FastQCFastQC Report
Fri 10 Feb 2017
ERR1632248.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632248.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2760326
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33310.1206741522559292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13450.024.483271
CTTATAC27200.020.40441137
TCTTATA49900.017.01703537
TAGACCG700.00259372815.8571435
GTATCAA20850.015.7050362
TACCGTC2750.015.4727287
ACCGTCG2950.014.4237298
ATACCGT3100.014.3225816
CCGTCGT3250.013.6615389
GAACCGT1907.1377144E-913.6315796
ATACCAT2601.8189894E-1213.5192316
CGTCGTA3200.013.29687510
GTATTGG3550.013.0281691
GTACCGT1000.001833612112.9499996
CGCGGTT4000.012.94999910
GTATACG1000.001833612112.9499999
TATACAC8550.012.76608237
TATGCCG1602.6986927E-612.7187533
ATCAACG32200.012.6972062
CGCGCTT1755.312377E-712.68571412
Produced by FastQC (version 0.11.5)