##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632242.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1009369
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.38116684780294	33.0	31.0	34.0	31.0	34.0
2	32.56708597153271	34.0	31.0	34.0	31.0	34.0
3	32.65355880753223	34.0	31.0	34.0	31.0	34.0
4	36.16379143801721	37.0	35.0	37.0	35.0	37.0
5	35.958731643234536	37.0	35.0	37.0	35.0	37.0
6	35.97623663892987	37.0	35.0	37.0	35.0	37.0
7	35.95111203137802	37.0	35.0	37.0	35.0	37.0
8	35.93632853792815	37.0	35.0	37.0	35.0	37.0
9	37.66460531282415	39.0	37.0	39.0	35.0	39.0
10	37.57620354894989	39.0	37.0	39.0	35.0	39.0
11	37.667689417844215	39.0	37.0	39.0	35.0	39.0
12	37.6155647736358	39.0	37.0	39.0	35.0	39.0
13	37.65245118484915	39.0	37.0	39.0	35.0	39.0
14	38.94196473242194	40.0	38.0	41.0	36.0	41.0
15	38.94141587467021	40.0	38.0	41.0	36.0	41.0
16	38.92438741431528	40.0	38.0	41.0	36.0	41.0
17	38.910068567590244	40.0	38.0	41.0	36.0	41.0
18	38.893241222981885	40.0	38.0	41.0	36.0	41.0
19	38.92479856227009	40.0	38.0	41.0	36.0	41.0
20	38.89590328214954	40.0	38.0	41.0	36.0	41.0
21	38.86658991904844	40.0	38.0	41.0	35.0	41.0
22	38.82606361003756	40.0	38.0	41.0	35.0	41.0
23	38.77220719082912	40.0	38.0	41.0	35.0	41.0
24	38.71847956495593	40.0	38.0	41.0	35.0	41.0
25	38.6539521225637	40.0	38.0	41.0	35.0	41.0
26	38.550864946317944	40.0	38.0	41.0	35.0	41.0
27	38.460553078210246	40.0	38.0	41.0	34.0	41.0
28	38.3914732867762	40.0	38.0	41.0	34.0	41.0
29	38.30501531154612	40.0	38.0	41.0	34.0	41.0
30	38.22654252310107	40.0	38.0	41.0	34.0	41.0
31	38.13746608029373	40.0	38.0	41.0	34.0	41.0
32	38.043829362700855	40.0	38.0	41.0	33.0	41.0
33	37.95887826949312	40.0	37.0	41.0	33.0	41.0
34	37.86558731246947	40.0	37.0	41.0	33.0	41.0
35	37.843424951628194	40.0	37.0	41.0	33.0	41.0
36	37.80008698503719	40.0	37.0	41.0	33.0	41.0
37	37.73827311914671	40.0	37.0	41.0	33.0	41.0
38	37.69016187340804	40.0	37.0	41.0	33.0	41.0
39	37.62542142665368	40.0	37.0	41.0	33.0	41.0
40	37.55086692775387	40.0	37.0	41.0	33.0	41.0
41	37.496604314180445	40.0	37.0	41.0	33.0	41.0
42	37.43618736061837	39.0	36.0	41.0	33.0	41.0
43	36.53065132771068	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	1.0
13	1.0
14	0.0
15	0.0
16	6.0
17	9.0
18	27.0
19	64.0
20	104.0
21	209.0
22	357.0
23	664.0
24	1038.0
25	1655.0
26	2456.0
27	3692.0
28	5333.0
29	7419.0
30	10186.0
31	13615.0
32	18224.0
33	24786.0
34	35141.0
35	50959.0
36	80022.0
37	144609.0
38	306825.0
39	301964.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.2318606971286	17.743065221935684	12.20376294496859	26.821311135967125
2	19.88232252030724	19.827932104116535	32.92611522644345	27.363630149132774
3	20.924458746008646	20.887108678788433	28.749644579930628	29.438787995272293
4	15.88130802511272	15.291830836889186	32.406087367454326	36.420773770543775
5	17.008447852073918	33.82449827565539	32.34050183827718	16.826552033993515
6	36.47476789954913	32.72965585430106	15.05574274621075	15.73983349993907
7	31.019280362285745	28.750239010708672	19.91273756178365	20.317743065221936
8	28.57052277214775	31.54961168809424	19.18317285353523	20.69669268622278
9	27.435754416868356	13.737295280516836	18.76340565244227	40.06354465017253
10	18.34353937955297	25.392299545557673	31.662850751310966	24.601310323578392
11	37.8215498989963	20.161110555208253	20.201630919911352	21.815708625884092
12	23.334875550963027	23.900872723453958	27.653415153427535	25.110836572155478
13	32.33663803821992	18.34829482577729	22.725782147064155	26.589284988938633
14	24.1678712145905	19.356944784315747	23.60474712419343	32.87043687690032
15	26.84667351583019	25.70150262193509	20.89374648914322	26.558077373091503
16	26.493581633674108	24.24494907214309	22.799788778930203	26.4616805152526
17	25.433414341038805	24.614387800695287	23.95734364736781	25.994854210898094
18	25.733304668560258	23.55907502608065	24.753979961738473	25.95364034362062
19	26.23817454270936	23.872736333293375	24.61963860590131	25.269450518095958
20	26.566696619373094	23.507953979169162	24.19006329697068	25.73528610448706
21	26.951986835339703	23.528759056400585	23.7685128035436	25.75074130471611
22	27.154588658855182	23.537081087293153	23.640710186264887	25.66762006758678
23	26.658040815598653	23.33764956126055	23.67746582270706	26.326843800433736
24	26.257394471199337	23.759200054687632	23.822308788956267	26.161096685156764
25	26.41046039654477	22.98584561245689	24.036799228032564	26.566894762965777
26	25.94442666656099	23.737404259492813	24.140131111615275	26.17803796233092
27	26.139895320739985	23.664586489182845	23.94228473432412	26.25323345575305
28	26.09006220718092	23.533514502624907	24.314002114192135	26.062421176002037
29	25.69456759619128	23.876996420536	24.372553545829128	26.05588243744359
30	25.76689000751955	23.993504853031943	24.248020297829633	25.991584841618874
31	26.218756470626698	23.61039421658482	23.829838245478115	26.341011067310372
32	25.487903829025853	23.641007401653905	24.415352561848046	26.455736207472192
33	25.54368125036533	23.357364848732228	24.725744499781545	26.3732094011209
34	25.866952521823038	22.943641027216014	24.92705838994461	26.26234806101634
35	25.55626336850052	22.99614907927626	25.32572329841713	26.121864253806095
36	25.2050538504749	22.82871774346151	25.353562473188695	26.612665932874897
37	25.091220356480136	22.03762944968589	25.876364342475348	26.99478585135862
38	24.501247809274904	21.86930646770408	26.836370049010817	26.793075674010197
39	24.45706178810722	21.697119685665005	27.359865420871856	26.485953105355918
40	23.909194754346526	21.55752752462182	28.140848391420782	26.392429329610877
41	23.367272028366237	21.62499541792942	28.508999186620553	26.498733367083794
42	23.047468269780428	21.89437163217812	28.60737748038626	26.45078261765519
43	22.47612122028713	21.48609675946061	28.77659210853513	27.26118991171712
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	65.0
1	72.5
2	80.0
3	160.5
4	241.0
5	241.0
6	336.5
7	432.0
8	416.0
9	400.0
10	593.0
11	786.0
12	786.0
13	1312.0
14	1838.0
15	2797.0
16	3756.0
17	3633.0
18	3510.0
19	3510.0
20	4303.0
21	5096.0
22	5158.5
23	5221.0
24	6354.5
25	7488.0
26	7488.0
27	8956.5
28	10425.0
29	12425.5
30	14426.0
31	17166.0
32	19906.0
33	19906.0
34	23329.5
35	26753.0
36	30659.0
37	34565.0
38	38398.5
39	42232.0
40	42232.0
41	45795.0
42	49358.0
43	52880.0
44	56402.0
45	59464.0
46	62526.0
47	62526.0
48	65162.0
49	67798.0
50	69855.5
51	71913.0
52	73776.0
53	75639.0
54	75639.0
55	75711.0
56	75783.0
57	74867.0
58	73951.0
59	71198.0
60	68445.0
61	68445.0
62	63889.5
63	59334.0
64	54231.5
65	49129.0
66	44278.5
67	39428.0
68	39428.0
69	33864.0
70	28300.0
71	23995.5
72	19691.0
73	16474.0
74	13257.0
75	13257.0
76	10720.0
77	8183.0
78	6817.0
79	5451.0
80	4441.5
81	3432.0
82	3432.0
83	2679.5
84	1927.0
85	1551.5
86	1176.0
87	875.0
88	574.0
89	574.0
90	461.0
91	348.0
92	213.5
93	79.0
94	47.5
95	16.0
96	16.0
97	11.5
98	7.0
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1009369.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.47317809463797
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.33298024260633	66.66770749480365
2	7.411178114941787	11.186903315201697
3	1.8104105736489924	4.099123189482783
4	0.7475827622833793	2.2568978783317957
5	0.4348370091694941	1.6409265517594473
6	0.27108951667020154	1.227599242274367
7	0.1849654754602183	0.9771952589537912
8	0.12877646604058987	0.7775335324703629
9	0.10261347301451633	0.6970108431360525
>10	0.532133536014704	7.070723428620694
>50	0.031499440034435805	1.6054912012557137
>100	0.011408846146347096	1.27862694853838
>500	1.311359922741478E-4	0.05918555559515248
>1k	3.934079768224434E-4	0.4550755595761055
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1738	0.1721867820390759	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1600	0.1585148741441435	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1260	0.12483046338851302	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	9.90717963400897E-5	0.0	0.0	0.0
6	0.0	9.90717963400897E-5	0.0	0.0	0.0
7	0.0	9.90717963400897E-5	0.0	0.0	0.0
8	0.0	9.90717963400897E-5	0.0	0.0	0.0
9	0.0	1.981435926801794E-4	0.0	0.0	0.0
10	0.0	1.981435926801794E-4	0.0	9.90717963400897E-5	0.0
11	0.0	1.981435926801794E-4	0.0	1.981435926801794E-4	0.0
12	0.0	1.981435926801794E-4	0.0	2.972153890202691E-4	0.0
13	0.0	1.981435926801794E-4	0.0	2.972153890202691E-4	0.0
14	0.0	1.981435926801794E-4	0.0	2.972153890202691E-4	0.0
15	0.0	1.981435926801794E-4	0.0	2.972153890202691E-4	0.0
16	0.0	1.981435926801794E-4	0.0	3.962871853603588E-4	0.0
17	0.0	1.981435926801794E-4	0.0	4.953589817004485E-4	0.0
18	0.0	1.981435926801794E-4	0.0	4.953589817004485E-4	0.0
19	0.0	1.981435926801794E-4	0.0	5.944307780405382E-4	0.0
20	0.0	1.981435926801794E-4	0.0	6.935025743806279E-4	0.0
21	0.0	1.981435926801794E-4	0.0	9.90717963400897E-4	0.0
22	0.0	1.981435926801794E-4	0.0	0.001981435926801794	0.0
23	0.0	2.972153890202691E-4	0.0	0.002873082093862601	0.0
24	0.0	2.972153890202691E-4	0.0	0.0060433795767454715	0.0
25	0.0	2.972153890202691E-4	0.0	0.008619246281587804	0.0
26	0.0	2.972153890202691E-4	0.0	0.014761697654673365	0.0
27	0.0	2.972153890202691E-4	0.0	0.0461674570944818	0.0
28	0.0	2.972153890202691E-4	0.0	0.14187081235900845	0.0
29	0.0	2.972153890202691E-4	0.0	0.2697725014340642	0.0
30	0.0	2.972153890202691E-4	0.0	0.4461202989194239	0.0
31	0.0	2.972153890202691E-4	0.0	0.9655537271305142	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	660	0.0	26.628788	1
GCACCGT	80	6.95838E-7	20.8125	6
TGCGAAA	45	0.0038254906	20.555555	10
CTTATAC	1030	0.0	18.859222	37
GTGAGTA	70	0.0025922	15.857143	9
TCTTATA	1710	0.0	15.578947	37
GTATCAA	1140	0.0	15.416666	2
GTGTTAC	90	8.272785E-4	14.388888	1
TATACTG	90	8.272785E-4	14.388888	5
GTAGTAC	90	8.272785E-4	14.388888	3
GTATTAA	195	6.712071E-10	14.23077	1
TAAGGTG	120	3.299574E-5	13.875001	5
GTGTTAG	160	1.7822458E-7	13.875	1
GTGTAAT	80	0.0062979204	13.875	1
GGGCGTT	95	0.0012448896	13.631579	13
CTATACT	150	1.3049284E-6	13.566666	4
AGACTAT	110	2.4558947E-4	13.454545	6
CTCTTAT	2635	0.0	13.19924	37
TACAAGA	155	1.8866631E-6	13.129032	2
CTAGATA	85	0.00940369	13.058824	4
>>END_MODULE