##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632244.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	876424
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.500581910125696	34.0	31.0	34.0	31.0	34.0
2	32.6761601690506	34.0	31.0	34.0	31.0	34.0
3	32.770082745337874	34.0	31.0	34.0	31.0	34.0
4	36.24613771416575	37.0	37.0	37.0	35.0	37.0
5	36.067256259527355	37.0	35.0	37.0	35.0	37.0
6	36.08483450932425	37.0	35.0	37.0	35.0	37.0
7	36.057549770430754	37.0	35.0	37.0	35.0	37.0
8	36.035974596770515	37.0	35.0	37.0	35.0	37.0
9	37.801214937062426	39.0	38.0	39.0	35.0	39.0
10	37.72055078363897	39.0	37.0	39.0	35.0	39.0
11	37.81373741476728	39.0	37.0	39.0	35.0	39.0
12	37.749767235949726	39.0	37.0	39.0	35.0	39.0
13	37.78207808092886	39.0	37.0	39.0	35.0	39.0
14	39.123169835604685	40.0	38.0	41.0	36.0	41.0
15	39.115002555840555	40.0	38.0	41.0	36.0	41.0
16	39.109653546685166	40.0	38.0	41.0	36.0	41.0
17	39.08981953940102	40.0	38.0	41.0	36.0	41.0
18	39.07613780544577	40.0	38.0	41.0	36.0	41.0
19	39.09953173349886	40.0	39.0	41.0	36.0	41.0
20	39.072901928746816	40.0	39.0	41.0	36.0	41.0
21	39.046911084132795	40.0	39.0	41.0	36.0	41.0
22	39.01236844267159	40.0	39.0	41.0	36.0	41.0
23	38.955232855330294	40.0	38.0	41.0	36.0	41.0
24	38.9014643597163	40.0	38.0	41.0	35.0	41.0
25	38.84621826878315	40.0	38.0	41.0	35.0	41.0
26	38.744582530829824	40.0	38.0	41.0	35.0	41.0
27	38.66726607212947	40.0	38.0	41.0	35.0	41.0
28	38.59985920056959	40.0	38.0	41.0	35.0	41.0
29	38.52093393152173	40.0	38.0	41.0	34.0	41.0
30	38.444337443976885	40.0	38.0	41.0	34.0	41.0
31	38.37772813158928	40.0	38.0	41.0	34.0	41.0
32	38.280923388679454	40.0	38.0	41.0	34.0	41.0
33	38.19936355006253	40.0	38.0	41.0	34.0	41.0
34	38.122287842414174	40.0	38.0	41.0	34.0	41.0
35	38.08997243343405	40.0	38.0	41.0	33.0	41.0
36	38.048104570390585	40.0	38.0	41.0	33.0	41.0
37	37.98025384973483	40.0	37.0	41.0	33.0	41.0
38	37.929956276870556	40.0	37.0	41.0	33.0	41.0
39	37.873633081704746	40.0	37.0	41.0	33.0	41.0
40	37.793165180323676	40.0	37.0	41.0	33.0	41.0
41	37.74356133560925	40.0	37.0	41.0	33.0	41.0
42	37.67429463364764	40.0	37.0	41.0	33.0	41.0
43	36.76962976824003	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	1.0
13	0.0
14	0.0
15	1.0
16	1.0
17	7.0
18	15.0
19	34.0
20	73.0
21	151.0
22	279.0
23	462.0
24	747.0
25	1208.0
26	1872.0
27	2788.0
28	4000.0
29	5611.0
30	7702.0
31	10426.0
32	14178.0
33	18883.0
34	26885.0
35	40053.0
36	63314.0
37	114319.0
38	260014.0
39	303398.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.16994514070815	16.74315171652077	11.235885826951339	27.851017315819742
2	19.677918450430383	18.995600303049663	32.409313300411675	28.917167946108275
3	20.370163299955273	20.179844458846404	28.547483866256517	30.90250837494181
4	15.417309430138836	14.400107710423265	32.638197949850756	37.544384909587144
5	17.02748897793762	33.756264091353046	32.57042253521127	16.645824395498067
6	37.634181628983235	32.95003331720719	14.417108614095461	14.998676439714112
7	32.70745666481064	28.260065904174237	19.13639973346234	19.896077697552784
8	30.065242394092355	31.279723056420178	18.494929394904748	20.160105154582713
9	28.08617746661433	12.922854691336614	17.39717305778938	41.59379478425967
10	18.039099796445555	24.859656969685908	31.202135039661165	25.899108194207372
11	39.96798353308444	19.147467435852967	19.49969421193395	21.38485481912864
12	24.227999233247836	22.829817531240586	27.128992359862348	25.813190875649227
13	33.148795560139845	17.137139101622047	22.268331309959564	27.44573402827855
14	24.50674559345705	17.697370222631967	22.74983341396402	35.046050769946966
15	27.496964939344426	24.65701532591531	20.09187333984464	27.754146394895624
16	27.814277107883857	23.525371281480197	21.4235347274835	27.23681688315245
17	26.698949366973064	23.44276286363678	22.902613346964483	26.955674422425673
18	27.095218752567252	22.631739888455815	23.599878597573777	26.67316276140316
19	27.89209332469216	22.875685741148118	22.885498343267642	26.346722590892078
20	27.72642008890674	22.512163062627224	22.84841583525782	26.91300101320822
21	28.229487097569212	22.315682820187487	22.647371591832265	26.807458490411033
22	28.47126504979325	22.215160698474712	22.36394713061258	26.949627121119455
23	27.554927751864394	22.385055635172016	22.782123720938724	27.277892892024862
24	27.315888200231853	22.66015079459257	22.495732659078254	27.528228346097322
25	27.57934515713855	22.044010661506302	22.750175714037955	27.62646846731719
26	27.306417898186265	22.51296176279974	22.749377013865434	27.431243325148557
27	27.29934369665824	22.414949841629166	22.70179730358822	27.583909158124374
28	27.177142570262795	22.312145719423476	23.104228090513267	27.406483619800458
29	26.82811059487189	22.849556835504277	23.07558898432722	27.246743585296613
30	26.734776774711783	23.14541819941033	22.847503035060658	27.272301990817233
31	27.415155221673526	22.73420171058757	22.554608271795388	27.296034795943513
32	26.60299124624611	22.85343623634223	23.039875676613146	27.50369684079852
33	26.585191642401394	22.675782497969017	23.456683066643542	27.282342792986043
34	27.195740874280027	22.057132164340548	23.354107144487145	27.393019816892277
35	26.591695343806194	22.617477385375114	23.78072713663706	27.01010013418163
36	26.613716648562797	22.805400125966425	23.486120873002108	27.094762352468667
37	26.55107573503236	22.08463027028014	23.828192746889634	27.536101247797866
38	26.134268345001963	22.323783921937327	24.48438198862651	27.057565744434203
39	26.112931640393235	21.757277299571896	24.63259792064115	27.497193139393715
40	25.860542385877157	21.699428587076575	25.296660064078573	27.143368962967696
41	25.209715845298625	21.533983551340448	26.104944638668044	27.151355964692886
42	25.17731143829927	21.650821976577546	26.20010405922248	26.971762525900704
43	24.538009000209943	20.84230920193879	26.841916697854007	27.77776509999726
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9.0
1	9.0
2	9.0
3	36.5
4	64.0
5	64.0
6	88.0
7	112.0
8	110.0
9	108.0
10	163.0
11	218.0
12	218.0
13	373.0
14	528.0
15	911.5
16	1295.0
17	1300.0
18	1305.0
19	1305.0
20	1599.5
21	1894.0
22	2073.0
23	2252.0
24	2915.5
25	3579.0
26	3579.0
27	4533.0
28	5487.0
29	6902.0
30	8317.0
31	10122.5
32	11928.0
33	11928.0
34	14588.0
35	17248.0
36	20372.5
37	23497.0
38	27041.5
39	30586.0
40	30586.0
41	33978.5
42	37371.0
43	40721.5
44	44072.0
45	47258.5
46	50445.0
47	50445.0
48	53846.5
49	57248.0
50	60964.5
51	64681.0
52	67595.5
53	70510.0
54	70510.0
55	71234.5
56	71959.0
57	71622.5
58	71286.0
59	70288.5
60	69291.0
61	69291.0
62	65495.5
63	61700.0
64	55884.5
65	50069.0
66	44689.0
67	39309.0
68	39309.0
69	33867.5
70	28426.0
71	24357.0
72	20288.0
73	16572.0
74	12856.0
75	12856.0
76	10217.0
77	7578.0
78	6234.0
79	4890.0
80	3908.5
81	2927.0
82	2927.0
83	2202.5
84	1478.0
85	1181.5
86	885.0
87	669.5
88	454.0
89	454.0
90	328.0
91	202.0
92	119.0
93	36.0
94	28.5
95	21.0
96	21.0
97	13.0
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	876424.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.45320760428883
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.78171499014218	57.004701148918535
2	7.831972456134069	10.409193831570983
3	2.387198055481937	4.759109039204873
4	1.099464891868489	2.9225187485185473
5	0.6753493829747773	2.2439566376125617
6	0.44002018110907887	1.7544451467191031
7	0.3117535407058542	1.4501915933328768
8	0.2248389603316732	1.1953016086762513
9	0.168722020590621	1.009090751555127
>10	0.9980388038795144	12.11355721025011
>50	0.05773377582683383	2.5772979319229385
>100	0.02267754228947495	2.36541195293978
>500	5.153986655762593E-4	0.1952243987783218
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.1410002464560532E-4	0.0	0.0	0.0
6	0.0	1.1410002464560532E-4	0.0	0.0	0.0
7	0.0	1.1410002464560532E-4	0.0	0.0	0.0
8	0.0	1.1410002464560532E-4	0.0	0.0	0.0
9	0.0	1.1410002464560532E-4	0.0	1.1410002464560532E-4	0.0
10	0.0	1.1410002464560532E-4	0.0	2.2820004929121064E-4	1.1410002464560532E-4
11	0.0	2.2820004929121064E-4	0.0	2.2820004929121064E-4	1.1410002464560532E-4
12	0.0	2.2820004929121064E-4	0.0	2.2820004929121064E-4	1.1410002464560532E-4
13	0.0	2.2820004929121064E-4	0.0	2.2820004929121064E-4	1.1410002464560532E-4
14	0.0	2.2820004929121064E-4	0.0	2.2820004929121064E-4	1.1410002464560532E-4
15	0.0	2.2820004929121064E-4	0.0	2.2820004929121064E-4	1.1410002464560532E-4
16	0.0	2.2820004929121064E-4	0.0	3.4230007393681595E-4	1.1410002464560532E-4
17	0.0	2.2820004929121064E-4	0.0	5.705001232280266E-4	1.1410002464560532E-4
18	0.0	2.2820004929121064E-4	0.0	6.846001478736319E-4	1.1410002464560532E-4
19	0.0	2.2820004929121064E-4	0.0	6.846001478736319E-4	1.1410002464560532E-4
20	0.0	2.2820004929121064E-4	0.0	7.987001725192372E-4	1.1410002464560532E-4
21	0.0	2.2820004929121064E-4	0.0	0.001026900221810448	1.1410002464560532E-4
22	0.0	2.2820004929121064E-4	0.0	0.0013692002957472638	1.1410002464560532E-4
23	0.0	3.4230007393681595E-4	0.0	0.0018256003943296851	1.1410002464560532E-4
24	0.0	3.4230007393681595E-4	0.0	0.0022820004929121063	1.1410002464560532E-4
25	0.0	3.4230007393681595E-4	0.0	0.0030807006654313437	1.1410002464560532E-4
26	0.0	3.4230007393681595E-4	0.0	0.004449900961178608	1.1410002464560532E-4
27	0.0	3.4230007393681595E-4	0.0	0.015974003450384744	1.1410002464560532E-4
28	0.0	5.705001232280266E-4	0.0	0.05716411234744827	1.1410002464560532E-4
29	0.0	7.987001725192372E-4	0.0	0.11672432521245425	1.1410002464560532E-4
30	0.0	7.987001725192372E-4	0.0	0.1929431416757186	1.1410002464560532E-4
31	0.0	7.987001725192372E-4	0.0	0.4101895886009511	1.1410002464560532E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	320	0.0	24.859375	1
TCGTACC	40	0.0019308233	23.125	4
TATCGGT	145	0.0	21.689655	15
GCGCTAT	160	0.0	20.8125	11
TTAACGG	45	0.0038250943	20.555555	35
GTATAAC	45	0.0038250943	20.555555	1
TGCGCTA	170	0.0	19.588236	10
CTATCGG	175	0.0	19.028572	14
GCTATCG	175	0.0	19.028572	13
TTAGGCA	90	2.1510295E-6	18.5	4
TCGTTTA	70	1.21877805E-4	18.5	30
ACCGATA	110	3.848436E-8	18.5	19
CGCTATC	180	0.0	18.5	12
CTAAACA	70	1.21877805E-4	18.5	4
GCTTAGA	100	2.8729846E-7	18.5	1
GTCTTAA	60	9.234429E-4	18.5	1
GTATCAA	650	0.0	17.930769	1
GTCACGC	95	3.6044094E-6	17.526316	13
ATCGGTC	180	0.0	17.472221	16
AATACCG	170	5.456968E-12	17.411764	5
>>END_MODULE