FastQCFastQC Report
Fri 10 Feb 2017
ERR1632242.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632242.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009369
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17380.1721867820390759No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16000.1585148741441435No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12600.12483046338851302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6600.026.6287881
GCACCGT806.95838E-720.81256
TGCGAAA450.003825490620.55555510
CTTATAC10300.018.85922237
GTGAGTA700.002592215.8571439
TCTTATA17100.015.57894737
GTATCAA11400.015.4166662
GTGTTAC908.272785E-414.3888881
TATACTG908.272785E-414.3888885
GTAGTAC908.272785E-414.3888883
GTATTAA1956.712071E-1014.230771
TAAGGTG1203.299574E-513.8750015
GTGTTAG1601.7822458E-713.8751
GTGTAAT800.006297920413.8751
GGGCGTT950.001244889613.63157913
CTATACT1501.3049284E-613.5666664
AGACTAT1102.4558947E-413.4545456
CTCTTAT26350.013.1992437
TACAAGA1551.8866631E-613.1290322
CTAGATA850.0094036913.0588244
Produced by FastQC (version 0.11.5)