FastQCFastQC Report
Fri 10 Feb 2017
ERR1632241.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632241.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1784543
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34760.19478376256554197No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30070.1685025241756573No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25280.14166091822948507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11900.022.3865551
TCTTATA28800.018.56423637
CTTATAC15100.017.27483637
TAATACT1508.108327E-814.7999994
TCTAATA1252.96078E-614.7999992
TTATACT1304.446989E-614.2307684
GTATCAA18900.013.9973542
ATTACTC1203.3023665E-513.8750013
CGAATTA950.001245545613.63157915
GTGCTAG3150.013.5079371
ACTATAC1805.1630195E-813.3611113
ATAGGAC1551.8889823E-613.1290323
GTATAAA1551.8889823E-613.1290321
TACTATA1857.389099E-813.02
ACATACC1451.372867E-512.758628
ACGCGCG2358.094503E-1012.59574521
CTCTTAT44250.012.37514137
CTAGATT3150.012.3333344
GTATATA1951.4684701E-712.3333341
GATTATA1050.002644555912.3333331
Produced by FastQC (version 0.11.5)