FastQCFastQC Report
Fri 10 Feb 2017
ERR1632252.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632252.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123507
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA2820.22832713935242538No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC2300.18622426259240366No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT2250.1821759090577862No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT1930.15626644643623439No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC1830.14816973936699943No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG1790.14493105653930546No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC1790.14493105653930546No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC1780.144121385832382No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT1760.14250204441853498No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC1630.13197632522852956No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG1620.13116665452160606No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA1580.1279279716939121No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC1570.1271183009869886No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC1500.12145060603852413No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC1500.12145060603852413No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC1500.12145060603852413No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC1480.11983126462467714No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG1450.11740225250390665No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT1400.1133538989692892No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATG1360.11011521614159522No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC1320.10687653331390125No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC1290.10444752119313076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1280.10363785048620726No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTTGT251.2272561E-437.034
TAATGAC200.001835582837.030
TAATAAT250.005477602629.627
ATAATGA250.005477602629.629
AAAAAAC250.005477602629.631
ATGACTT250.005477602629.632
TGAGGGT250.005477602629.621
GGTATCA1200.029.2916681
CCATAGT358.82446E-426.428572
ATAGTAG358.82446E-426.428574
CGCGTAA451.3138933E-424.66666610
GTGTTGG400.001921496123.1256
CATAGTA400.001921496123.1253
CAAGACG400.001921496123.1254
CGCAAGA400.001921496123.1252
GTTGGGT400.001921496123.1258
GGCCTAA400.001921496123.12510
TAGTAGG400.001921496123.1255
GTATCAA1550.022.6774182
GTCGCGT502.6832265E-422.28
Produced by FastQC (version 0.11.5)