FastQCFastQC Report
Fri 10 Feb 2017
ERR1632249.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632249.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373702
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41231.1032855055632562No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28140.7530064061739032No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27790.7436406548533323No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9490.25394565723490914No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA6130.16403444455742813No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA5260.1407538627034375No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT4930.13192329717261347No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA4220.11292420163659814No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT3950.10569919347501486No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTTG250.00549165729.59999816
CTGGCGC250.00549165729.59999824
GCGTAAA250.00549165729.59999814
GGTATCA6950.027.151081
AATAGGG400.001928762323.1253
TAGAGCA502.6973768E-422.1999994
TTATACC502.6973768E-422.1999994
GACTTAG603.7176294E-521.58333428
ATGCCGT705.086733E-621.14285934
GGTAGTT450.003821050520.55555520
TATGCCG759.238358E-619.73333433
TGCCGTC759.238358E-619.73333435
CACACTA951.668559E-719.47368410
CCGTCTT801.6119178E-518.537
GCCGTCT801.6119178E-518.536
TCGCCAT1002.8620525E-718.49999813
GTTTAAT500.00702621918.4999981
AGGTCGC1054.775884E-717.61904710
GTATATC752.0625662E-417.2666661
AGACTTA650.001577237717.07692327