##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632243.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	720868
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.241012224151994	33.0	31.0	34.0	30.0	34.0
2	32.4303270501673	34.0	31.0	34.0	31.0	34.0
3	32.511021435269704	34.0	31.0	34.0	31.0	34.0
4	36.08959060465994	37.0	35.0	37.0	35.0	37.0
5	35.82509141756882	37.0	35.0	37.0	35.0	37.0
6	35.832983292364204	37.0	35.0	37.0	35.0	37.0
7	35.81366075342504	37.0	35.0	37.0	35.0	37.0
8	35.78372462087373	37.0	35.0	37.0	35.0	37.0
9	37.50688475560019	39.0	37.0	39.0	35.0	39.0
10	37.39870406232487	39.0	37.0	39.0	35.0	39.0
11	37.496648484882115	39.0	37.0	39.0	35.0	39.0
12	37.44968010786996	39.0	37.0	39.0	35.0	39.0
13	37.483360337814965	39.0	37.0	39.0	35.0	39.0
14	38.73408446483961	40.0	38.0	41.0	35.0	41.0
15	38.72154680191103	40.0	38.0	41.0	35.0	41.0
16	38.705127707153046	40.0	38.0	41.0	35.0	41.0
17	38.6939855840459	40.0	38.0	41.0	35.0	41.0
18	38.68757525649634	40.0	38.0	41.0	35.0	41.0
19	38.722333353679176	40.0	38.0	41.0	35.0	41.0
20	38.70925606352342	40.0	38.0	41.0	35.0	41.0
21	38.666907117530535	40.0	38.0	41.0	35.0	41.0
22	38.63923630956014	40.0	38.0	41.0	35.0	41.0
23	38.580586182213665	40.0	38.0	41.0	35.0	41.0
24	38.52375608294445	40.0	38.0	41.0	34.0	41.0
25	38.4534644345428	40.0	38.0	41.0	34.0	41.0
26	38.35513991465844	40.0	38.0	41.0	34.0	41.0
27	38.27126602928691	40.0	38.0	41.0	34.0	41.0
28	38.19937075858548	40.0	38.0	41.0	34.0	41.0
29	38.129291354311746	40.0	38.0	41.0	34.0	41.0
30	38.06619658522781	40.0	38.0	41.0	34.0	41.0
31	37.972241797388705	40.0	37.0	41.0	33.0	41.0
32	37.87131902095807	40.0	37.0	41.0	33.0	41.0
33	37.795148348934894	40.0	37.0	41.0	33.0	41.0
34	37.716372484282836	40.0	37.0	41.0	33.0	41.0
35	37.69539499603256	40.0	37.0	41.0	33.0	41.0
36	37.663615252723105	40.0	37.0	41.0	33.0	41.0
37	37.61676340189882	40.0	37.0	41.0	33.0	41.0
38	37.55330656930256	39.0	37.0	41.0	33.0	41.0
39	37.50044945815323	39.0	37.0	41.0	33.0	41.0
40	37.451360027078465	39.0	37.0	41.0	32.0	41.0
41	37.412072667950305	39.0	36.0	41.0	32.0	41.0
42	37.37084736734048	39.0	36.0	41.0	32.0	41.0
43	36.482546041716375	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	0.0
16	3.0
17	4.0
18	29.0
19	44.0
20	85.0
21	135.0
22	286.0
23	527.0
24	799.0
25	1232.0
26	1825.0
27	2742.0
28	3990.0
29	5670.0
30	7749.0
31	10673.0
32	14242.0
33	19118.0
34	27110.0
35	40011.0
36	64418.0
37	115170.0
38	226567.0
39	178437.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.40873502499764	18.555824367290544	12.817880666085884	26.21755994162593
2	19.130964337437646	20.916312001642464	33.831020380985144	26.121703279934742
3	19.951919075336953	21.818141462792077	30.763468485215046	27.46647097665592
4	15.609653917222015	15.747681961191232	33.482690312234695	35.15997380935206
5	15.737139115621723	35.378321689962654	33.376706969930694	15.507832224484927
6	35.15387005665392	34.054362241076035	15.589539277648612	15.202228424621428
7	29.544382605414583	30.21177247429488	20.49057525094747	19.75326966934307
8	27.668865867260024	32.900752981128306	19.863692104518442	19.566689047093227
9	26.773140158808545	14.426358223696987	19.111404584473163	39.689097033021305
10	17.202317206478856	26.911168202777763	32.54659660298418	23.3399179877592
11	36.67203981866305	20.89550375380791	21.351481824689124	21.080974602839913
12	22.561550797094615	24.873485853165906	27.85211716985634	24.71284617988314
13	31.22707624696893	18.858515012457204	24.002175155506972	25.91223358506689
14	23.44950809302119	20.054573097987426	24.019792805340227	32.47612600365115
15	26.15041866194643	26.951397481924566	21.575794736345628	25.322389119783374
16	25.947746328037862	25.38495258493927	22.93845752620452	25.728843560818348
17	24.904282059961048	25.785441994928338	24.35300221399757	24.957273731113048
18	24.435125432117946	24.491723866227936	25.48871638080758	25.584434320846533
19	25.75090030352298	24.747804036245196	25.440025080874722	24.061270579357107
20	25.9305448431613	24.044346537785003	25.29963876881759	24.725469850236102
21	25.812076552156565	25.03634507288436	23.963887979491393	25.187690395467687
22	26.20937536414434	24.43290588568226	24.149220106871162	25.208498643302242
23	25.493294195331185	24.639739869157737	24.329142089813946	25.537823845697133
24	26.372650748819478	24.44122918481608	24.15976295244067	25.02635711392377
25	25.875749790530307	23.742626944183957	24.60797261079698	25.773650654488755
26	25.50730508220645	24.203182829588773	24.53167570207028	25.757836386134493
27	25.33681617161533	24.297652274757652	24.70147100440025	25.664060549226765
28	24.853509935244734	24.5838350433089	25.10945138360976	25.45320363783661
29	25.04231010393026	25.01040412391728	25.009710515656124	24.937575256496338
30	24.633774838111833	25.545592258222033	24.60755644584029	25.213076457825846
31	25.34083909953001	24.80481863531187	24.707574757098385	25.146767508059725
32	24.525294506067684	24.706326262228316	25.016646598267645	25.751732633436358
33	24.68565673604599	24.236753469428525	25.300748542035436	25.77684125249005
34	25.07005443437634	24.021734908471455	25.284379387072253	25.62383127007996
35	24.879728327516272	23.941276350177844	25.652824095396102	25.52617122690978
36	24.426385968027432	24.02131874351476	25.819151356420317	25.73314393203749
37	25.037732289406662	22.623420653989356	26.05192628886287	26.286920767741112
38	23.78479832646199	23.06996565251891	27.34994478878241	25.795291232236693
39	23.66078116936804	22.619952612683598	27.950193377983208	25.76907283996515
40	23.334091678365525	22.36068184466504	28.77156428083921	25.53366219613022
41	22.576255292231032	22.534361353257463	29.37777790108591	25.511605453425588
42	22.86216061747782	21.908865423350736	29.128356370375712	26.10061758879573
43	21.536397787112204	21.8310425764495	29.881060055377684	26.75149958106061
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27.0
1	26.0
2	25.0
3	70.5
4	116.0
5	116.0
6	165.0
7	214.0
8	213.5
9	213.0
10	325.0
11	437.0
12	437.0
13	746.5
14	1056.0
15	1784.0
16	2512.0
17	2519.5
18	2527.0
19	2527.0
20	3113.0
21	3699.0
22	4320.5
23	4942.0
24	5938.5
25	6935.0
26	6935.0
27	7851.5
28	8768.0
29	11634.0
30	14500.0
31	15858.5
32	17217.0
33	17217.0
34	19611.0
35	22005.0
36	24361.5
37	26718.0
38	31073.0
39	35428.0
40	35428.0
41	39330.0
42	43232.0
43	42704.5
44	42177.0
45	44917.5
46	47658.0
47	47658.0
48	49661.0
49	51664.0
50	54521.0
51	57378.0
52	60155.5
53	62933.0
54	62933.0
55	57102.5
56	51272.0
57	49036.0
58	46800.0
59	43304.5
60	39809.0
61	39809.0
62	37095.0
63	34381.0
64	31346.5
65	28312.0
66	24910.5
67	21509.0
68	21509.0
69	18657.0
70	15805.0
71	13773.5
72	11742.0
73	9557.5
74	7373.0
75	7373.0
76	5938.5
77	4504.0
78	3846.5
79	3189.0
80	2498.5
81	1808.0
82	1808.0
83	1407.0
84	1006.0
85	776.5
86	547.0
87	411.5
88	276.0
89	276.0
90	192.5
91	109.0
92	69.0
93	29.0
94	20.5
95	12.0
96	12.0
97	7.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	720868.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.58480462761722
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.19450806220401	58.49804385116079
2	6.490302380310586	8.513304271736576
3	1.6208501772698958	3.189094266206545
4	0.7297769366595147	1.9144911125022104
5	0.41056631635725693	1.346345582248559
6	0.2652983405468876	1.043972389967921
7	0.17755184189346276	0.8151292003299435
8	0.13936726606479918	0.7312299933076002
9	0.10168017817579185	0.6001807158144554
>10	0.7044166555764017	9.085466897682332
>50	0.09373850284960747	4.35620428791088
>100	0.06855777305255137	8.380693359543947
>500	0.002750774844439609	1.089531769535203
>1k	6.34794194870679E-4	0.43631230205307325
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1070	0.14843216788649238	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1038	0.14399307501512065	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	1036	0.14371563171065993	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	838	0.11624874456904731	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	787	0.10917394030529862	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	4.1616495669110016E-4	0.0
11	0.0	1.3872165223036674E-4	0.0	4.1616495669110016E-4	0.0
12	0.0	1.3872165223036674E-4	0.0	4.1616495669110016E-4	0.0
13	0.0	1.3872165223036674E-4	0.0	4.1616495669110016E-4	0.0
14	0.0	1.3872165223036674E-4	0.0	4.1616495669110016E-4	0.0
15	0.0	1.3872165223036674E-4	0.0	4.1616495669110016E-4	0.0
16	0.0	1.3872165223036674E-4	0.0	4.1616495669110016E-4	0.0
17	0.0	1.3872165223036674E-4	0.0	5.54886608921467E-4	0.0
18	0.0	2.774433044607335E-4	0.0	5.54886608921467E-4	0.0
19	0.0	2.774433044607335E-4	0.0	5.54886608921467E-4	0.0
20	0.0	2.774433044607335E-4	0.0	6.936082611518336E-4	0.0
21	0.0	2.774433044607335E-4	0.0	6.936082611518336E-4	0.0
22	0.0	2.774433044607335E-4	0.0	0.0012484948700733005	0.0
23	0.0	4.1616495669110016E-4	0.0	0.0016646598267644006	0.0
24	0.0	4.1616495669110016E-4	0.0	0.0033293196535288012	0.0
25	0.0	4.1616495669110016E-4	0.0	0.004577814523602102	0.0
26	0.0	4.1616495669110016E-4	0.0	0.00721352591597907	0.0
27	0.0	4.1616495669110016E-4	0.0	0.02885410366391628	0.0
28	0.0	4.1616495669110016E-4	0.0	0.10376379586831432	0.0
29	0.0	4.1616495669110016E-4	0.0	0.22084487035074382	0.0
30	0.0	4.1616495669110016E-4	0.0	0.3764905641532153	0.0
31	0.0	4.1616495669110016E-4	0.0	0.8255325524229123	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAACC	35	8.866174E-4	26.42857	3
GGTATCA	345	0.0	24.666666	1
CTAGGTA	40	0.0019304928	23.125	4
ATCTCGC	365	0.0	19.767124	11
AAATCTC	385	0.0	19.22078	9
GGATAGA	60	9.2322595E-4	18.5	1
CGCGTAA	70	1.2183972E-4	18.5	10
GCGTAAC	70	1.2183972E-4	18.5	11
TGCGTTA	60	9.2322595E-4	18.5	37
TCTCGCC	400	0.0	18.037498	12
CGGCCTT	395	0.0	17.797468	24
TTCCTCG	410	0.0	17.59756	19
CTTATAC	625	0.0	17.464	37
CGTTTAT	85	2.7208356E-5	17.411764	31
TCGCGTA	75	2.0659191E-4	17.266666	9
GTTCTAA	65	0.0015791617	17.076923	1
ATGCGTT	65	0.0015791617	17.076923	36
GTATCAA	665	0.0	16.969925	1
GTATTAA	190	1.8189894E-12	16.552631	1
TCGTTTA	90	4.442259E-5	16.444445	30
>>END_MODULE