##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632248.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2760326
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07228349115286	33.0	31.0	34.0	30.0	34.0
2	32.26721372765391	33.0	31.0	34.0	30.0	34.0
3	32.36229162787294	34.0	31.0	34.0	30.0	34.0
4	35.98806662691291	37.0	35.0	37.0	35.0	37.0
5	35.63474060672544	37.0	35.0	37.0	33.0	37.0
6	35.62334485129655	37.0	35.0	37.0	33.0	37.0
7	35.59763049726735	37.0	35.0	37.0	33.0	37.0
8	35.57088401877169	37.0	35.0	37.0	33.0	37.0
9	37.27347892966266	39.0	37.0	39.0	34.0	39.0
10	37.12833991347399	39.0	37.0	39.0	33.0	39.0
11	37.23510809955056	39.0	37.0	39.0	34.0	39.0
12	37.167612810950594	39.0	37.0	39.0	34.0	39.0
13	37.226142854141145	39.0	37.0	39.0	34.0	39.0
14	38.42003335837868	40.0	38.0	41.0	34.0	41.0
15	38.39217831517002	40.0	38.0	41.0	34.0	41.0
16	38.38040470582098	40.0	38.0	41.0	34.0	41.0
17	38.349092824543185	40.0	38.0	41.0	34.0	41.0
18	38.335612532722585	40.0	38.0	41.0	34.0	41.0
19	38.364012076834406	40.0	38.0	41.0	34.0	41.0
20	38.33760034140895	40.0	38.0	41.0	34.0	41.0
21	38.304724152147244	40.0	38.0	41.0	34.0	41.0
22	38.2599743653467	40.0	38.0	41.0	34.0	41.0
23	38.1948610417755	40.0	38.0	41.0	34.0	41.0
24	38.13217496773932	40.0	38.0	41.0	34.0	41.0
25	38.058895217448956	40.0	38.0	41.0	33.0	41.0
26	37.93515150022135	40.0	37.0	41.0	33.0	41.0
27	37.84723036337012	40.0	37.0	41.0	33.0	41.0
28	37.76303451114107	40.0	37.0	41.0	33.0	41.0
29	37.67141960768402	39.0	37.0	41.0	33.0	41.0
30	37.58476824838805	39.0	37.0	40.0	33.0	41.0
31	37.496782988675974	39.0	37.0	40.0	32.0	41.0
32	37.39537069172265	39.0	37.0	40.0	32.0	41.0
33	37.30370506961859	39.0	36.0	40.0	32.0	41.0
34	37.21825284404813	39.0	36.0	40.0	31.0	41.0
35	37.18307837552521	39.0	36.0	40.0	31.0	41.0
36	37.14259366466135	39.0	36.0	40.0	31.0	41.0
37	37.0844961790745	39.0	36.0	40.0	31.0	41.0
38	37.03680833350843	39.0	36.0	40.0	31.0	41.0
39	36.989917857528425	39.0	36.0	40.0	31.0	41.0
40	36.91159920965857	39.0	35.0	40.0	31.0	41.0
41	36.86113524272133	39.0	35.0	40.0	31.0	41.0
42	36.803326491146336	39.0	35.0	40.0	31.0	41.0
43	35.85871994829596	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	4.0
11	0.0
12	3.0
13	0.0
14	6.0
15	14.0
16	29.0
17	70.0
18	101.0
19	236.0
20	478.0
21	914.0
22	1571.0
23	2577.0
24	4177.0
25	6435.0
26	9336.0
27	13715.0
28	19600.0
29	27123.0
30	37031.0
31	49338.0
32	66538.0
33	90824.0
34	129019.0
35	188268.0
36	296314.0
37	497562.0
38	811488.0
39	507553.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.50233486914227	18.259038968585596	12.473019491176043	26.765606671096094
2	19.207224074257894	20.06400693251449	33.650844139424116	27.0779248538035
3	19.963004369773714	21.367295022399528	29.254334451800258	29.415366156026497
4	15.323661045832992	15.360432064908276	33.523576563058135	35.7923303262006
5	16.4548317843617	34.39561124301985	32.991574183629034	16.15798278898942
6	36.023462446102386	33.91896464403118	15.215811465747162	14.84176144411928
7	31.06259912778418	29.381203524511236	20.260505462036004	19.295691885668575
8	28.43642381370896	32.43011876133471	19.298916142513605	19.834541282442725
9	27.544826227047096	13.944476123472372	18.740250245804297	39.770447403676236
10	17.69457665507625	25.731960645228135	31.76834910079462	24.805113598901
11	38.108397341473434	20.369659235901846	20.607348552308675	20.91459487031604
12	23.332751276479662	23.99473830264976	27.88192409157469	24.790586329295888
13	31.77523234574467	18.36018644174637	23.134622504733137	26.72995870777582
14	23.863087186078747	19.15864285595252	23.651336834852117	33.32693312311662
15	26.456222924393714	26.179625160216585	20.88035978359078	26.48379213179893
16	26.54367636286439	24.676433145940006	22.674495693624593	26.105394797571012
17	25.424134685540768	24.801816886845973	24.066577643365314	25.70747078424795
18	25.710042944202964	23.809651468703336	24.740556006790502	25.739749580303194
19	26.185711397856632	24.075888137850384	24.462943869673364	25.275456594619623
20	26.40506954613332	23.80255085812328	24.014989533844915	25.777390061898487
21	27.134910876468936	23.925905853149228	23.474872170895754	25.464311099486075
22	27.22711013119465	23.823816462258442	23.413756201260284	25.535317205286624
23	26.447673209613647	23.68723839140739	23.66861740243725	26.196470996541716
24	26.181943726936602	23.93749868674932	23.611087965696807	26.269469620617276
25	26.27555585825732	23.190086967988563	23.995897586009768	26.538459587744345
26	25.92585078718963	23.840734753793573	24.134685540765837	26.098728918250963
27	26.10140976102098	23.622499661271892	23.944563069724374	26.331527507982756
28	25.89176061088437	23.828344912883477	24.254345320081757	26.025549156150397
29	25.647876374022488	24.033067108740056	24.382047627707742	25.937008889529718
30	25.57524002599693	24.34944278320749	24.01201887023489	26.063298320560683
31	26.030765931270437	24.044515031920145	23.605979873391767	26.318739163417654
32	25.25665446762448	23.90130730935404	24.156059827716	26.685978395305483
33	25.304692271854844	23.508201567496013	24.811924388641053	26.375181772008087
34	25.982112257755063	23.00695642471215	24.909231735671803	26.10169958186098
35	25.622806871362297	23.15157702387327	25.349831867685197	25.87578423707924
36	25.199088803279036	23.24805113598901	25.135473128898546	26.417386931833413
37	25.035340028677773	22.474483086418054	25.65425243250254	26.83592445240164
38	24.43099112206312	22.368336203767235	26.562695855489533	26.637976818680116
39	24.411790491412972	21.890276728183554	26.99855017124789	26.699382609155585
40	24.167217930056086	21.967803802884152	27.799288924569055	26.065689342490707
41	23.442122415975504	21.88589318797852	28.404289928073712	26.267694467972262
42	23.288191322329318	21.995300555079364	28.513733522779557	26.20277459981176
43	22.570957198533797	21.348891399059386	28.970744759858075	27.109406642548745
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	72.0
1	68.0
2	64.0
3	201.0
4	338.0
5	338.0
6	487.5
7	637.0
8	616.5
9	596.0
10	874.5
11	1153.0
12	1153.0
13	1975.0
14	2797.0
15	4708.0
16	6619.0
17	6744.0
18	6869.0
19	6869.0
20	8254.5
21	9640.0
22	10386.5
23	11133.0
24	14104.0
25	17075.0
26	17075.0
27	21725.0
28	26375.0
29	32777.0
30	39179.0
31	46840.5
32	54502.0
33	54502.0
34	65504.5
35	76507.0
36	88739.0
37	100971.0
38	112929.5
39	124888.0
40	124888.0
41	135972.5
42	147057.0
43	156296.5
44	165536.0
45	174797.5
46	184059.0
47	184059.0
48	191634.0
49	199209.0
50	203938.0
51	208667.0
52	212139.0
53	215611.0
54	215611.0
55	212708.5
56	209806.0
57	204455.0
58	199104.0
59	191181.0
60	183258.0
61	183258.0
62	169242.0
63	155226.0
64	139048.0
65	122870.0
66	108487.5
67	94105.0
68	94105.0
69	81167.0
70	68229.0
71	58574.5
72	48920.0
73	39442.5
74	29965.0
75	29965.0
76	24479.5
77	18994.0
78	15621.5
79	12249.0
80	10241.0
81	8233.0
82	8233.0
83	6328.0
84	4423.0
85	3623.0
86	2823.0
87	2232.0
88	1641.0
89	1641.0
90	1197.5
91	754.0
92	440.5
93	127.0
94	81.5
95	36.0
96	36.0
97	22.0
98	8.0
99	4.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2760326.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.24595951255786
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.0060604708266	54.61295919330771
2	8.746512230055945	11.238561412165328
3	2.4972687637053617	4.813182836549703
4	1.1270647722035851	2.896374308920871
5	0.6513577867849375	2.0923552998987196
6	0.3917460412334067	1.5100860182571763
7	0.2752115447959638	1.2376860835044996
8	0.20353476190067332	1.0461028857975012
9	0.14899980294704665	0.8615371776763551
>10	0.8382044079004771	9.875395776050675
>50	0.06974985999656198	3.0853103020449564
>100	0.04113156834772502	5.0460793373198385
>500	0.002819633305174934	1.2548988367114167
>1k	3.383559966034641E-4	0.4294705317954162
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3331	0.1206741522559292	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.622760500027895E-5	0.0
3	0.0	0.0	0.0	3.622760500027895E-5	0.0
4	0.0	0.0	0.0	3.622760500027895E-5	0.0
5	0.0	3.622760500027895E-5	0.0	3.622760500027895E-5	0.0
6	0.0	3.622760500027895E-5	0.0	3.622760500027895E-5	0.0
7	0.0	3.622760500027895E-5	0.0	3.622760500027895E-5	0.0
8	0.0	3.622760500027895E-5	0.0	3.622760500027895E-5	0.0
9	0.0	3.622760500027895E-5	0.0	1.0868281500083685E-4	0.0
10	0.0	3.622760500027895E-5	0.0	2.173656300016737E-4	0.0
11	0.0	7.24552100005579E-5	0.0	2.5359323500195267E-4	0.0
12	0.0	7.24552100005579E-5	0.0	2.898208400022316E-4	0.0
13	0.0	7.24552100005579E-5	0.0	3.260484450025106E-4	0.0
14	0.0	7.24552100005579E-5	0.0	3.260484450025106E-4	3.622760500027895E-5
15	0.0	7.24552100005579E-5	0.0	3.6227605000278953E-4	3.622760500027895E-5
16	0.0	7.24552100005579E-5	0.0	3.9850365500306847E-4	3.622760500027895E-5
17	0.0	7.24552100005579E-5	0.0	5.796416800044632E-4	3.622760500027895E-5
18	0.0	1.449104200011158E-4	0.0	5.796416800044632E-4	3.622760500027895E-5
19	0.0	1.449104200011158E-4	0.0	6.158692850047421E-4	3.622760500027895E-5
20	0.0	1.449104200011158E-4	0.0	7.245521000055791E-4	3.622760500027895E-5
21	0.0	1.449104200011158E-4	0.0	8.694625200066948E-4	7.24552100005579E-5
22	0.0	1.449104200011158E-4	0.0	0.001412876595010879	7.24552100005579E-5
23	0.0	1.8113802500139477E-4	0.0	0.0019200630650147844	1.0868281500083685E-4
24	0.0	1.8113802500139477E-4	0.0	0.002970663610022874	1.449104200011158E-4
25	0.0	1.8113802500139477E-4	0.0	0.0041299469700318	1.449104200011158E-4
26	0.0	1.8113802500139477E-4	0.0	0.0067383345300518855	1.449104200011158E-4
27	0.0	1.8113802500139477E-4	0.0	0.023403032830180203	1.449104200011158E-4
28	0.0	2.173656300016737E-4	0.0	0.09911872728076321	1.449104200011158E-4
29	0.0	2.898208400022316E-4	0.0	0.19736799204151972	1.449104200011158E-4
30	0.0	2.898208400022316E-4	0.0	0.33880056196260877	1.449104200011158E-4
31	0.0	2.898208400022316E-4	0.0	0.8889167438918446	1.449104200011158E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1345	0.0	24.48327	1
CTTATAC	2720	0.0	20.404411	37
TCTTATA	4990	0.0	17.017035	37
TAGACCG	70	0.002593728	15.857143	5
GTATCAA	2085	0.0	15.705036	2
TACCGTC	275	0.0	15.472728	7
ACCGTCG	295	0.0	14.423729	8
ATACCGT	310	0.0	14.322581	6
CCGTCGT	325	0.0	13.661538	9
GAACCGT	190	7.1377144E-9	13.631579	6
ATACCAT	260	1.8189894E-12	13.519231	6
CGTCGTA	320	0.0	13.296875	10
GTATTGG	355	0.0	13.028169	1
GTACCGT	100	0.0018336121	12.949999	6
CGCGGTT	400	0.0	12.949999	10
GTATACG	100	0.0018336121	12.949999	9
TATACAC	855	0.0	12.766082	37
TATGCCG	160	2.6986927E-6	12.71875	33
ATCAACG	3220	0.0	12.697206	2
CGCGCTT	175	5.312377E-7	12.685714	12
>>END_MODULE