##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632245.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	367448
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.431296401123426	33.0	31.0	34.0	31.0	34.0
2	32.62087696762535	34.0	31.0	34.0	31.0	34.0
3	32.703370272800505	34.0	31.0	34.0	31.0	34.0
4	36.2028668002003	37.0	35.0	37.0	35.0	37.0
5	36.027935925627574	37.0	35.0	37.0	35.0	37.0
6	36.04838507761642	37.0	35.0	37.0	35.0	37.0
7	36.02544033441467	37.0	35.0	37.0	35.0	37.0
8	36.01049128039886	37.0	35.0	37.0	35.0	37.0
9	37.76367540441096	39.0	37.0	39.0	35.0	39.0
10	37.68368041192223	39.0	37.0	39.0	35.0	39.0
11	37.777459667762514	39.0	37.0	39.0	35.0	39.0
12	37.72863371143672	39.0	37.0	39.0	35.0	39.0
13	37.754708149180296	39.0	37.0	39.0	35.0	39.0
14	39.067971522501146	40.0	38.0	41.0	36.0	41.0
15	39.060914741677735	40.0	38.0	41.0	36.0	41.0
16	39.058108902484165	40.0	38.0	41.0	36.0	41.0
17	39.03986414404215	40.0	38.0	41.0	36.0	41.0
18	39.027984912150835	40.0	38.0	41.0	36.0	41.0
19	39.063497420043106	40.0	39.0	41.0	36.0	41.0
20	39.03441575406588	40.0	39.0	41.0	36.0	41.0
21	39.019662646143125	40.0	39.0	41.0	36.0	41.0
22	38.97261109054887	40.0	38.0	41.0	36.0	41.0
23	38.926283446909494	40.0	38.0	41.0	35.0	41.0
24	38.8710157627746	40.0	38.0	41.0	35.0	41.0
25	38.805735777579415	40.0	38.0	41.0	35.0	41.0
26	38.712530752650714	40.0	38.0	41.0	35.0	41.0
27	38.618963771744575	40.0	38.0	41.0	35.0	41.0
28	38.54340750255818	40.0	38.0	41.0	35.0	41.0
29	38.4728043151684	40.0	38.0	41.0	34.0	41.0
30	38.409660686682194	40.0	38.0	41.0	34.0	41.0
31	38.32568689991509	40.0	38.0	41.0	34.0	41.0
32	38.22371328732229	40.0	38.0	41.0	34.0	41.0
33	38.1513220918334	40.0	38.0	41.0	34.0	41.0
34	38.07421730421719	40.0	38.0	41.0	33.0	41.0
35	38.03432050249287	40.0	38.0	41.0	33.0	41.0
36	38.01166967843069	40.0	37.0	41.0	33.0	41.0
37	37.954208486643005	40.0	37.0	41.0	33.0	41.0
38	37.918837495373495	40.0	37.0	41.0	33.0	41.0
39	37.87052045459494	40.0	37.0	41.0	33.0	41.0
40	37.79546493653524	40.0	37.0	41.0	33.0	41.0
41	37.747792884979646	40.0	37.0	41.0	33.0	41.0
42	37.701073349154164	40.0	37.0	41.0	33.0	41.0
43	36.82323757375193	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	1.0
16	1.0
17	6.0
18	7.0
19	16.0
20	33.0
21	60.0
22	110.0
23	226.0
24	307.0
25	493.0
26	793.0
27	1180.0
28	1636.0
29	2509.0
30	3294.0
31	4458.0
32	6112.0
33	8224.0
34	11669.0
35	17457.0
36	26888.0
37	48723.0
38	108981.0
39	124262.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.94784568156583	18.009350982996235	12.311401885436851	26.73140145000109
2	19.3823343711219	20.044196729877424	33.575907339269776	26.997561559730897
3	20.081480917027715	21.234297097820644	30.13052186976116	28.55370011539048
4	15.315636498225599	15.311554287953669	33.74763231804228	35.62517689577845
5	16.305708562844266	34.63973133613464	33.04549215127038	16.009067949750712
6	35.61918965404629	33.90983213951362	15.231815113975312	15.239163092464784
7	30.487851342230737	29.529620517733125	20.118492956826543	19.864035183209598
8	28.888441357688706	31.86410049857395	19.363828351222487	19.88362979251486
9	27.77835231107531	13.55838104983562	18.571607411116673	40.09165922797239
10	17.809866974374604	25.846378263046745	31.859473993599096	24.484280768979556
11	37.30623108575907	20.51610023731249	20.388463129476825	21.789205547451612
12	23.655047789074917	24.221114280115827	27.40387755546363	24.719960375345625
13	32.22197426574645	18.258910104286866	22.948281117328165	26.570834512638523
14	23.96284644357841	19.45526986131371	23.926106551131042	32.655777143976835
15	26.81141277133091	25.891554723389433	20.98065576625808	26.316376739021575
16	26.08722866909059	24.734656332324573	22.35690492260129	26.821210075983544
17	24.871274302758486	25.19512965099824	24.212677712220504	25.720918334022773
18	25.190775293374845	23.409843025407675	25.122194160806426	26.27718752041105
19	26.003951579543227	23.814798284383095	24.748535847249133	25.432714288824542
20	26.402919596786482	23.555714005791295	24.561570616794757	25.479795780627462
21	27.417212775685268	23.657769262589536	23.5069996298796	25.418018331845595
22	27.587577017700465	23.775881213124034	23.496113735821123	25.14042803335438
23	26.873734514815702	23.521423439507085	23.36357797565914	26.241264070018072
24	26.215682218980646	23.719546711371407	23.78649495983105	26.2782761098169
25	26.155265506956088	23.040539069473777	24.18736800853454	26.616827415035598
26	25.88665607106312	23.621029370142168	24.224652195684833	26.267662363109885
27	26.057564607781238	23.670015893405324	24.089394962008228	26.18302453680521
28	25.665944569027456	23.559251921360303	24.606202782434522	26.168600727177722
29	25.44115085671986	23.858886155319937	24.938766845921055	25.761196142039143
30	25.418018331845595	23.99142191548192	24.495166663038034	26.09539308963445
31	25.66676101108184	24.26030349872635	23.850449587424617	26.222485902767197
32	24.80323746489299	24.174304935664367	24.66417016829592	26.35828743114672
33	25.150769632709935	23.916037099126953	24.88460952298012	26.04858374518299
34	25.751670984737977	23.26560492913283	25.017417430493566	25.96530665563563
35	25.30997583331519	23.367115891228146	25.92557314232218	25.397335133134487
36	25.07184690078596	23.33990115608195	24.954823539657312	26.63342840347478
37	24.588785351940956	22.566186235875552	26.01674250506194	26.82828590712155
38	24.364263786984825	22.464403126428774	26.84706407437243	26.324269012213975
39	23.81670331584333	22.106257211904815	27.759846291175894	26.31719318107596
40	23.559524068711763	21.482223335002505	28.8190437830659	26.139208813219827
41	22.87888354270536	21.482223335002505	29.30727613158869	26.33161699070345
42	22.28070366419194	21.610132590189632	29.436273976181663	26.672889769436765
43	21.598974548779694	20.67013563823997	30.265234808734842	27.465655004245498
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15.0
1	14.0
2	13.0
3	39.5
4	66.0
5	66.0
6	94.0
7	122.0
8	123.0
9	124.0
10	167.0
11	210.0
12	210.0
13	368.0
14	526.0
15	893.5
16	1261.0
17	1240.5
18	1220.0
19	1220.0
20	1485.0
21	1750.0
22	1903.0
23	2056.0
24	2613.5
25	3171.0
26	3171.0
27	3696.5
28	4222.0
29	5137.0
30	6052.0
31	7200.5
32	8349.0
33	8349.0
34	9685.0
35	11021.0
36	12642.5
37	14264.0
38	15304.5
39	16345.0
40	16345.0
41	17678.0
42	19011.0
43	19963.0
44	20915.0
45	21740.0
46	22565.0
47	22565.0
48	23194.0
49	23823.0
50	24773.5
51	25724.0
52	26888.0
53	28052.0
54	28052.0
55	27931.0
56	27810.0
57	27005.5
58	26201.0
59	24805.5
60	23410.0
61	23410.0
62	22326.5
63	21243.0
64	18380.0
65	15517.0
66	13672.0
67	11827.0
68	11827.0
69	10248.5
70	8670.0
71	7799.0
72	6928.0
73	6529.5
74	6131.0
75	6131.0
76	5450.5
77	4770.0
78	3387.5
79	2005.0
80	1498.0
81	991.0
82	991.0
83	769.5
84	548.0
85	407.0
86	266.0
87	209.5
88	153.0
89	153.0
90	113.5
91	74.0
92	45.5
93	17.0
94	11.5
95	6.0
96	6.0
97	5.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	367448.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.34833844590247
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.59779091233148	69.01741350559362
2	4.795177378821165	7.226172960951054
3	1.2230248101989145	2.764586619798104
4	0.5720279990847016	1.7240543710226592
5	0.3830975159967642	1.4432880646554367
6	0.254780170146998	1.1518357493724376
7	0.17809100286494498	0.9393202810626644
8	0.14398735418995656	0.8679366316347905
9	0.11311426069375208	0.7670674438029788
>10	0.676085159454615	9.511861002898652
>50	0.04513177889342912	2.294455902385929
>100	0.017330603092288108	2.1121834982081387
>500	3.610542310893355E-4	0.17982396861354707
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	661	0.17988939931636586	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	482	0.13117502340467224	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	450	0.122466308157889	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.442947029239511E-4	0.0
2	0.0	0.0	0.0	5.442947029239511E-4	0.0
3	0.0	0.0	0.0	8.164420543859268E-4	0.0
4	0.0	0.0	0.0	8.164420543859268E-4	0.0
5	0.0	0.0	0.0	8.164420543859268E-4	0.0
6	0.0	0.0	0.0	8.164420543859268E-4	0.0
7	0.0	0.0	0.0	8.164420543859268E-4	0.0
8	0.0	0.0	0.0	8.164420543859268E-4	0.0
9	0.0	0.0	0.0	0.0010885894058479022	0.0
10	0.0	0.0	0.0	0.0013607367573098778	0.0
11	0.0	2.7214735146197555E-4	0.0	0.0013607367573098778	0.0
12	0.0	2.7214735146197555E-4	0.0	0.0013607367573098778	0.0
13	0.0	2.7214735146197555E-4	0.0	0.0013607367573098778	0.0
14	0.0	2.7214735146197555E-4	0.0	0.0013607367573098778	0.0
15	2.7214735146197555E-4	2.7214735146197555E-4	0.0	0.0013607367573098778	0.0
16	2.7214735146197555E-4	2.7214735146197555E-4	0.0	0.0013607367573098778	0.0
17	2.7214735146197555E-4	2.7214735146197555E-4	0.0	0.0013607367573098778	0.0
18	2.7214735146197555E-4	2.7214735146197555E-4	0.0	0.0013607367573098778	0.0
19	5.442947029239511E-4	2.7214735146197555E-4	0.0	0.001905031460233829	0.0
20	5.442947029239511E-4	2.7214735146197555E-4	0.0	0.0021771788116958044	0.0
21	5.442947029239511E-4	2.7214735146197555E-4	0.0	0.0021771788116958044	0.0
22	8.164420543859268E-4	2.7214735146197555E-4	0.0	0.0029936208660817313	0.0
23	8.164420543859268E-4	2.7214735146197555E-4	0.0	0.0040822102719296335	0.0
24	8.164420543859268E-4	2.7214735146197555E-4	0.0	0.00789227319239729	0.0
25	8.164420543859268E-4	2.7214735146197555E-4	0.0	0.010613746707017048	0.0
26	8.164420543859268E-4	2.7214735146197555E-4	0.0	0.013607367573098779	0.0
27	8.164420543859268E-4	2.7214735146197555E-4	0.0	0.04027780801637239	0.0
28	8.164420543859268E-4	2.7214735146197555E-4	0.0	0.12164986610350308	0.0
29	8.164420543859268E-4	2.7214735146197555E-4	0.0	0.2585399838888768	0.0
30	8.164420543859268E-4	2.7214735146197555E-4	0.0	0.4446887722888681	0.0
31	8.164420543859268E-4	2.7214735146197555E-4	0.0	0.9478892251420609	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	305	0.0	31.540985	1
ACTACTC	50	2.6972572E-4	22.199999	3
GCCGTCT	60	3.7174097E-5	21.583334	36
CTTATAC	455	0.0	20.736263	37
ATTTATA	55	5.1339454E-4	20.181818	2
TGCATCA	145	7.2759576E-12	19.13793	14
TACCCTA	70	1.2163342E-4	18.5	5
CTAACAC	60	9.220517E-4	18.5	3
CGCGGGT	50	0.0070259995	18.499998	22
TACGCCG	50	0.0070259995	18.499998	10
TAACCCG	50	0.0070259995	18.499998	28
GCGGGTA	50	0.0070259995	18.499998	23
ACCCGTT	50	0.0070259995	18.499998	30
TATACTA	50	0.0070259995	18.499998	5
GTATCAA	535	0.0	17.98131	2
ATCAAGC	155	2.0008883E-11	17.903225	30
GCATCAG	190	0.0	17.526316	15
CATCAGA	170	3.6379788E-12	17.411764	16
TAATACA	75	2.0624472E-4	17.266666	4
ATACTTT	75	2.0624472E-4	17.266666	6
>>END_MODULE