E-5	0.0
10	0.0	0.0	0.0	1.3766112374151922E-4	0.0
11	0.0	6.883056187075961E-5	0.0	1.3766112374151922E-4	0.0
12	0.0	6.883056187075961E-5	0.0	1.3766112374151922E-4	0.0
13	0.0	6.883056187075961E-5	0.0	1.3766112374151922E-4	0.0
14	0.0	6.883056187075961E-5	0.0	1.3766112374151922E-4	0.0
15	0.0	6.883056187075961E-5	0.0	1.3766112374151922E-4	0.0
16	0.0	6.883056187075961E-5	0.0	1.3766112374151922E-4	0.0
17	0.0	6.883056187075961E-5	0.0	2.7532224748303845E-4	0.0
18	0.0	6.883056187075961E-5	0.0	4.129833712245576E-4	0.0
19	0.0	6.883056187075961E-5	0.0	4.8181393309531726E-4	0.0
20	0.0	6.883056187075961E-5	0.0	4.8181393309531726E-4	0.0
21	0.0	6.883056187075961E-5	0.0	6.883056187075961E-4	0.0
22	0.0	6.883056187075961E-5	0.0	8.259667424491152E-4	0.0
23	0.0	6.883056187075961E-5	0.0	0.0015142723611567114	0.0
24	0.0	6.883056187075961E-5	0.0	0.002133747417993548	0.0
25	0.0	6.883056187075961E-5	0.0	0.0027532224748303843	0.0
26	0.0	6.883056187075961E-5	0.0	0.005093461578436211	0.0
27	0.0	6.883056187075961E-5	0.0	0.02085566024684016	0.0
28	0.0	6.883056187075961E-5	0.0	0.0925082751543009	0.0
29	0.0	6.883056187075961E-5	0.0	0.19520347346547426	0.0
30	0.0	6.883056187075961E-5	0.0	0.3466995401430161	0.0
31	0.0	6.883056187075961E-5	0.0	0.8577664620334062	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	585	0.0	24.350428	1
CTTATAC	1455	0.0	20.343643	37
TATGGTA	90	4.447256E-5	16.444445	4
GTACTAT	80	3.383334E-4	16.1875	1
GTATCAA	885	0.0	16.096045	2
TCTTATA	2370	0.0	16.080168	37
TACCGTC	135	3.9739461E-7	15.074073	7
TAACGCA	75	0.004105099	14.8	15
GGTATAT	75	0.004105099	14.8	1
TCGTTTA	115	2.2105281E-5	14.47826	30
ATAATAC	90	8.2758674E-4	14.388889	3
GTATACC	90	8.2758674E-4	14.388889	1
ACCGTCG	180	3.3323886E-9	14.388889	8
TTAAGGT	130	4.4456174E-6	14.230769	4
TAACACA	130	4.4456174E-6	14.230769	4
TAGAACA	105	1.6561062E-4	14.095238	4
GTATAGA	145	8.9138484E-7	14.034483	1
TTAATAG	135	6.569353E-6	13.703703	3
GTCTAAA	95	0.0012453508	13.631579	1
TTGCGCG	300	0.0	13.566667	18
>>END_MODULE