##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632249.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	373702
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.882031672295035	34.0	31.0	34.0	31.0	34.0
2	33.04789377632445	34.0	33.0	34.0	31.0	34.0
3	33.11496325949553	34.0	33.0	34.0	31.0	34.0
4	36.482250563283046	37.0	37.0	37.0	35.0	37.0
5	36.383709479745896	37.0	37.0	37.0	35.0	37.0
6	36.41038046357793	37.0	37.0	37.0	35.0	37.0
7	36.405716319420286	37.0	37.0	37.0	35.0	37.0
8	36.40325446478745	37.0	37.0	37.0	35.0	37.0
9	38.16298280448058	39.0	39.0	39.0	37.0	39.0
10	38.16720006850378	39.0	38.0	39.0	37.0	39.0
11	38.20928440308053	39.0	39.0	39.0	37.0	39.0
12	38.204657721928164	39.0	39.0	39.0	37.0	39.0
13	38.19636769404499	39.0	39.0	39.0	37.0	39.0
14	39.60736094535218	41.0	39.0	41.0	37.0	41.0
15	39.62530840081134	41.0	39.0	41.0	37.0	41.0
16	39.62681762473843	41.0	39.0	41.0	37.0	41.0
17	39.66177863645365	41.0	39.0	41.0	38.0	41.0
18	39.67918287833621	41.0	40.0	41.0	38.0	41.0
19	39.73855906577968	41.0	40.0	41.0	38.0	41.0
20	39.71434993658048	41.0	40.0	41.0	38.0	41.0
21	39.70510727799155	41.0	40.0	41.0	37.0	41.0
22	39.65473023960268	41.0	40.0	41.0	37.0	41.0
23	39.61856505986053	41.0	39.0	41.0	37.0	41.0
24	39.588543812984675	41.0	39.0	41.0	37.0	41.0
25	39.545322208604716	41.0	39.0	41.0	37.0	41.0
26	39.46797715827049	41.0	39.0	41.0	37.0	41.0
27	39.40914686033256	41.0	39.0	41.0	36.0	41.0
28	39.36080620387367	41.0	39.0	41.0	36.0	41.0
29	39.29436823993449	40.0	39.0	41.0	36.0	41.0
30	39.21225736014257	40.0	39.0	41.0	36.0	41.0
31	39.10607114759889	40.0	39.0	41.0	35.0	41.0
32	39.00774949023553	40.0	38.0	41.0	35.0	41.0
33	38.93046331033818	40.0	38.0	41.0	35.0	41.0
34	38.85098554463182	40.0	38.0	41.0	35.0	41.0
35	38.82811170397804	40.0	38.0	41.0	35.0	41.0
36	38.783263134797245	40.0	38.0	41.0	35.0	41.0
37	38.71544439152051	40.0	38.0	41.0	35.0	41.0
38	38.656817464182694	40.0	38.0	41.0	35.0	41.0
39	38.59608458076221	40.0	38.0	41.0	35.0	41.0
40	38.51998918924705	40.0	38.0	41.0	35.0	41.0
41	38.44328101000262	40.0	38.0	41.0	35.0	41.0
42	38.36309144719589	40.0	38.0	41.0	35.0	41.0
43	37.640242760274226	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	0.0
13	0.0
14	1.0
15	2.0
16	2.0
17	3.0
18	7.0
19	7.0
20	19.0
21	25.0
22	57.0
23	108.0
24	157.0
25	280.0
26	409.0
27	643.0
28	981.0
29	1399.0
30	1953.0
31	2864.0
32	3677.0
33	5249.0
34	7233.0
35	10924.0
36	17804.0
37	38351.0
38	82432.0
39	199110.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.70544444503909	22.77697202583877	18.038169450524748	21.479414078597387
2	15.849794756249633	23.072662174673937	38.534447233357064	22.54309583571937
3	16.084473725053652	25.316161005292987	35.16464990821564	23.43471536143772
4	13.053716597716896	19.39165431279469	37.342320886695816	30.2123082027926
5	12.588907739321705	38.61151398708061	35.036740504466124	13.762837769131552
6	27.813605493146948	39.5400613322915	17.96618696180379	14.680146212757759
7	23.71327956500099	34.80955413671856	23.310819851111315	18.166346447169136
8	24.972304135380597	35.32306490198072	22.07213234074209	17.63249862189659
9	24.799974311082092	16.145752497979675	22.288614992694715	36.76565819824352
10	16.00366067080187	29.30944977548956	34.08892647082435	20.597963082884224
11	31.10580087877507	24.217959764732328	24.839042873733614	19.83719648275899
12	19.767889922986765	29.1507671888296	30.700130050146907	20.381212838036724
13	27.71486371493864	22.339190049825795	27.831801809998343	22.11414442523722
14	21.084714558659037	23.827274138217085	28.752321368363027	26.335689934760854
15	22.852433222193085	30.034091334806877	25.79006802211388	21.323407420886163
16	21.37210932775313	29.26904324836367	27.067021316450006	22.291826107433195
17	20.127802366591563	29.941504193180663	28.47616550085362	21.454527939374156
18	19.665401844250233	27.849195348165118	30.83472927626826	21.650673531316396
19	21.543101187577268	27.86872962949088	31.30649554993016	19.2816736330017
20	22.12779166287577	26.8946914921515	31.022044302679674	19.955472542293055
21	22.3603298885208	27.589362647242993	29.677657598835435	20.372649865400774
22	21.39378435223788	27.628431209894515	30.217392467795197	20.760391970072412
23	20.827290193790773	27.410878186362396	30.68835596277248	21.073475657074354
24	21.6718133700114	27.905657448983412	30.256728623341594	20.165800557663594
25	21.136895173159363	27.29420768419757	30.817870923891228	20.75102621875184
26	21.04698396048188	27.58508116092501	30.602458643518098	20.765476235075006
27	20.942622731481233	28.016708500355897	29.840621671813373	21.200047096349497
28	20.158307956607135	28.307849569978217	30.60112067904373	20.932721794370917
29	20.675565022397524	28.40097189739418	30.57569935403075	20.347763726177543
30	20.32582110879792	28.2505846904753	30.446184392912002	20.977409807814784
31	20.947706996483827	27.478311595870508	30.986454447661504	20.58752695998416
32	20.19791170504841	27.778818416813394	30.74508565648565	21.278184221652545
33	20.262401592712912	27.41917356610347	31.447517005528468	20.870907835655146
34	20.7836725519264	27.368866101867262	30.35172410102167	21.495737245184664
35	20.395127668570147	27.497578284301394	30.79030885571926	21.3169851914092
36	20.57227416497637	27.09271023435786	30.715918030944444	21.61909756972133
37	20.714098399259303	26.255947252088564	30.88985341261219	22.140100936039946
38	20.06358007182193	27.018051816688164	31.729292323830215	21.189075787659686
39	20.05020042707826	27.028220346693356	31.585327346388297	21.336251879840088
40	20.05100320576288	26.653322700975647	32.09214828927862	21.203525803982853
41	19.5155498231211	27.348529041856878	31.66132372853236	21.47459740648966
42	19.77377696667398	26.49945678642341	31.775585894643328	21.951180352259286
43	19.008193694441026	27.01377033037019	31.623593130355204	22.35444284483358
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	450.0
1	365.0
2	280.0
3	436.0
4	592.0
5	592.0
6	703.5
7	815.0
8	812.5
9	810.0
10	1179.0
11	1548.0
12	1548.0
13	2332.5
14	3117.0
15	5625.0
16	8133.0
17	7658.5
18	7184.0
19	7184.0
20	7760.5
21	8337.0
22	8220.0
23	8103.0
24	8938.0
25	9773.0
26	9773.0
27	10716.5
28	11660.0
29	14050.0
30	16440.0
31	17172.5
32	17905.0
33	17905.0
34	18709.0
35	19513.0
36	20682.5
37	21852.0
38	22763.5
39	23675.0
40	23675.0
41	25024.0
42	26373.0
43	24870.5
44	23368.0
45	23858.0
46	24348.0
47	24348.0
48	23439.5
49	22531.0
50	22612.0
51	22693.0
52	23165.0
53	23637.0
54	23637.0
55	20369.5
56	17102.0
57	15829.0
58	14556.0
59	12789.0
60	11022.0
61	11022.0
62	9849.0
63	8676.0
64	7533.0
65	6390.0
66	5426.0
67	4462.0
68	4462.0
69	3695.5
70	2929.0
71	2578.0
72	2227.0
73	1816.0
74	1405.0
75	1405.0
76	1052.0
77	699.0
78	573.0
79	447.0
80	357.0
81	267.0
82	267.0
83	212.0
84	157.0
85	131.0
86	105.0
87	93.5
88	82.0
89	82.0
90	56.5
91	31.0
92	19.0
93	7.0
94	4.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	373702.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.01516802088803
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.61011058930372	59.56046732375715
2	4.436717384731429	5.769078524590175
3	1.2424962283851808	2.4234330316134653
4	0.6201947259663075	1.6128825725747236
5	0.3754683990786263	1.2205570526315361
6	0.26566413161614266	1.0363318892488096
7	0.18631659912215914	0.847938349790533
8	0.140814271089256	0.7324050795685484
9	0.12000462874382424	0.7021908990957619
>10	0.8293115921764391	10.969510502952533
>50	0.11182886131812582	5.170154871727819
>100	0.05859667272010042	6.788192546536005
>500	0.0012379578743683187	0.5601828328656698
>1k	0.0012379578743683187	2.6066745230473414
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4123	1.1032855055632562	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2814	0.7530064061739032	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2779	0.7436406548533323	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	949	0.25394565723490914	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	613	0.16403444455742813	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	526	0.1407538627034375	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT	493	0.13192329717261347	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	422	0.11292420163659814	No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	395	0.10569919347501486	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.675928948734553E-4	0.0
3	0.0	0.0	0.0	2.675928948734553E-4	0.0
4	0.0	0.0	0.0	5.351857897469106E-4	0.0
5	0.0	0.0	0.0	5.351857897469106E-4	0.0
6	0.0	0.0	0.0	5.351857897469106E-4	0.0
7	0.0	0.0	0.0	0.0010703715794938213	0.0
8	0.0	0.0	0.0	0.0013379644743672767	0.0
9	0.0	0.0	0.0	0.0032111147384814636	0.0
10	0.0	0.0	0.0	0.0045490792128487405	0.0
11	0.0	0.0	0.0	0.005887043687216017	0.0
12	0.0	0.0	0.0	0.006422229476962927	0.0
13	0.0	0.0	0.0	0.006422229476962927	0.0
14	0.0	0.0	0.0	0.00856297263595057	0.0
15	0.0	0.0	0.0	0.010971308689811669	0.0
16	0.0	0.0	0.0	0.012576866059052401	0.0
17	0.0	0.0	0.0	0.014450016323166587	0.0
18	0.0	0.0	0.0	0.01659075948215423	0.0
19	0.0	0.0	0.0	0.018463909746268416	0.0
20	0.0	0.0	0.0	0.019534281325762237	0.0
21	0.0	0.0	0.0	0.023815767643737523	0.0
22	0.0	0.0	0.0	0.028900032646333174	0.0
23	0.0	0.0	0.0	0.036392633702789924	0.0
24	0.0	0.0	0.0	0.04923709265671578	0.0
25	0.0	0.0	0.0	0.0527158002900707	0.0
26	0.0	0.0	0.0	0.06368710897988236	0.0
27	0.0	0.0	0.0	0.10275567163140684	0.0
28	0.0	0.0	0.0	0.27909938935301387	0.0
29	0.0	0.0	0.0	0.5078913144698182	0.0
30	0.0	0.0	0.0	0.8686065367592359	0.0
31	0.0	0.0	0.0	1.366329321223863	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGTTG	25	0.005491657	29.599998	16
CTGGCGC	25	0.005491657	29.599998	24
GCGTAAA	25	0.005491657	29.599998	14
GGTATCA	695	0.0	27.15108	1
AATAGGG	40	0.0019287623	23.125	3
TAGAGCA	50	2.6973768E-4	22.199999	4
TTATACC	50	2.6973768E-4	22.199999	4
GACTTAG	60	3.7176294E-5	21.583334	28
ATGCCGT	70	5.086733E-6	21.142859	34
GGTAGTT	45	0.0038210505	20.555555	20
TATGCCG	75	9.238358E-6	19.733334	33
TGCCGTC	75	9.238358E-6	19.733334	35
CACACTA	95	1.668559E-7	19.473684	10
CCGTCTT	80	1.6119178E-5	18.5	37
GCCGTCT	80	1.6119178E-5	18.5	36
TCGCCAT	100	2.8620525E-7	18.499998	13
GTTTAAT	50	0.007026219	18.499998	1
AGGTCGC	105	4.775884E-7	17.619047	10
GTATATC	75	2.0625662E-4	17.266666	1
AGACTTA	65	0.0015772377	17.076923	27
>>END_MODULE