##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632253.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	187914
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.470353459561288	31.0	31.0	34.0	30.0	34.0
2	31.66463382185468	31.0	31.0	34.0	30.0	34.0
3	31.748879806720094	31.0	31.0	34.0	30.0	34.0
4	35.540082165245806	37.0	35.0	37.0	33.0	37.0
5	34.768010898602554	35.0	35.0	37.0	32.0	37.0
6	34.703481379780115	35.0	35.0	37.0	31.0	37.0
7	34.692412486563	35.0	35.0	37.0	32.0	37.0
8	34.64807305469523	35.0	35.0	37.0	31.0	37.0
9	36.27513649861107	37.0	35.0	39.0	32.0	39.0
10	36.008865757740246	37.0	35.0	39.0	32.0	39.0
11	36.15673127068766	37.0	35.0	39.0	32.0	39.0
12	36.076173143033515	37.0	35.0	39.0	32.0	39.0
13	36.166773098332214	37.0	35.0	39.0	32.0	39.0
14	37.15575742094788	38.0	36.0	40.0	32.0	41.0
15	37.08863629106932	38.0	36.0	40.0	32.0	41.0
16	37.05957512479113	38.0	36.0	40.0	32.0	41.0
17	37.01821577955874	38.0	36.0	40.0	32.0	41.0
18	37.01619889949658	38.0	36.0	40.0	32.0	41.0
19	37.02951882244005	39.0	36.0	40.0	31.0	41.0
20	36.99605670679141	39.0	36.0	40.0	31.0	41.0
21	36.94699170897325	38.0	36.0	40.0	31.0	41.0
22	36.89711783049693	38.0	36.0	40.0	31.0	41.0
23	36.80853475526038	38.0	36.0	40.0	31.0	41.0
24	36.7087710335579	38.0	36.0	40.0	31.0	41.0
25	36.61167342507743	38.0	35.0	40.0	30.0	41.0
26	36.45687388911949	38.0	35.0	40.0	30.0	41.0
27	36.3300126653682	38.0	35.0	40.0	30.0	41.0
28	36.201278244303246	38.0	35.0	40.0	30.0	41.0
29	36.10525027406154	38.0	35.0	40.0	30.0	41.0
30	35.99411965047841	38.0	35.0	40.0	29.0	41.0
31	35.879849292761584	38.0	34.0	40.0	29.0	41.0
32	35.77046414849346	38.0	34.0	40.0	29.0	41.0
33	35.64794533669657	38.0	34.0	40.0	28.0	41.0
34	35.543413476377495	38.0	34.0	40.0	28.0	41.0
35	35.50541737177645	38.0	34.0	40.0	28.0	41.0
36	35.43124514405526	38.0	34.0	40.0	27.0	41.0
37	35.36556084166161	38.0	34.0	40.0	27.0	41.0
38	35.2807241610524	38.0	34.0	40.0	27.0	41.0
39	35.23753419117256	38.0	33.0	40.0	27.0	41.0
40	35.122641208212265	38.0	33.0	40.0	27.0	41.0
41	35.08624690017774	38.0	33.0	40.0	27.0	41.0
42	34.99881328692913	37.0	33.0	40.0	27.0	41.0
43	34.00311844780059	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	6.0
16	7.0
17	11.0
18	28.0
19	35.0
20	95.0
21	155.0
22	235.0
23	423.0
24	621.0
25	882.0
26	1358.0
27	1901.0
28	2533.0
29	3594.0
30	4908.0
31	6547.0
32	8776.0
33	11522.0
34	15692.0
35	21443.0
36	27930.0
37	34056.0
38	33257.0
39	11897.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.98455676532882	18.390327490234895	11.907042583309387	26.7180731611269
2	22.051044626797363	20.31088689506902	30.35803612290729	27.280032355226325
3	21.979735410879446	20.664240024692145	28.09849186329917	29.25753270112924
4	16.95882158859904	15.134050682759135	31.286652404823485	36.62047532381834
5	17.670849431122747	33.82770841980906	31.62989452621944	16.87154762284875
6	38.138190874549	32.19025724533563	13.902636312355652	15.76891556775972
7	31.662888342539674	27.880306948923444	19.621209702310633	20.835595006226253
8	28.980810370701494	30.350585906318848	19.053396766606	21.61520695637366
9	27.35613099609396	13.159743286822694	18.348819140670734	41.13530657641262
10	18.7154762284875	24.901816788530923	30.998222591185332	25.384484391796246
11	39.50477346019988	19.575444086124506	19.158764115499643	21.761018338175976
12	24.20628585416733	23.458603403684663	26.138552742211864	26.196557999936143
13	33.20774396798535	17.25044435220367	22.50657215534766	27.035239524463318
14	24.85072958906734	18.877784518449932	21.91906936151644	34.352416530966295
15	28.224081228647147	25.33339719233266	19.572783294485777	26.869738284534417
16	28.13201783794715	23.36441137967368	21.417776216780016	27.08579456559916
17	27.027789307874876	23.56450291090605	22.30115904083783	27.106548740381236
18	27.056525857573142	22.2623114829124	23.399533829304893	27.281628830209563
19	27.309301063252338	22.91207680108986	22.69442404504188	27.084198090615917
20	27.435422586928066	22.52626201347425	22.893983417946508	27.14433198165118
21	28.418319018274318	22.704535053269048	22.60076417935864	26.276381749097993
22	28.556148025160443	22.44484178932916	22.15268686739679	26.84632331811361
23	27.242781272284127	22.354374873612397	22.725821386378875	27.677022467724598
24	27.28482178017604	22.827463626978297	22.13725427589216	27.7504603169535
25	27.432761795289334	22.347456815351705	22.567770363038413	27.65201102632055
26	27.83720212437604	22.661962387049396	22.38364358163841	27.11719190693615
27	27.324201496429218	22.408655023042456	22.86045744329853	27.4066860372298
28	27.06929765743904	22.363421565184076	23.247336547569635	27.319944229807252
29	26.750002660791637	22.62417914577945	23.373458071245356	27.25236012218355
30	26.045425034856372	23.39900167097715	23.3064061219494	27.249167172217074
31	27.7259810338772	22.844492693466158	22.2122886001043	27.217237672552336
32	26.646763945208978	22.84076758517194	22.411315814681185	28.1011526549379
33	26.275849590770246	22.55553072150026	23.5033047032153	27.665314984514193
34	27.135817448407252	22.35543919026789	23.18134891492917	27.327394446395694
35	26.84845195142459	22.453888480900837	23.876879849292763	26.820779718381814
36	26.58130847089626	22.56670604638292	23.552263269367902	27.29972221335292
37	26.651553370158688	22.01805081047713	24.166373979586407	27.164021839777767
38	25.695797013527468	21.57103781517077	25.300403376012433	27.432761795289334
39	26.11460561746331	21.284736634843597	25.300935534340173	27.29972221335292
40	25.31370733420607	21.69662718051875	26.053939568100304	26.935725917174878
41	25.549453473397403	21.23684238534649	26.213054908096257	27.00064923315985
42	25.082218461636707	21.538576157178284	26.787253743733835	26.59195163745117
43	24.00353353129623	21.08038783698926	26.889428142661004	28.0266504890535
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	2.5
4	3.0
5	3.0
6	9.5
7	16.0
8	15.5
9	15.0
10	18.5
11	22.0
12	22.0
13	42.5
14	63.0
15	85.0
16	107.0
17	114.0
18	121.0
19	121.0
20	155.5
21	190.0
22	213.0
23	236.0
24	324.5
25	413.0
26	413.0
27	547.0
28	681.0
29	968.5
30	1256.0
31	1639.5
32	2023.0
33	2023.0
34	2627.5
35	3232.0
36	3990.0
37	4748.0
38	5632.0
39	6516.0
40	6516.0
41	7227.5
42	7939.0
43	9020.0
44	10101.0
45	11197.5
46	12294.0
47	12294.0
48	13077.5
49	13861.0
50	14737.0
51	15613.0
52	15966.5
53	16320.0
54	16320.0
55	16087.0
56	15854.0
57	15786.0
58	15718.0
59	15212.5
60	14707.0
61	14707.0
62	13860.0
63	13013.0
64	11813.5
65	10614.0
66	9315.0
67	8016.0
68	8016.0
69	6670.5
70	5325.0
71	4546.5
72	3768.0
73	2879.0
74	1990.0
75	1990.0
76	1600.5
77	1211.0
78	1007.0
79	803.0
80	661.0
81	519.0
82	519.0
83	403.0
84	287.0
85	227.5
86	168.0
87	127.5
88	87.0
89	87.0
90	66.5
91	46.0
92	29.0
93	12.0
94	6.5
95	1.0
96	1.0
97	2.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	187914.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.755624173708
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.07533697966792	57.46012846039983
2	7.711375031969399	10.295553069933296
3	2.343637966716845	4.693530459161487
4	1.046335991638594	2.7939524886900027
5	0.643614833230823	2.1482454959890287
6	0.3545455432844706	1.4200745423976744
7	0.28786447729202014	1.34516010013469
8	0.21338547823840387	1.1395744631527869
9	0.13795016549845815	0.8288054462444304
>10	1.1115213704758555	13.87743805829407
>50	0.06153058724276404	2.8653213991517466
>100	0.012901574744450528	1.1322160164509443
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	209	0.11122109049884522	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	5.321583277456709E-4
4	0.0	0.0	0.0	0.0	5.321583277456709E-4
5	0.0	0.0	0.0	0.0	5.321583277456709E-4
6	0.0	0.0	0.0	0.0	5.321583277456709E-4
7	0.0	0.0	0.0	0.0	5.321583277456709E-4
8	0.0	0.0	0.0	0.0	5.321583277456709E-4
9	0.0	0.0	0.0	0.0	5.321583277456709E-4
10	0.0	0.0	0.0	0.0	5.321583277456709E-4
11	0.0	0.0	0.0	0.0	5.321583277456709E-4
12	0.0	0.0	0.0	0.0	5.321583277456709E-4
13	0.0	0.0	0.0	0.0	5.321583277456709E-4
14	0.0	0.0	0.0	0.0	5.321583277456709E-4
15	0.0	0.0	0.0	0.0	5.321583277456709E-4
16	0.0	0.0	0.0	5.321583277456709E-4	5.321583277456709E-4
17	0.0	0.0	0.0	5.321583277456709E-4	5.321583277456709E-4
18	0.0	0.0	0.0	5.321583277456709E-4	5.321583277456709E-4
19	0.0	0.0	0.0	5.321583277456709E-4	5.321583277456709E-4
20	0.0	0.0	0.0	5.321583277456709E-4	5.321583277456709E-4
21	0.0	0.0	0.0	5.321583277456709E-4	5.321583277456709E-4
22	0.0	0.0	0.0	5.321583277456709E-4	5.321583277456709E-4
23	0.0	0.0	0.0	5.321583277456709E-4	5.321583277456709E-4
24	0.0	0.0	0.0	0.0010643166554913418	5.321583277456709E-4
25	0.0	0.0	0.0	0.0026607916387283545	5.321583277456709E-4
26	0.0	0.0	0.0	0.004257266621965367	5.321583277456709E-4
27	0.0	0.0	0.0	0.020754174782081164	5.321583277456709E-4
28	0.0	0.0	0.0	0.06705194929595454	5.321583277456709E-4
29	0.0	0.0	0.0	0.13250742360867204	5.321583277456709E-4
30	0.0	0.0	0.0	0.2251029726364188	5.321583277456709E-4
31	0.0	0.0	0.0	0.43956277871792415	5.321583277456709E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCTAG	35	2.3777675E-5	31.714285	1
GCGAACA	25	0.005484793	29.6	24
ACATGTA	25	0.005484793	29.6	8
TATAATC	25	0.005484793	29.6	3
TTCTCAC	25	0.005484793	29.6	2
TTCTGAG	40	5.914122E-5	27.750002	2
TCTAGAC	35	8.8416965E-4	26.42857	3
ACAACGG	50	9.038353E-6	25.900002	23
GGTATCA	125	0.0	25.16	1
AGCAATT	60	1.3274839E-6	24.666668	16
AATTAAA	55	1.8917006E-5	23.545454	37
TACAACG	55	1.8917006E-5	23.545454	22
CTAGACA	40	0.001925213	23.125002	4
ATCGTTT	40	0.001925213	23.125002	29
CATCGTT	40	0.001925213	23.125002	28
CTAGAAC	65	2.663066E-6	22.76923	3
TACCATG	65	2.663066E-6	22.76923	2
TAGAACT	65	2.663066E-6	22.76923	4
ATTACAA	70	5.064545E-6	21.142857	20
CTCTATG	45	0.0038140863	20.555555	1
>>END_MODULE