FastQCFastQC Report
Fri 10 Feb 2017
ERR1632253.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632253.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187914
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA2090.11122109049884522No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAG352.3777675E-531.7142851
GCGAACA250.00548479329.624
ACATGTA250.00548479329.68
TATAATC250.00548479329.63
TTCTCAC250.00548479329.62
TTCTGAG405.914122E-527.7500022
TCTAGAC358.8416965E-426.428573
ACAACGG509.038353E-625.90000223
GGTATCA1250.025.161
AGCAATT601.3274839E-624.66666816
AATTAAA551.8917006E-523.54545437
TACAACG551.8917006E-523.54545422
CTAGACA400.00192521323.1250024
ATCGTTT400.00192521323.12500229
CATCGTT400.00192521323.12500228
CTAGAAC652.663066E-622.769233
TACCATG652.663066E-622.769232
TAGAACT652.663066E-622.769234
ATTACAA705.064545E-621.14285720
CTCTATG450.003814086320.5555551
Produced by FastQC (version 0.11.5)