FastQCFastQC Report
Fri 10 Feb 2017
ERR1632246.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632246.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156041
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT2000.12817144212098103TruSeq Adapter, Index 8 (95% over 22bp)
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA1940.1243262988573516No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG405.907779E-527.751
GGTATCA601.3250683E-624.6666661
TTGCATG400.00192375523.12525
CGGCCTT652.6582493E-622.7692324
TCGGCCT753.69806E-722.223
GAATACA450.003811224820.5555551
TATGCCG450.003811224820.55555533
TCTCCGA450.003811224820.5555551
GCCGTCT450.003811224820.55555536
CCGGCGT555.1157584E-420.18181826
AACGTTG500.007008323418.512
ATCTCGC801.6021258E-518.511
ACGTTGC500.007008323418.513
TGTATTC500.007008323418.53
ACGGGTT609.188127E-418.58
CCCACGA500.007008323418.59
GGAACGT500.007008323418.510
GAACGTT500.007008323418.511
TCTTATA1900.017.52631637
CTTATAC953.5661415E-617.52631637
Produced by FastQC (version 0.11.5)