##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632251.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	424631
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.273712941353786	33.0	31.0	34.0	31.0	34.0
2	32.4735382014031	34.0	31.0	34.0	31.0	34.0
3	32.55482524827438	34.0	31.0	34.0	31.0	34.0
4	36.111284856734436	37.0	35.0	37.0	35.0	37.0
5	35.834218415518414	37.0	35.0	37.0	35.0	37.0
6	35.841719045477134	37.0	35.0	37.0	35.0	37.0
7	35.82099045995229	37.0	35.0	37.0	35.0	37.0
8	35.80393094239469	37.0	35.0	37.0	35.0	37.0
9	37.50749945246579	39.0	37.0	39.0	35.0	39.0
10	37.40763627714416	39.0	37.0	39.0	35.0	39.0
11	37.494360986362274	39.0	37.0	39.0	35.0	39.0
12	37.45629970492027	39.0	37.0	39.0	35.0	39.0
13	37.49267010651601	39.0	37.0	39.0	35.0	39.0
14	38.753096217657216	40.0	38.0	41.0	36.0	41.0
15	38.74586876605806	40.0	38.0	41.0	35.0	41.0
16	38.721374558145776	40.0	38.0	41.0	35.0	41.0
17	38.70621080420412	40.0	38.0	41.0	35.0	41.0
18	38.688395336186005	40.0	38.0	41.0	35.0	41.0
19	38.726202279155316	40.0	38.0	41.0	35.0	41.0
20	38.694146211652	40.0	38.0	41.0	35.0	41.0
21	38.65684323565637	40.0	38.0	41.0	35.0	41.0
22	38.625597283288315	40.0	38.0	41.0	35.0	41.0
23	38.579882297806805	40.0	38.0	41.0	35.0	41.0
24	38.51920608716745	40.0	38.0	41.0	34.0	41.0
25	38.43990429337472	40.0	38.0	41.0	34.0	41.0
26	38.345288026545404	40.0	38.0	41.0	34.0	41.0
27	38.2500547534212	40.0	38.0	41.0	34.0	41.0
28	38.17638137582984	40.0	38.0	41.0	34.0	41.0
29	38.121472525557486	40.0	38.0	41.0	34.0	41.0
30	38.03636333663816	40.0	38.0	41.0	33.0	41.0
31	37.950592396692656	40.0	37.0	41.0	33.0	41.0
32	37.86350737463822	40.0	37.0	41.0	33.0	41.0
33	37.78095570036102	40.0	37.0	41.0	33.0	41.0
34	37.71662690665543	40.0	37.0	41.0	33.0	41.0
35	37.67766366562969	40.0	37.0	41.0	33.0	41.0
36	37.649342605697655	40.0	37.0	41.0	33.0	41.0
37	37.57773926067574	40.0	37.0	41.0	33.0	41.0
38	37.52881442946935	39.0	37.0	41.0	33.0	41.0
39	37.46565135376362	39.0	37.0	41.0	33.0	41.0
40	37.40483855394449	39.0	36.0	41.0	32.0	41.0
41	37.36081209332338	39.0	36.0	40.0	32.0	41.0
42	37.308689191321406	39.0	36.0	40.0	32.0	41.0
43	36.40595481724132	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	0.0
16	2.0
17	8.0
18	13.0
19	36.0
20	50.0
21	113.0
22	165.0
23	280.0
24	484.0
25	757.0
26	1164.0
27	1673.0
28	2376.0
29	3403.0
30	4575.0
31	6194.0
32	8387.0
33	11318.0
34	16041.0
35	23504.0
36	37398.0
37	67792.0
38	133751.0
39	105144.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.02990361042882	18.440716763495836	12.790399193652844	26.738980432422505
2	19.987471475233793	20.456584658209128	33.43467622476927	26.12126764178781
3	21.127284630655794	21.460044132434987	29.11916463941634	28.293506597492886
4	16.503269897864264	15.56975350363021	32.12082961441817	35.80614698408736
5	16.70838916612306	34.22430298306059	32.57487088790032	16.492436962916038
6	35.91565382649877	32.537191114167356	15.252536908515864	16.294618150818003
7	30.20010314838059	28.7411894091576	20.361914226705068	20.69679321575674
8	27.112481189550458	32.30451851136634	19.857240757269253	20.72575954181395
9	26.61251769183126	14.461968155881225	19.457128659942395	39.46838549234512
10	18.24007196836783	26.094185304417245	32.0169747380667	23.648767989148226
11	36.63816348782826	20.990224453702155	20.44245474305927	21.929157315410322
12	22.28758616304509	24.525529224197008	27.56864195030509	25.61824266245281
13	31.034239139393964	18.951042198991594	23.689038247325325	26.32568041428911
14	23.6744373350038	20.549135602440707	23.288219654240976	32.488207408314516
15	26.59603279082309	26.629709088596925	21.092195341366978	25.682062779213013
16	26.50583683244982	25.12039865200609	22.808509034903246	25.565255480640836
17	25.08860634291891	25.534876163068642	23.955387147900176	25.42113034611227
18	24.74454290901983	24.28108169210444	25.31374299097334	25.66063240790239
19	25.980203988875044	24.489262441979037	24.94942667869279	24.58110689045312
20	26.01882575695133	23.98929894425984	25.002884857676428	24.988990441112403
21	26.102663253507163	24.739126441545718	23.881911589120907	25.27629871582621
22	26.220177047836827	24.063245500210773	23.966691080020063	25.749886371932334
23	25.425369320657232	24.442869220570334	24.318761465837397	25.812999992935044
24	26.155885933904965	24.628442106205153	23.93183728931708	25.2838346705728
25	26.016706269678853	24.22197154706086	23.97328504042333	25.788037142836956
26	25.839140335962284	24.60606974055121	24.08184988849142	25.47294003499509
27	26.013880286648877	23.98270498385657	24.016145783044575	25.987268946449976
28	25.281715183300324	24.211374110698465	24.747839889221463	25.759070816779744
29	25.234850964719957	24.42143884925971	24.655524443575715	25.68818574244462
30	24.9084499247582	25.157842927153222	24.55426005166839	25.379447096420186
31	25.859628712929577	24.384701069870076	24.397182494919118	25.358487722281225
32	25.10226526089711	24.206899637567677	24.60654073772287	26.084294363812344
33	24.96002411505519	24.157209435957334	24.757495331240538	26.12527111774694
34	25.706083634967776	23.89180252972581	24.40660243835236	25.995511396954065
35	25.439970232978755	23.83763785498468	24.968266565559276	25.754125346477295
36	24.774451229420364	23.82845341013727	25.184689765937957	26.212405594504407
37	25.488011944488274	22.654728458355606	25.56054550892422	26.296714088231898
38	24.52788421005532	22.993375424780574	26.632770570212728	25.84596979495138
39	24.550256575709263	22.56005802685155	27.10117725743057	25.78850814000862
40	24.02933370385111	22.36082622323853	27.739142926446725	25.870697146463634
41	23.222044551622467	22.776245728644398	28.14161000963189	25.86009971010124
42	23.729308505502424	22.324559441020554	27.8088505078527	26.13728154562432
43	22.73856595491144	22.509896828069547	28.230392976490176	26.521144240528837
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	14.0
2	15.0
3	36.5
4	58.0
5	58.0
6	69.0
7	80.0
8	86.5
9	93.0
10	140.5
11	188.0
12	188.0
13	332.0
14	476.0
15	760.5
16	1045.0
17	1096.0
18	1147.0
19	1147.0
20	1491.5
21	1836.0
22	2099.0
23	2362.0
24	2849.0
25	3336.0
26	3336.0
27	3816.5
28	4297.0
29	5810.0
30	7323.0
31	8358.5
32	9394.0
33	9394.0
34	10781.0
35	12168.0
36	13667.0
37	15166.0
38	17564.5
39	19963.0
40	19963.0
41	21806.0
42	23649.0
43	23933.0
44	24217.0
45	25919.5
46	27622.0
47	27622.0
48	29043.0
49	30464.0
50	32365.0
51	34266.0
52	36116.0
53	37966.0
54	37966.0
55	34776.5
56	31587.0
57	30369.0
58	29151.0
59	27014.5
60	24878.0
61	24878.0
62	23173.0
63	21468.0
64	19473.0
65	17478.0
66	15505.0
67	13532.0
68	13532.0
69	11744.5
70	9957.0
71	8693.5
72	7430.0
73	6016.5
74	4603.0
75	4603.0
76	3775.5
77	2948.0
78	2403.5
79	1859.0
80	1516.5
81	1174.0
82	1174.0
83	914.5
84	655.0
85	531.5
86	408.0
87	313.5
88	219.0
89	219.0
90	168.0
91	117.0
92	66.0
93	15.0
94	11.0
95	7.0
96	7.0
97	4.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	424631.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.37460003303207
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.00619844631223	61.54137819260311
2	5.70932765794303	7.80745990138765
3	1.5340923158818485	3.1467884553650785
4	0.6797703861434341	1.859161130674283
5	0.37428889828642226	1.2795926858569173
6	0.2679150994980352	1.0991152662592891
7	0.18681386484888657	0.894132630276727
8	0.1329023911854131	0.7269718272588951
9	0.10964637250417891	0.6747324178541472
>10	0.8543193739131245	12.049142883211294
>50	0.10854389513400375	5.082531266075797
>100	0.03618129834951272	3.8389933431768517
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	2.354985858309921E-4	0.0
8	0.0	0.0	0.0	2.354985858309921E-4	0.0
9	0.0	0.0	0.0	2.354985858309921E-4	0.0
10	0.0	0.0	0.0	2.354985858309921E-4	0.0
11	0.0	0.0	0.0	2.354985858309921E-4	0.0
12	0.0	0.0	0.0	2.354985858309921E-4	0.0
13	0.0	0.0	0.0	2.354985858309921E-4	0.0
14	0.0	0.0	0.0	4.709971716619842E-4	0.0
15	0.0	0.0	0.0	4.709971716619842E-4	0.0
16	0.0	0.0	0.0	0.0011774929291549605	0.0
17	0.0	0.0	0.0	0.002354985858309921	0.0
18	0.0	0.0	0.0	0.002354985858309921	0.0
19	0.0	0.0	0.0	0.002825983029971905	0.0
20	0.0	0.0	0.0	0.003061481615802897	0.0
21	0.0	0.0	0.0	0.003296980201633889	0.0
22	0.0	0.0	0.0	0.004238974544957858	0.0
23	0.0	0.0	0.0	0.004945470302450834	0.0
24	0.0	0.0	0.0	0.006122963231605794	0.0
25	0.0	0.0	0.0	0.008006951918253732	0.0
26	0.0	0.0	0.0	0.010597436362394644	0.0
27	0.0	0.0	0.0	0.03367629777383187	0.0
28	0.0	0.0	0.0	0.08972496120160799	0.0
29	0.0	0.0	0.0	0.16814599028332836	0.0
30	0.0	0.0	0.0	0.27529784683642977	0.0
31	0.0	0.0	0.0	0.5390562629671409	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTGCGC	25	0.00549249	29.599998	8
CGTACCC	25	0.00549249	29.599998	3
ATGACGA	35	8.8601495E-4	26.42857	34
CTATATG	45	1.3212934E-4	24.666666	1
TATACTG	45	1.3212934E-4	24.666666	5
TACACAT	45	1.3212934E-4	24.666666	5
TTACACA	45	0.0038218955	20.555555	4
TAACCCG	55	5.135757E-4	20.181818	28
GCGGGTA	65	6.889038E-5	19.923077	23
GGTATCA	295	0.0	18.813559	1
CGGGTAA	70	1.21690115E-4	18.5	24
ACAGTAG	60	9.223746E-4	18.5	8
GTATGCC	50	0.007027757	18.499998	32
TAGGATA	50	0.007027757	18.499998	4
TGCGTAC	50	0.007027757	18.499998	16
TAGTCCT	85	2.7165732E-5	17.411764	4
TTAGGAC	130	1.385888E-8	17.076923	3
AACACTA	90	4.435343E-5	16.444445	5
TCTTATA	440	0.0	16.397728	37
GGGTAAC	80	3.376286E-4	16.1875	25
>>END_MODULE