FastQCFastQC Report
Fri 10 Feb 2017
ERR1632250.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632250.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1552899
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35630.22944183749232885No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28580.1840428772251125No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24450.15744745794800563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7100.023.971831
TAGACCG502.7026943E-422.25
CTTATAC9200.017.09239237
TCTTATA15450.016.64401237
GACGGAC2150.016.3488377
CGAACGT1659.749783E-1015.696974
AAGTCGT750.004105268514.819
CGTTATT1904.5474735E-1014.6052642
CGGTCCA2353.6379788E-1214.17021410
GTATCAA12550.014.1513941
ATACCGC2101.364242E-1014.09523927
GCGTTAT2252.7284841E-1113.97777751
TAACGCC2009.840733E-1013.8750014
ACGGACC2700.013.7037038
CGGACCA2700.013.7037039
TTATACT2202.8376235E-1013.4545454
CTCTTAT24200.013.378098537
ATACCGT1551.8885312E-613.1290326
AGACGGA2850.012.9824576
TATTCCG1000.001832984212.9500015
Produced by FastQC (version 0.11.5)