FastQCFastQC Report
Fri 10 Feb 2017
ERR1632243.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632243.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720868
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA10700.14843216788649238No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10380.14399307501512065No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC10360.14371563171065993No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8380.11624874456904731No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG7870.10917394030529862No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAACC358.866174E-426.428573
GGTATCA3450.024.6666661
CTAGGTA400.001930492823.1254
ATCTCGC3650.019.76712411
AAATCTC3850.019.220789
GGATAGA609.2322595E-418.51
CGCGTAA701.2183972E-418.510
GCGTAAC701.2183972E-418.511
TGCGTTA609.2322595E-418.537
TCTCGCC4000.018.03749812
CGGCCTT3950.017.79746824
TTCCTCG4100.017.5975619
CTTATAC6250.017.46437
CGTTTAT852.7208356E-517.41176431
TCGCGTA752.0659191E-417.2666669
GTTCTAA650.001579161717.0769231
ATGCGTT650.001579161717.07692336
GTATCAA6650.016.9699251
GTATTAA1901.8189894E-1216.5526311
TCGTTTA904.442259E-516.44444530
Produced by FastQC (version 0.11.5)