##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632246.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	156041
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.855864804762852	31.0	31.0	34.0	30.0	34.0
2	32.06580962695702	33.0	31.0	34.0	30.0	34.0
3	32.157086919463474	34.0	31.0	34.0	30.0	34.0
4	35.839702385911394	37.0	35.0	37.0	35.0	37.0
5	35.364058164200436	37.0	35.0	37.0	33.0	37.0
6	35.35910433796246	37.0	35.0	37.0	32.0	37.0
7	35.32943905768356	37.0	35.0	37.0	32.0	37.0
8	35.30043386033158	37.0	35.0	37.0	32.0	37.0
9	36.979454117828006	39.0	37.0	39.0	33.0	39.0
10	36.80132144756827	38.0	37.0	39.0	33.0	39.0
11	36.91825225421524	39.0	37.0	39.0	33.0	39.0
12	36.85830006216315	38.0	37.0	39.0	33.0	39.0
13	36.928781538185476	39.0	37.0	39.0	33.0	39.0
14	38.068981870149514	39.0	37.0	40.0	33.0	41.0
15	38.036189206682856	39.0	37.0	40.0	33.0	41.0
16	37.97271229997244	39.0	37.0	40.0	33.0	41.0
17	37.96563082779526	39.0	37.0	40.0	33.0	41.0
18	37.93949667074679	39.0	37.0	40.0	33.0	41.0
19	37.98179324664672	40.0	37.0	40.0	33.0	41.0
20	37.958350689882785	39.0	37.0	40.0	33.0	41.0
21	37.93099890413417	39.0	37.0	40.0	33.0	41.0
22	37.86422799136125	39.0	37.0	40.0	33.0	41.0
23	37.80543575086035	39.0	37.0	40.0	33.0	41.0
24	37.73983119821073	39.0	37.0	40.0	33.0	41.0
25	37.64859876570901	39.0	37.0	40.0	33.0	41.0
26	37.52025429214117	39.0	37.0	40.0	32.0	41.0
27	37.42433078485782	39.0	37.0	40.0	32.0	41.0
28	37.33500169827161	39.0	36.0	40.0	32.0	41.0
29	37.25737466435103	39.0	36.0	40.0	32.0	41.0
30	37.154344050602084	39.0	36.0	40.0	31.0	41.0
31	37.070359713152314	39.0	36.0	40.0	31.0	41.0
32	36.97258412853032	39.0	36.0	40.0	31.0	41.0
33	36.88182593036446	39.0	36.0	40.0	31.0	41.0
34	36.813113220243395	39.0	36.0	40.0	31.0	41.0
35	36.78686370889702	39.0	36.0	40.0	31.0	41.0
36	36.73195506309239	39.0	36.0	40.0	30.0	41.0
37	36.7027191571446	38.0	35.0	40.0	30.0	41.0
38	36.63751834453765	38.0	35.0	40.0	30.0	41.0
39	36.59447837427343	38.0	35.0	40.0	30.0	41.0
40	36.532501073435824	38.0	35.0	40.0	30.0	41.0
41	36.47485596734192	38.0	35.0	40.0	30.0	41.0
42	36.45694400830551	38.0	35.0	40.0	30.0	41.0
43	35.518043334764585	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	4.0
17	7.0
18	9.0
19	21.0
20	30.0
21	57.0
22	124.0
23	183.0
24	293.0
25	457.0
26	609.0
27	950.0
28	1333.0
29	1849.0
30	2478.0
31	3324.0
32	4502.0
33	6134.0
34	8652.0
35	12655.0
36	19725.0
37	30344.0
38	41826.0
39	20474.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.62111239994617	18.74186912414045	13.169615677930802	26.46740279798258
2	20.013970687191186	20.677257900167266	33.37199838503983	25.936773027601724
3	20.76313276638832	21.720573439032048	29.46341025756051	28.05288353701912
4	16.288667721944876	15.730481091508	32.28830884190693	35.692542344640195
5	16.58089860998071	34.671015950935974	32.81188918297114	15.936196256112176
6	36.435295851731276	33.01375920431169	15.038996161265308	15.511948782691729
7	30.488140937317752	29.07569164514455	20.18443870521209	20.251728712325605
8	27.548528912273056	32.703584314378915	19.612794073352514	20.135092699995514
9	27.09480200716478	14.246896648957646	18.855941707628123	39.80235963624945
10	18.002960760312995	26.116213046571097	31.635916201511144	24.24490999160477
11	37.42606109932646	20.686870758326336	20.393999013079895	21.493069129267308
12	22.99972443139944	24.157753410962503	27.265910882396295	25.576611275241763
13	31.63463448708993	18.538717388378696	23.30028646317314	26.526361661358234
14	23.919995385828084	20.087669266410753	22.827974698957327	33.16436064880384
15	26.921129703090855	26.598778526156586	20.47154273556309	26.00854903518947
16	27.054428002896675	24.97548721169436	22.156356342243384	25.81372844316558
17	25.524701841182768	25.4375452605405	23.26247588774745	25.775277010529287
18	25.0985317961305	23.85014194987215	24.731320614453896	26.320005639543453
19	26.200165341160336	24.32950314340462	24.167366269121576	25.30296524631347
20	26.635948244371672	23.2297921700066	24.12058369274742	26.01367589287431
21	26.92817913240751	24.148781410014035	23.10033901346441	25.822700444114048
22	26.92177056030146	23.691850218852736	23.03240814914029	26.353971071705512
23	25.796425298479246	24.217993988759364	23.489339340301587	26.4962413724598
24	26.875628841137907	24.046885113527853	23.370139899129075	25.70734614620516
25	26.427028793714474	23.507924199409132	23.49767048403945	26.567376522836945
26	26.12198076146654	24.220557417601785	23.512410199883362	26.145051621048314
27	26.207855627687593	23.40474618850174	23.688005075589107	26.699393108221557
28	25.44267211822534	23.93922110214623	24.45895629994681	26.15915047968162
29	25.499708409969173	24.209021987810896	24.372440576515146	25.91882902570478
30	25.353592965951254	24.928704635320205	24.08405483174294	25.6336475669856
31	26.196320197896704	24.20133170128364	23.670701930902776	25.931646169916885
32	25.19337866330003	23.65083535737402	24.012919681365794	27.14286629796015
33	25.025474074121547	23.601489352157444	24.402560865413577	26.970475708307433
34	25.95856217276229	23.282983318486806	24.485231445581608	26.273223063169294
35	25.75348786536872	23.274652174748944	25.179920661877325	25.791939298005012
36	24.98830435590646	23.65083535737402	25.176716375824306	26.184143910895212
37	25.51957498349793	21.932697175742273	25.70029671688851	26.84743112387129
38	24.278234566556225	22.02113547080575	27.032638857736107	26.667991104901915
39	24.163521125857947	21.64751571702309	27.560064342063946	26.62889881505502
40	23.83476137681763	21.38476426067508	28.51173729981223	26.268737062695063
41	22.751712690895342	21.833364308098513	29.262821950641175	26.15210105036497
42	23.124050730256794	21.509731416743037	29.067360501406682	26.29885735159349
43	21.658410289603374	21.32644625451003	29.799219435917486	27.215924019969112
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	2.0
2	1.0
3	1.5
4	2.0
5	2.0
6	5.5
7	9.0
8	16.0
9	23.0
10	26.5
11	30.0
12	30.0
13	41.0
14	52.0
15	99.0
16	146.0
17	166.5
18	187.0
19	187.0
20	244.0
21	301.0
22	415.5
23	530.0
24	696.5
25	863.0
26	863.0
27	1021.0
28	1179.0
29	1648.0
30	2117.0
31	2502.0
32	2887.0
33	2887.0
34	3462.0
35	4037.0
36	4573.0
37	5109.0
38	6072.0
39	7035.0
40	7035.0
41	7877.5
42	8720.0
43	8907.0
44	9094.0
45	9978.0
46	10862.0
47	10862.0
48	11355.5
49	11849.0
50	12608.5
51	13368.0
52	14226.5
53	15085.0
54	15085.0
55	13931.0
56	12777.0
57	12079.5
58	11382.0
59	10516.0
60	9650.0
61	9650.0
62	8974.0
63	8298.0
64	7351.0
65	6404.0
66	5630.0
67	4856.0
68	4856.0
69	4109.0
70	3362.0
71	2910.5
72	2459.0
73	1932.0
74	1405.0
75	1405.0
76	1111.0
77	817.0
78	661.5
79	506.0
80	398.5
81	291.0
82	291.0
83	224.0
84	157.0
85	128.0
86	99.0
87	76.5
88	54.0
89	54.0
90	42.5
91	31.0
92	16.5
93	2.0
94	1.5
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	156041.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.46740827683273
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.39081192333687	63.69606248276178
2	4.873729566506139	6.868781823877181
3	1.4562040198287873	3.0784476959892046
4	0.7635471725017077	2.152207613731962
5	0.4635937462814082	1.6334124896899196
6	0.3277003509213524	1.3855316652501777
7	0.2537666564195548	1.251759500947445
8	0.19382164363779258	1.0926487116088885
9	0.15385840391835412	0.9757802669162848
>10	1.0500336379159845	13.9066286712537
>50	0.06394115395686673	3.0934909316406403
>100	0.008991724775184382	0.8652481463328053
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT	200	0.12817144212098103	TruSeq Adapter, Index 8 (95% over 22bp)
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	194	0.1243262988573516	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0012817144212098103	0.0
21	0.0	0.0	0.0	0.0012817144212098103	0.0
22	0.0	0.0	0.0	0.0019225716318147154	0.0
23	0.0	0.0	0.0	0.0032042860530245255	0.0
24	0.0	0.0	0.0	0.003845143263629431	0.0
25	0.0	0.0	0.0	0.003845143263629431	0.0
26	0.0	0.0	0.0	0.006408572106049051	0.0
27	0.0	0.0	0.0	0.02755686005601092	0.0
28	0.0	0.0	0.0	0.1006145820649701	0.0
29	0.0	0.0	0.0	0.19225716318147154	0.0
30	0.0	0.0	0.0	0.31850603367063784	0.0
31	0.0	0.0	0.0	0.7010977884017662	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAG	40	5.907779E-5	27.75	1
GGTATCA	60	1.3250683E-6	24.666666	1
TTGCATG	40	0.001923755	23.125	25
CGGCCTT	65	2.6582493E-6	22.76923	24
TCGGCCT	75	3.69806E-7	22.2	23
GAATACA	45	0.0038112248	20.555555	1
TATGCCG	45	0.0038112248	20.555555	33
TCTCCGA	45	0.0038112248	20.555555	1
GCCGTCT	45	0.0038112248	20.555555	36
CCGGCGT	55	5.1157584E-4	20.181818	26
AACGTTG	50	0.0070083234	18.5	12
ATCTCGC	80	1.6021258E-5	18.5	11
ACGTTGC	50	0.0070083234	18.5	13
TGTATTC	50	0.0070083234	18.5	3
ACGGGTT	60	9.188127E-4	18.5	8
CCCACGA	50	0.0070083234	18.5	9
GGAACGT	50	0.0070083234	18.5	10
GAACGTT	50	0.0070083234	18.5	11
TCTTATA	190	0.0	17.526316	37
CTTATAC	95	3.5661415E-6	17.526316	37
>>END_MODULE