FastQCFastQC Report
Fri 10 Feb 2017
ERR1632245.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632245.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367448
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6610.17988939931636586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4820.13117502340467224No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4500.122466308157889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3050.031.5409851
ACTACTC502.6972572E-422.1999993
GCCGTCT603.7174097E-521.58333436
CTTATAC4550.020.73626337
ATTTATA555.1339454E-420.1818182
TGCATCA1457.2759576E-1219.1379314
TACCCTA701.2163342E-418.55
CTAACAC609.220517E-418.53
CGCGGGT500.007025999518.49999822
TACGCCG500.007025999518.49999810
TAACCCG500.007025999518.49999828
GCGGGTA500.007025999518.49999823
ACCCGTT500.007025999518.49999830
TATACTA500.007025999518.4999985
GTATCAA5350.017.981312
ATCAAGC1552.0008883E-1117.90322530
GCATCAG1900.017.52631615
CATCAGA1703.6379788E-1217.41176416
TAATACA752.0624472E-417.2666664
ATACTTT752.0624472E-417.2666666
Produced by FastQC (version 0.11.5)