FastQCFastQC Report
Fri 10 Feb 2017
ERR1632244.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632244.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences876424
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3200.024.8593751
TCGTACC400.001930823323.1254
TATCGGT1450.021.68965515
GCGCTAT1600.020.812511
TTAACGG450.003825094320.55555535
GTATAAC450.003825094320.5555551
TGCGCTA1700.019.58823610
CTATCGG1750.019.02857214
GCTATCG1750.019.02857213
TTAGGCA902.1510295E-618.54
TCGTTTA701.21877805E-418.530
ACCGATA1103.848436E-818.519
CGCTATC1800.018.512
CTAAACA701.21877805E-418.54
GCTTAGA1002.8729846E-718.51
GTCTTAA609.234429E-418.51
GTATCAA6500.017.9307691
GTCACGC953.6044094E-617.52631613
ATCGGTC1800.017.47222116
AATACCG1705.456968E-1217.4117645
Produced by FastQC (version 0.11.5)