##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632250.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1552899
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.164248930548606	31.0	31.0	34.0	28.0	34.0
2	31.343491753166177	31.0	31.0	34.0	30.0	34.0
3	31.439977744850115	31.0	31.0	34.0	30.0	34.0
4	35.2707394363703	37.0	35.0	37.0	33.0	37.0
5	34.21767545732208	35.0	35.0	37.0	30.0	37.0
6	34.1492183329373	35.0	35.0	37.0	30.0	37.0
7	34.13554262060829	35.0	35.0	37.0	30.0	37.0
8	34.07517037489238	35.0	33.0	37.0	30.0	37.0
9	35.61901192543752	37.0	35.0	39.0	30.0	39.0
10	35.272375730810566	37.0	34.0	39.0	30.0	39.0
11	35.47440367982721	37.0	35.0	39.0	30.0	39.0
12	35.36836587569443	37.0	34.0	39.0	30.0	39.0
13	35.5024814878495	37.0	35.0	39.0	30.0	39.0
14	36.40022821831941	38.0	35.0	40.0	31.0	41.0
15	36.32951338110205	38.0	35.0	40.0	30.0	41.0
16	36.30394120931239	38.0	35.0	40.0	30.0	41.0
17	36.24472035850368	38.0	35.0	40.0	30.0	41.0
18	36.2285563967779	38.0	35.0	40.0	30.0	41.0
19	36.256048847993334	38.0	35.0	40.0	30.0	41.0
20	36.206159576379406	38.0	34.0	40.0	30.0	41.0
21	36.17084498090346	38.0	34.0	40.0	30.0	41.0
22	36.10972896498742	38.0	34.0	40.0	30.0	41.0
23	36.020879657981624	38.0	34.0	40.0	29.0	41.0
24	35.91216814486969	38.0	34.0	40.0	29.0	41.0
25	35.80979123561803	38.0	34.0	40.0	29.0	41.0
26	35.62687335106791	38.0	34.0	40.0	28.0	41.0
27	35.4797163241138	38.0	34.0	40.0	27.0	41.0
28	35.35546355558217	38.0	34.0	40.0	27.0	41.0
29	35.21470939191796	38.0	34.0	40.0	27.0	41.0
30	35.08824398753557	38.0	33.0	40.0	27.0	40.0
31	34.95600422178133	37.0	33.0	40.0	26.0	40.0
32	34.80349848895517	37.0	33.0	39.0	26.0	40.0
33	34.667315775204955	37.0	33.0	39.0	25.0	40.0
34	34.5586860446172	37.0	33.0	39.0	25.0	40.0
35	34.5111446397995	37.0	33.0	39.0	25.0	40.0
36	34.42393484701838	37.0	33.0	39.0	25.0	40.0
37	34.340528907546464	37.0	33.0	39.0	25.0	40.0
38	34.26766454225291	37.0	32.0	39.0	25.0	40.0
39	34.18055713861623	37.0	32.0	39.0	24.0	40.0
40	34.06372404129309	37.0	32.0	39.0	24.0	40.0
41	33.992130202930134	37.0	32.0	39.0	24.0	40.0
42	33.89495839716556	36.0	32.0	39.0	24.0	40.0
43	32.89526556459886	35.0	30.0	38.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	6.0
13	13.0
14	25.0
15	55.0
16	117.0
17	216.0
18	392.0
19	783.0
20	1211.0
21	2111.0
22	3542.0
23	5335.0
24	8166.0
25	12071.0
26	16959.0
27	23998.0
28	32793.0
29	44169.0
30	57656.0
31	74533.0
32	95844.0
33	121115.0
34	152383.0
35	187631.0
36	217633.0
37	230006.0
38	191246.0
39	72888.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.375582700484706	19.150118584660046	12.175936747979103	26.298361966876143
2	19.985652640641792	21.06305690196207	32.45987021692975	26.49142024046638
3	20.551883928059713	22.282711238786295	29.48330831560842	27.682096517545574
4	15.702631014637783	15.86368463113184	33.65769441541272	34.77598993881766
5	16.074194136257415	34.86099224740308	33.11078183449149	15.954031781848016
6	35.331016376467495	34.38072920389542	15.250444491238643	15.037809928398435
7	30.133318393533642	29.720928405517682	20.694069607875335	19.451683593073344
8	28.384138311635205	31.842315565918966	19.516852029655503	20.256694092790323
9	27.215034590143983	13.589035732523493	19.135500763410885	40.06042891392164
10	17.338796663530598	25.913726520527092	32.45465416617565	24.292822649766663
11	37.60508571388094	20.663095281792312	20.827497474079127	20.90432153024762
12	22.753765698863866	24.52619262424665	28.30081029094616	24.419231385943323
13	31.59072161164377	18.703405694768303	23.490709956024183	26.215162737563745
14	23.46958817025447	19.723948563299995	24.148576307924728	32.657886958520805
15	26.119535140405137	26.657239137896283	21.24864527570692	25.974580445991656
16	25.962087682457135	25.075745428389094	23.260366578895344	25.701800310258427
17	24.880497701395907	25.177039846120064	24.700704939600065	25.241757512883968
18	25.16931236352139	24.102211412332675	25.41182652574314	25.31664969840279
19	25.60243776317713	24.39817399586193	25.315361784636348	24.684026456324588
20	25.813591225185927	24.093453598720842	24.85589854845679	25.237056627636438
21	26.358121165639233	24.189596361386027	24.524904710480204	24.927377762494533
22	26.484851880257505	24.143682235612232	24.3186453207839	25.052820563346362
23	25.748229601538796	23.99306072062639	24.61351317761168	25.645196500223133
24	25.641075176170503	24.334164681669574	24.354706906244385	25.670053235915535
25	25.710815706623546	23.66110094732497	24.86710339822487	25.760979947826613
26	25.500950158381197	24.272344820880175	24.820674106944494	25.406030913794137
27	25.610422828529096	24.063702790715947	24.58678896695793	25.739085413797035
28	25.34633611071937	24.17053523764263	25.04245285752647	25.440675794111527
29	25.179551277964634	24.435909869218797	25.09641644434055	25.288122408476017
30	25.05848738391872	24.667991929932338	24.80843892616326	25.46508175998568
31	25.514730835682165	24.47564200891365	24.670438966088586	25.3391881893156
32	24.918491157506057	24.384264527184317	24.73625135955397	25.960992955755653
33	24.652408173358346	24.21831683837777	25.420262360913366	25.709012627350525
34	25.44241447769623	23.735993132843795	25.200351085292734	25.621241304167235
35	25.167316097183395	23.98230664067657	25.692140957010086	25.158236305129954
36	24.950302627537273	23.991708411171622	25.316392115649506	25.741596845641602
37	24.739020374151828	23.38864279003335	25.96653098495137	25.90580585086345
38	24.280973843115362	23.395275545930545	26.554592410710548	25.769158200243545
39	24.272280425191852	22.918039099774035	26.990744407717436	25.818936067316677
40	24.075422805990602	22.82221831555046	27.62137138345765	25.480987495001283
41	23.44730726209496	22.714162350545656	28.062353057088707	25.776177330270674
42	23.392120157202754	22.79884268068947	28.18084112360173	25.628196038506047
43	22.84997285721737	22.094418246131912	28.590268909954865	26.465339986695852
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	129.0
1	143.0
2	157.0
3	313.5
4	470.0
5	470.0
6	674.5
7	879.0
8	833.5
9	788.0
10	1108.5
11	1429.0
12	1429.0
13	2440.5
14	3452.0
15	5343.0
16	7234.0
17	7071.0
18	6908.0
19	6908.0
20	8099.5
21	9291.0
22	9220.0
23	9149.0
24	11086.0
25	13023.0
26	13023.0
27	15389.5
28	17756.0
29	21663.5
30	25571.0
31	30099.0
32	34627.0
33	34627.0
34	40442.5
35	46258.0
36	52800.5
37	59343.0
38	65296.0
39	71249.0
40	71249.0
41	77829.0
42	84409.0
43	89838.0
44	95267.0
45	98962.5
46	102658.0
47	102658.0
48	106316.0
49	109974.0
50	112456.0
51	114938.0
52	116061.0
53	117184.0
54	117184.0
55	114397.0
56	111610.0
57	108336.5
58	105063.0
59	101364.5
60	97666.0
61	97666.0
62	90843.5
63	84021.0
64	75268.5
65	66516.0
66	58284.5
67	50053.0
68	50053.0
69	43463.5
70	36874.0
71	31437.5
72	26001.0
73	21252.5
74	16504.0
75	16504.0
76	13324.0
77	10144.0
78	8340.0
79	6536.0
80	5454.5
81	4373.0
82	4373.0
83	3353.5
84	2334.0
85	1953.5
86	1573.0
87	1228.5
88	884.0
89	884.0
90	681.5
91	479.0
92	287.5
93	96.0
94	56.0
95	16.0
96	16.0
97	14.0
98	12.0
99	6.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1552899.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.31463473663317
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.61319017337202	58.97649894631714
2	7.021363780555284	9.452810764822091
3	2.0226018219763158	4.0845210859195324
4	0.9414512112833088	2.5349377763958714
5	0.5413607804619324	1.8220751598766813
6	0.3519759101499991	1.4215877896704723
7	0.2539834866399423	1.1967763942612986
8	0.176379951968687	0.9498361633309653
9	0.14039821328218194	0.8505769000289395
>10	0.8324003055814065	10.629435330677206
>50	0.0691647709818996	3.2207358952828518
>100	0.03505910859574651	4.095969311063529
>500	2.873507791519496E-4	0.12115111012757636
>1k	3.8313437220259945E-4	0.6430873722258896
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3563	0.22944183749232885	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2858	0.1840428772251125	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2445	0.15744745794800563	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	6.439568832229269E-5	0.0	0.0	0.0
10	0.0	6.439568832229269E-5	0.0	6.439568832229269E-5	0.0
11	0.0	1.2879137664458537E-4	0.0	1.2879137664458537E-4	0.0
12	0.0	1.2879137664458537E-4	0.0	2.5758275328917075E-4	0.0
13	0.0	1.2879137664458537E-4	0.0	2.5758275328917075E-4	0.0
14	0.0	1.2879137664458537E-4	0.0	3.863741299337562E-4	0.0
15	0.0	1.2879137664458537E-4	0.0	3.863741299337562E-4	0.0
16	0.0	1.2879137664458537E-4	0.0	6.43956883222927E-4	0.0
17	0.0	1.2879137664458537E-4	0.0	7.727482598675124E-4	0.0
18	0.0	1.2879137664458537E-4	0.0	8.37143948189805E-4	0.0
19	0.0	1.2879137664458537E-4	0.0	9.015396365120977E-4	0.0
20	0.0	1.2879137664458537E-4	0.0	0.001223518078123561	1.2879137664458537E-4
21	0.0	1.2879137664458537E-4	0.0	0.0015454965197350247	1.931870649668781E-4
22	0.0	1.2879137664458537E-4	0.0	0.0022538490912802443	1.931870649668781E-4
23	0.0	1.2879137664458537E-4	0.0	0.0030909930394700494	1.931870649668781E-4
24	0.0	1.2879137664458537E-4	0.0	0.004443302494238196	1.931870649668781E-4
25	0.0	1.2879137664458537E-4	0.0	0.00573121626068405	1.931870649668781E-4
26	0.0	1.2879137664458537E-4	0.0	0.008049461040286587	1.931870649668781E-4
27	0.0	1.931870649668781E-4	0.0	0.024985527069049565	1.931870649668781E-4
28	0.0	1.931870649668781E-4	0.0	0.08204010692260089	1.931870649668781E-4
29	0.0	1.931870649668781E-4	0.0	0.15738306225968335	1.931870649668781E-4
30	0.0	1.931870649668781E-4	0.0	0.26318517817321024	1.931870649668781E-4
31	0.0	1.931870649668781E-4	0.0	0.5425980698036382	1.931870649668781E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	710	0.0	23.97183	1
TAGACCG	50	2.7026943E-4	22.2	5
CTTATAC	920	0.0	17.092392	37
TCTTATA	1545	0.0	16.644012	37
GACGGAC	215	0.0	16.348837	7
CGAACGT	165	9.749783E-10	15.69697	4
AAGTCGT	75	0.0041052685	14.8	19
CGTTATT	190	4.5474735E-10	14.605264	2
CGGTCCA	235	3.6379788E-12	14.170214	10
GTATCAA	1255	0.0	14.151394	1
ATACCGC	210	1.364242E-10	14.095239	27
GCGTTAT	225	2.7284841E-11	13.9777775	1
TAACGCC	200	9.840733E-10	13.875001	4
ACGGACC	270	0.0	13.703703	8
CGGACCA	270	0.0	13.703703	9
TTATACT	220	2.8376235E-10	13.454545	4
CTCTTAT	2420	0.0	13.3780985	37
ATACCGT	155	1.8885312E-6	13.129032	6
AGACGGA	285	0.0	12.982457	6
TATTCCG	100	0.0018329842	12.950001	5
>>END_MODULE