##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632247.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3595715
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.924155001161104	31.0	31.0	34.0	30.0	34.0
2	32.118540540615705	33.0	31.0	34.0	30.0	34.0
3	32.2211991217324	34.0	31.0	34.0	30.0	34.0
4	35.89788873701058	37.0	35.0	37.0	35.0	37.0
5	35.43967444583345	37.0	35.0	37.0	33.0	37.0
6	35.41071608845529	37.0	35.0	37.0	33.0	37.0
7	35.38564040809686	37.0	35.0	37.0	33.0	37.0
8	35.34933886584449	37.0	35.0	37.0	33.0	37.0
9	37.03309717260684	39.0	37.0	39.0	33.0	39.0
10	36.850636938689526	39.0	37.0	39.0	33.0	39.0
11	36.9742905096761	39.0	37.0	39.0	33.0	39.0
12	36.89538631398762	39.0	37.0	39.0	33.0	39.0
13	36.9668310753216	39.0	37.0	39.0	33.0	39.0
14	38.124364417090895	40.0	38.0	41.0	33.0	41.0
15	38.09016092765973	40.0	38.0	41.0	33.0	41.0
16	38.07340598462336	40.0	37.0	41.0	33.0	41.0
17	38.03165156304101	40.0	37.0	41.0	33.0	41.0
18	38.020306670578734	40.0	37.0	41.0	33.0	41.0
19	38.04685799625388	40.0	37.0	41.0	34.0	41.0
20	38.013833966262624	40.0	37.0	41.0	33.0	41.0
21	37.98252586759518	40.0	37.0	41.0	33.0	41.0
22	37.93944903864739	39.0	37.0	40.0	33.0	41.0
23	37.87805596383473	39.0	37.0	40.0	33.0	41.0
24	37.81512133191869	39.0	37.0	41.0	33.0	41.0
25	37.73496564660992	39.0	37.0	40.0	33.0	41.0
26	37.60370607792887	39.0	37.0	40.0	33.0	41.0
27	37.49891968634889	39.0	37.0	40.0	32.0	41.0
28	37.417855141466994	39.0	37.0	40.0	32.0	41.0
29	37.32546072199827	39.0	36.0	40.0	32.0	41.0
30	37.236922281104036	39.0	36.0	40.0	32.0	41.0
31	37.14234748860797	39.0	36.0	40.0	31.0	41.0
32	37.03286383931986	39.0	36.0	40.0	31.0	41.0
33	36.94257637215408	39.0	36.0	40.0	31.0	41.0
34	36.85538954004976	39.0	36.0	40.0	31.0	41.0
35	36.83011028404643	39.0	36.0	40.0	31.0	41.0
36	36.78683182621537	39.0	36.0	40.0	31.0	41.0
37	36.72187033733208	39.0	35.0	40.0	30.0	41.0
38	36.67030840875876	38.0	35.0	40.0	30.0	41.0
39	36.61734536802833	38.0	35.0	40.0	30.0	41.0
40	36.537179114585	38.0	35.0	40.0	30.0	41.0
41	36.491546465723786	38.0	35.0	40.0	30.0	41.0
42	36.42836431697173	38.0	35.0	40.0	30.0	41.0
43	35.472262401219226	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	4.0
11	3.0
12	2.0
13	3.0
14	10.0
15	14.0
16	37.0
17	85.0
18	200.0
19	400.0
20	732.0
21	1336.0
22	2355.0
23	3960.0
24	6268.0
25	9587.0
26	14371.0
27	21086.0
28	29210.0
29	40793.0
30	55652.0
31	75155.0
32	101562.0
33	139009.0
34	197183.0
35	288599.0
36	443864.0
37	693447.0
38	968068.0
39	502717.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.121548843554066	17.718951585428766	11.885758465284372	27.273741105732796
2	19.186142394488996	19.7965077877418	33.20947294209914	27.80787687567007
3	20.040381398414503	21.20009511321114	28.95476977457891	29.804753713795446
4	15.336754998658126	15.109178563929564	33.41279828907464	36.14126814833767
5	16.45995302742292	34.473978054434234	33.016409810009975	16.049659108132875
6	36.55729110900057	33.773616652042776	15.05219406988596	14.616898169070685
7	31.779270604038416	29.09093184526582	19.925689327435574	19.204108223260185
8	29.190633851681792	32.335265726009986	18.910564380102425	19.563536042205794
9	27.93800398529917	13.70105806494675	17.872856997843268	40.48808095191082
10	17.474688622429753	25.649947228854348	31.893601133571487	24.981763015144416
11	38.72937649396573	20.10999203218275	20.36073493032679	20.799896543524724
12	23.60645935509349	23.483841183186097	28.022215331304068	24.887484130416343
13	32.259008291814006	18.051402850337137	22.826864754297823	26.862724103551034
14	23.97175526981421	18.282483456002492	23.60957417370398	34.13618710047932
15	26.6196570084114	25.57560874540947	20.738656984772152	27.066077261406978
16	26.855354220231582	24.302593503656436	22.34907382815379	26.492978447958194
17	25.845290853140472	24.45666578135364	23.71125075263195	25.986792612873934
18	26.187086573880297	23.718731879473207	24.186622132176772	25.907559414469723
19	26.733709429139964	23.68911329179315	24.004989271952866	25.572188007114022
20	26.860582665756322	23.58184672589457	23.57592300835856	25.98164759999054
21	27.223792764443232	23.66858886202049	23.21388096665058	25.8937374068857
22	27.41490913490085	23.47366240094112	23.19875184768537	25.912676616472663
23	26.83254929826196	23.34537080942177	23.345426431182673	26.476653461133598
24	26.51188984666471	23.47254996572309	23.31875579683039	26.696804390781804
25	26.72450402771076	22.853674443052356	23.641473253581	26.780348275655886
26	26.368079783853837	23.40191032937816	23.699876102527593	26.530133784240405
27	26.413467140749475	23.461064072096928	23.535041014095945	26.59042777305765
28	26.454043215327133	23.485899188339452	23.695176063731413	26.364881532602002
29	26.184027377030716	23.829725103352185	23.77794124395287	26.208306275664228
30	26.189422687838164	23.892355206127295	23.42541052335905	26.49281158267549
31	26.521734898344278	23.610936906846067	23.388310808837744	26.479017385971915
32	25.873046111830334	23.694508602600596	23.688390208901428	26.744055076667646
33	25.884893546902354	23.406777233457046	24.256232765945022	26.452096453695578
34	26.356899809912633	22.945811889985716	24.197051212345805	26.500237087755842
35	25.951945579669133	23.156479309400215	24.619609729914636	26.271965381016017
36	25.95842551481416	23.122494413489388	24.27339207918314	26.645687992513313
37	25.68496112734185	22.488239473929386	24.8107539112527	27.016045487476063
38	25.2176827140082	22.605573578551137	25.403737504223777	26.773006203216887
39	25.127269541662784	22.2559908112851	25.796983353797504	26.81975629325461
40	25.00175903818851	22.063789816489905	26.571766672275192	26.36268447304639
41	24.496073798952363	21.786876879841703	27.09141297349762	26.625636347708316
42	24.119876019094953	21.948402473499705	27.322827309728385	26.608894197676957
43	23.645978616214023	21.322296121911776	27.77453163000961	27.257193631864595
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	34.0
1	41.5
2	49.0
3	158.5
4	268.0
5	268.0
6	357.5
7	447.0
8	462.5
9	478.0
10	695.5
11	913.0
12	913.0
13	1576.0
14	2239.0
15	3884.0
16	5529.0
17	5884.5
18	6240.0
19	6240.0
20	7764.0
21	9288.0
22	10564.0
23	11840.0
24	15500.0
25	19160.0
26	19160.0
27	24168.0
28	29176.0
29	36803.0
30	44430.0
31	53796.0
32	63162.0
33	63162.0
34	77159.5
35	91157.0
36	105376.5
37	119596.0
38	135196.0
39	150796.0
40	150796.0
41	166439.0
42	182082.0
43	195270.0
44	208458.0
45	221590.5
46	234723.0
47	234723.0
48	247734.5
49	260746.0
50	268397.5
51	276049.0
52	281270.0
53	286491.0
54	286491.0
55	283930.0
56	281369.0
57	275058.0
58	268747.0
59	259783.5
60	250820.0
61	250820.0
62	232741.5
63	214663.0
64	193201.5
65	171740.0
66	152090.5
67	132441.0
68	132441.0
69	113707.5
70	94974.0
71	81332.0
72	67690.0
73	55418.5
74	43147.0
75	43147.0
76	35385.5
77	27624.0
78	22632.5
79	17641.0
80	14198.5
81	10756.0
82	10756.0
83	8078.5
84	5401.0
85	4210.5
86	3020.0
87	2265.5
88	1511.0
89	1511.0
90	1083.0
91	655.0
92	388.5
93	122.0
94	75.5
95	29.0
96	29.0
97	20.5
98	12.0
99	7.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3595715.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.16637938575469
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.16877063428427	50.259661982776905
2	10.139340800456319	12.403735322443458
3	3.074048797133885	5.640853049274422
4	1.3986001470748781	3.4218922881981717
5	0.7760820448482671	2.3735064394830694
6	0.49485818157384237	1.816120996577419
7	0.34340664825516687	1.4703458931536164
8	0.23791645894636232	1.1641990712022823
9	0.1862319541912077	1.0252020927428906
>10	1.0539706789303696	11.877152187449132
>50	0.08343218167451419	3.562090864001218
>100	0.042113343207425165	4.391929109602107
>500	0.0010916706016807192	0.41391246832770495
>1k	1.3645882179183838E-4	0.17939823476767083
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.781088045075875E-5	0.0
3	0.0	0.0	0.0	5.56217609015175E-5	0.0
4	0.0	0.0	0.0	8.343264135227626E-5	0.0
5	0.0	8.343264135227626E-5	0.0	8.343264135227626E-5	0.0
6	0.0	1.11243521803035E-4	0.0	8.343264135227626E-5	0.0
7	0.0	1.11243521803035E-4	0.0	8.343264135227626E-5	0.0
8	0.0	1.11243521803035E-4	0.0	1.3905440225379375E-4	2.781088045075875E-5
9	0.0	1.11243521803035E-4	0.0	2.5029792405682874E-4	2.781088045075875E-5
10	0.0	1.11243521803035E-4	0.0	6.952720112689688E-4	2.781088045075875E-5
11	0.0	1.11243521803035E-4	0.0	6.952720112689688E-4	2.781088045075875E-5
12	0.0	1.11243521803035E-4	0.0	7.508937721704863E-4	2.781088045075875E-5
13	0.0	1.11243521803035E-4	0.0	7.508937721704863E-4	2.781088045075875E-5
14	0.0	1.11243521803035E-4	0.0	8.343264135227625E-4	5.56217609015175E-5
15	0.0	1.11243521803035E-4	0.0	8.8994817442428E-4	5.56217609015175E-5
16	0.0	1.3905440225379375E-4	0.0	9.177590548750387E-4	5.56217609015175E-5
17	0.0	1.3905440225379375E-4	0.0	9.455699353257976E-4	5.56217609015175E-5
18	0.0	1.9467616315531125E-4	0.0	0.001001191696227315	8.343264135227626E-5
19	0.0	1.9467616315531125E-4	0.0	0.0011958678593826262	8.343264135227626E-5
20	0.0	1.9467616315531125E-4	0.0	0.0013905440225379375	8.343264135227626E-5
21	0.0	1.9467616315531125E-4	0.0	0.00155740930524249	8.343264135227626E-5
22	0.0	1.9467616315531125E-4	0.0	0.0018355181097500775	8.343264135227626E-5
23	0.0	2.5029792405682874E-4	0.0	0.0025864118819205638	1.11243521803035E-4
24	0.0	2.781088045075875E-4	0.0	0.00333730565409105	1.11243521803035E-4
25	0.0	2.781088045075875E-4	0.0	0.003976955904458501	1.11243521803035E-4
26	0.0	2.781088045075875E-4	0.0	0.005256256405193404	1.3905440225379375E-4
27	0.0	2.781088045075875E-4	0.0	0.01738180028172422	1.3905440225379375E-4
28	0.0	3.33730565409105E-4	0.0	0.07127928659529467	1.3905440225379375E-4
29	0.0	4.1716320676138125E-4	0.0	0.15562968700244598	1.3905440225379375E-4
30	0.0	4.1716320676138125E-4	0.0	0.2658720171092537	1.3905440225379375E-4
31	0.0	4.1716320676138125E-4	0.0	0.6381206519426595	1.3905440225379375E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1275	0.0	23.070587	1
CTTATAC	2725	0.0	17.855045	37
TATACAC	875	0.0	15.8571415	37
GTATCAA	2085	0.0	15.261391	1
GTACTAG	150	8.116149E-8	14.8	1
TCTTATA	4440	0.0	14.458333	37
TACGTCG	120	3.3042015E-5	13.874999	21
CCGTTAA	95	0.0012459757	13.631579	16
TATACTG	375	0.0	13.32	5
ATAATAC	320	0.0	13.296876	3
TAGAGTG	480	0.0	13.104166	5
TCTATAC	285	0.0	12.982456	3
TAACGCC	305	0.0	12.737705	4
CCAATAC	480	0.0	12.718749	3
ATCAACG	3170	0.0	12.547318	2
GTATTAG	650	0.0	12.523077	1
CGTTATT	270	4.3655746E-11	12.333334	2
CTAACGC	105	0.0026454553	12.333334	3
ATAACGC	390	0.0	12.333334	3
GTTATAT	300	1.8189894E-12	12.333333	1
>>END_MODULE